摘要
采用二倍稀释法确定了六氢β-酸环糊精包合物对7种测试菌种的最小抑菌浓度(MIC),并以活性较强的金黄色葡萄球菌(MIC为16.25 mg/L)和阪崎肠杆菌(MIC为32.5 mg/L)为指示菌,考察了金属离子、食品添加剂对其抑菌活性的影响。结果表明,除乙型副伤寒沙门氏菌外,包合物对单增李斯特菌、白色念珠菌、乙型溶血性链球菌、阪崎肠杆菌、金黄色葡萄球菌、大肠杆菌均有良好的抑菌活性。其中,Na_2SO_3会提高对阪崎肠杆菌的抑菌活性;金属离子中Ca~(2+)、K~+、Na~+、Mg~(2+)、Zn~(2+)及食品添加剂中Na NO_2、苯甲酸钠对包合物的抑菌活性没有明显影响,Fe~(2+)/Fe~(3+)、Cu~(2+)会使HBA/M-β-CD包合物对两种指示菌的抑菌活性减弱,并呈负相关性。山梨酸钾和H_2O_2在质量浓度分别达到14和0.5 g/L时,包合物对阪崎肠杆菌的抑菌活性有所下降;随着KMnO_4添加浓度的增大,包合物对两种测试菌的抑制活性逐渐减弱,至0.38 g/L时失去抑制活性。
The minimum inhibitory concentration(MIC) of hexahydro-b-acids/methyl-b-cyclodextrin(HBA/M-b-CD) on seven test bacteria was determined by two-fold dilution method. Staphylococcus aureus(MIC=16.25 mg/L) and Enterobacter sakazakii(MIC=32.5 mg/L) were used as indicator bacteria. The effects of metal ions and food additives on its antimicrobial activity were investigated. The results showed that the HBA/M-b-CD inclusion complex had superior antimicrobial activity against Listeria monocytogenes,Candida albicans, b-hemolytic streptococcus, Enterobacter sakazakii, Staphylococcus aureus and Escherichia coli, except S. paratyphi A. Na_2 SO_3 could improve the antibacterial activity of the inclusion complex against Enterobacter sakazakii. Metal ions Ca~(2+), K~+, Na~+, Mg~(2+) and Zn~(2+), Na NO_2 and sodium benzoate had no significant effects on the antibacterial activity of the inclusion complex, conversely,Fe~(2+)/Fe~(3+), Cu~(2+) decreased the antibacterial activities against both indicator bacteria and showed a negative correlation. When the mass concentration of potassium sorbate and H_2 O_2 reached 14 g/L and 0.5 g/L,respectively, the antibacterial activity of the inclusion compound against Enterobacter sakazakii decreased.And the inhibitory activity of the inclusion complex on the two test bacteria gradually decreased with the increase of the concentration of KMnO_4, and it lost its inhibitory activity at 0.38 g/L.
引文
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