多管发酵法测定生活饮用水中总大肠菌群不确定度的评定
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摘要
为了建立生活饮用水中总大肠菌群测定结果的不确定度评定方法,依据JJF 1059.1.2012 《测定不确定度评定与表示》和SN/T4091-2015《食品微生物学测量不确定度评估指南》。对能力验证样品生活饮用水中的总大肠菌群测定结果做了合成不确定度和扩展不确定度评估。结果表明,检验过程中,不确定度来源的主要因素所占分量由大到小排序为:发酵阳性管比例>样品制备>加样>样品稀释>环境温度。检验结果的扩展不确定度为418 MPN/100 mL,取值区间为85729408MPN/100 mL,包含因子k=2。此方法适用于类似条件下总大肠菌群测定不确定度的评定。
This paper established the uncertainty evaluation method for the determination of total coliform flora in drinking water based on JJF 1059.1.2012 "Evaluation and presentation of uncertainty measurement" and SN/T4091-2015 "Guide to the evaluation of uncertainty in food microbiology measurement". The determination results of total coliform flora in domestic drinking water were evaluated for synthetic uncertainty and extended uncertainty. The results showed that during the test process,the weight of main factors of the source of uncertainty from large to small are as follow :the proportion of fermentative positive tubes > sample preparation > sample dilution > sample dilution >ambient temperature. The extended uncertainty of the test result is 418 MPN/100 mL,and the value range is 8572-9408 MPN/100 mL,including the factor K = 2. This method is suitable for evaluating the uncertainty of total coliform under similar conditions.
This paper established the uncertainty evaluation method for the determination of total coliform flora in drinking water based on JJF 1059.1.2012 "Evaluation and presentation of uncertainty measurement" and SN/T4091-2015 "Guide to the evaluation of uncertainty in food microbiology measurement". The determination results of total coliform flora in domestic drinking water were evaluated for synthetic uncertainty and extended uncertainty. The results showed that during the test process,the weight of main factors of the source of uncertainty from large to small are as follow :the proportion of fermentative positive tubes > sample preparation > sample dilution > sample dilution >ambient temperature. The extended uncertainty of the test result is 418 MPN/100 mL,and the value range is 8572-9408 MPN/100 mL,including the factor K = 2. This method is suitable for evaluating the uncertainty of total coliform under similar conditions.
引文
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[2]杨玲玲,李海芳.食品菌落总数测定盲样考核结果不确定度的评定[J].食品安全质量检测学报,2018,9(14):3780-3783.
[3]国家质量技术监督局.JJF1059.1-2012测量不确定度评定与表示[S].北京:中国计量出版社,2013:1-51.
[4]臧慕文.分析测试不确定度的评定与表示(1)[J].分析实验室,2005,24(11):74-79.
[5]国家质量监督检验检疫总局.中华人民共和国国家计量技术规范JJF1001-2011通用计量术语及定义[S].北京:中国质检出版社,2011:16-18.
[6]张雪琰,胡炜,程刚,等.葡萄酒中总酸测量的不确定度评定[J].食品安全质量检测学报,2017,8(11):4216-4219.
[7]蔡玉红,樊慧梅,刘笑笑.可见分光光度法测定紫甘薯中矢车菊色素含量不确定度评估[J].中国食品添加剂,2018(8):188-192.
[8]张贵刚,黄博珠.粪大肠菌群多管发酵法不确定度的评定[J].资源节约与环保,2018(8):37,48.
[9]Internation Orangnization for Standardization(ISO).ISO/IEC17025:2005.General requirements for the competence of testing and calibration laboratories[S].Switzerland:ISOcopyright office:14-15.
[10]中华人民共和国卫生部和中国国家标准化管理委员会.GB/T 5750.12-2006生活饮用水标准检验方法微生物指标[S].
[11]全国流量容量计量技术委员会.JJG 196-2006中华人民共和国国家计量检定规程常用玻璃量器[S].北京:中国计量出版社,2007.
[12]全国流量容量计量技术委员会.JJG 196-2006中华人民共和国国家计量检定规程移液器[S].北京:中国计量出版社,2007.
[13]王叔淳.食品分析质量保证与实验室认可[M].北京:化学工业出版社,2004.
[14]凌云,王李宝,沈辉,等.食品中大肠菌群平板计数结果不确定度的评定[J].安全与检测,2010,26(5):75-77.