不同温度急性热暴露对肉鸡血清氧化还原指标的影响
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摘要
试验旨在探究不同温度急性热暴露对肉鸡血清氧化还原指标的影响。选取180只34日龄爱拔益加(AA)肉鸡随机分为5组,置于不同的环控舱中进行急性热应激处理,环境温度分别为23、26、29、32、35℃,相对湿度60%,维持设定温度6 h,采集血液用于血清氧化还原指标的测定。结果显示:高温(29℃以上)可引起肉鸡血清中H_2O_2的含量显著升高(P<0.05),小分子抗氧化物质GSH的含量显著减少(P<0.05),而抗氧化酶SOD和CAT的活性随温度升高呈现出先升高后下降趋势(P<0.05),氧自由基O_2~-与-OH的清除率则随环境温度升高显著增加(P<0.05)。结果表明,6 h急性热暴露可使活性氧在体内蓄积,引起肉鸡氧化还原稳态失衡,同时肉鸡体内的抗氧化酶活性升高,表明肉鸡对急性热应激具有一定的耐受性,然而当环境温度过高时(35℃以上),机体抗氧化能力开始下降,仍会导致肉鸡发生氧化应激。
This study was conducted to investigate the effects of acute heat exposure at different temperatures on serum redox index of broilers. A total of 180 34-day-age Arbor Acres(AA) broilers were randomly divided into 5 groups and distributed in different environment chambers for acute heat stress treatment. The temperature of five environment chambers were regulated to 23, 26, 29, 32, 35 ℃ respectively, while maintained relative humidity at 60% was kept for 6 h. Then the blood samples were collected for analytical measurements. The results were showed as follows: When the ambient temperature was above 29℃, the content of H2 O2 in broiler serum was significantly increased(P<0.05)as well as the content of small molecule antioxidant GSH was significantly decreased(P<0.05). Moreover, the activity of antioxidant enzymes SOD and CAT first increased and then decreased with the increase of temperature(P<0.05), and the scavenging rate of oxygen free radical O2-and-OH was significantly increased with the increase of environmental temperature(P<0.05). These results suggested that 6 h acute heat exposure could accumulate the reactive oxygen species in the body,causing the redox homeostasis imbalance in broilers, and the antioxidant enzyme activity in broilers was increased, indicating that broilers had certain tolerance to acute heat stress.When the ambient temperature was too high(above 35℃), the body′s antioxidant capacity began to decline and cause oxidative stress in broilers.
This study was conducted to investigate the effects of acute heat exposure at different temperatures on serum redox index of broilers. A total of 180 34-day-age Arbor Acres(AA) broilers were randomly divided into 5 groups and distributed in different environment chambers for acute heat stress treatment. The temperature of five environment chambers were regulated to 23, 26, 29, 32, 35 ℃ respectively, while maintained relative humidity at 60% was kept for 6 h. Then the blood samples were collected for analytical measurements. The results were showed as follows: When the ambient temperature was above 29℃, the content of H2 O2 in broiler serum was significantly increased(P<0.05)as well as the content of small molecule antioxidant GSH was significantly decreased(P<0.05). Moreover, the activity of antioxidant enzymes SOD and CAT first increased and then decreased with the increase of temperature(P<0.05), and the scavenging rate of oxygen free radical O2-and-OH was significantly increased with the increase of environmental temperature(P<0.05). These results suggested that 6 h acute heat exposure could accumulate the reactive oxygen species in the body,causing the redox homeostasis imbalance in broilers, and the antioxidant enzyme activity in broilers was increased, indicating that broilers had certain tolerance to acute heat stress.When the ambient temperature was too high(above 35℃), the body′s antioxidant capacity began to decline and cause oxidative stress in broilers.
引文
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[9] SIES H. Role of metabolic H2O2generation:Redox signaling and oxidative stress[J]. Journal of Biological Chemistry, 2014, 289(13):8735-8741.
[10]张轶凤,齐智利.热应激条件下机体发生氧化应激的机制[J].动物营养学报,2017,29(9):3051-3058.
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[12] PEREIRA D F, N??S I A. Estimating the thermoneutral zone for broiler breeders using behavioral analysis[J].Computers and Electronics in Agriculture, 2008, 62(1):2-7.
[13]杨语嫣,王雪洁,张敏红,等.升温环境下肉鸡体温和呼吸频率对热中性区上限温度估测[J].中国农业科学,2019,52(3):550-557.
[14] TAO Z, JIN JUN L, DE QIAN W, et al. Effects of heat stress on antioxidant defense system, inflammatory injury,and heat shock proteins of muscovy and pekin ducks:Evidence for differential thermal sensitivities[J]. Cell Stress and Chaperones, 2014, 19(6):895-901.
[15]唐丽.热应激对肉种母鸡繁殖性能、相关生理生化和分子指标的影响[D].北京:中国农业科学院,2013.
[16]苗燕.急性热应激条件下不同脂肪类型对肉仔鸡肌肉品质的影响及其机理[D].北京:中国农业科学院,2007.
[17] HUANG C, JIAO H, SONG Z, et al. Heat stress impairs mitochondria functions and induces oxidative injury in broiler chickens[J]. Journal of Animal Science, 2015, 93(5):2144.
[18] ZHANG J F, HU Z P, LU C H, et al. Dietary curcumin supplementation protects against heat-stress-impaired growth performance of broilers possibly through a mitochondrial pathway[J]. Journal of Animal Science, 2015, 93(4):1656.
[2] YANG L, TAN G Y, FU Y Q, et al. Effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration, ROS production and lipid peroxidation in broiler chickens[J]. Comparative Biochemmistry and Physiology Part C:Toxicology and Pharmacology, 2010, 151(2):204-208.
[3] LIN H, DECUYPERE E, BUYSE J. Acute heat stress induces oxidative stress in broiler chickens[J]. Comparative Biochemistry and Physiology Part A:Molecular and Integrative Physiology, 2006, 144(1):11-17.
[4] ALTAN?, PABU?CUOGLU A, ALTAN A, et al. Effect of heat stress on oxidative stress, lipid peroxidation and some stress parameters in broilers[J]. British Poultry Science, 2003, 44(4):545-550.
[5]冯京海,张敏红,郑姗姗,等.高温及氧化应激对体外培养肉鸡骨骼肌线粒体功能的影响[J].中国农业科学,2008,41(10):3264-3269.
[6] MIRANDA-VIZUETE A, VEAL E A. Caenorhabditis elegans as a model for understanding ROS function in physiology and disease[J]. Redox Biology, 2017, 11(C):708-714.
[7] GERAERT P A, GUILLAUMIN S, LECLERCQ B. Are genetically lean broilers more resistant to hot climate[J].British Poultry Science, 1993, 34(4):643-653.
[8] MUJAHID A, AKIBA Y, WARDEN C H, et al. Sequential changes in superoxide production, anion carriers and substrate oxidation in skeletal muscle mitochondria of heat-stressed chickens[J]. FEBS Letters, 2007, 581(18):3461-3467.
[9] SIES H. Role of metabolic H2O2generation:Redox signaling and oxidative stress[J]. Journal of Biological Chemistry, 2014, 289(13):8735-8741.
[10]张轶凤,齐智利.热应激条件下机体发生氧化应激的机制[J].动物营养学报,2017,29(9):3051-3058.
[11] DIARRA S S, TABUACIRI P. Feeding management of poultry in high environmental temperatures[J]. International Journal of Poultry Science, 2014, 13(11):657-661.
[12] PEREIRA D F, N??S I A. Estimating the thermoneutral zone for broiler breeders using behavioral analysis[J].Computers and Electronics in Agriculture, 2008, 62(1):2-7.
[13]杨语嫣,王雪洁,张敏红,等.升温环境下肉鸡体温和呼吸频率对热中性区上限温度估测[J].中国农业科学,2019,52(3):550-557.
[14] TAO Z, JIN JUN L, DE QIAN W, et al. Effects of heat stress on antioxidant defense system, inflammatory injury,and heat shock proteins of muscovy and pekin ducks:Evidence for differential thermal sensitivities[J]. Cell Stress and Chaperones, 2014, 19(6):895-901.
[15]唐丽.热应激对肉种母鸡繁殖性能、相关生理生化和分子指标的影响[D].北京:中国农业科学院,2013.
[16]苗燕.急性热应激条件下不同脂肪类型对肉仔鸡肌肉品质的影响及其机理[D].北京:中国农业科学院,2007.
[17] HUANG C, JIAO H, SONG Z, et al. Heat stress impairs mitochondria functions and induces oxidative injury in broiler chickens[J]. Journal of Animal Science, 2015, 93(5):2144.
[18] ZHANG J F, HU Z P, LU C H, et al. Dietary curcumin supplementation protects against heat-stress-impaired growth performance of broilers possibly through a mitochondrial pathway[J]. Journal of Animal Science, 2015, 93(4):1656.