海南岛附近海域肉芝软珊瑚的鉴定
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摘要
肉芝软珊瑚属(Sarcophyton)是珊瑚礁生态系统中常见的软珊瑚,具有极强的药用价值.我国肉芝软珊瑚种类资源十分丰富,但目前国内对此种珊瑚的分类研究处于停滞状态,肉芝软珊瑚外部形态差异较小,难以直接进行种类鉴定.本研究采集海南省三亚市西瑁岛、万宁市甘蔗岛和大洲岛珊瑚礁区的26个肉芝软珊瑚样品,根据骨针形态学初步鉴定,并分析线粒体msh1基因和COI基因序列进行种类鉴定.结果显示:所有采集的26个样品,鉴定为5个种,分别为:Sarcophyton cherbonnieri、Sarcophyton crassum、Sarcophyton trocheliophorum、Sarcophyton glaucum和Sarcophyton ehrenbergi.其中S. cherbonnieri和S. crassum为我国新纪录种.本研究为海南岛附近海域肉芝软珊瑚的鉴定提供参考,并为我国软珊瑚种类鉴定和生物多样性的研究提供基础数据.
Sarcophyton is a common soft coral genera in the coral reef ecosystem that is known to have important pharmaceutical value. Despite that Sarcophyton resources are abundant in China,no studies so far have pursued its identification. Moreover,the external morphology similarities of Sarcophyton species make it hard to be differentiated. In this study,a total of 26 samples of Sarcophyton were collected from reef areas in the West Island of SanyaCity,Ganzhe Island and Dazhou Island of Wanning City,Hainan Province. A preliminary sclerites morphological identification,and analysis mitochondrial msh1 and COI gene for species identification,indicated that the collected species belongs to Sarcophyton cherbonnieri,Sarcophyton crassum,Sarcophyton trocheliophorum,Sarcophyton glaucum and Sarcophyton ehrenbergi. Since the soft corals S. cherbonnier and S. crassum are the first report to China,this study provides important baseline data for the identification of soft corals in China.
Sarcophyton is a common soft coral genera in the coral reef ecosystem that is known to have important pharmaceutical value. Despite that Sarcophyton resources are abundant in China,no studies so far have pursued its identification. Moreover,the external morphology similarities of Sarcophyton species make it hard to be differentiated. In this study,a total of 26 samples of Sarcophyton were collected from reef areas in the West Island of SanyaCity,Ganzhe Island and Dazhou Island of Wanning City,Hainan Province. A preliminary sclerites morphological identification,and analysis mitochondrial msh1 and COI gene for species identification,indicated that the collected species belongs to Sarcophyton cherbonnieri,Sarcophyton crassum,Sarcophyton trocheliophorum,Sarcophyton glaucum and Sarcophyton ehrenbergi. Since the soft corals S. cherbonnier and S. crassum are the first report to China,this study provides important baseline data for the identification of soft corals in China.
引文
[1] FEUSSNER K,WAQA T. Five new species of Sarcophyton(Coelenterata:Octocorallia)from the Fiji Islands[J]. South Pacific Journal of Natural&Applied Sciences,2013,31(1):1-26.
[2] FABRICIUS K E,ALDERSLADE P. Soft corals and sea fans:a comprehensive guide to the tropical shallow water genera of the central-west Pacific,the Indian Ocean and the Red Sea[M]. Townsville:Australian Institute of Marine Science(AIMS),2001.
[3] GRANT D M,CHENEY B V. Carbon-13 magnetic resonance. VII. Steric perturbation of the carbon-13 chemical shift[J].Journal of the American Chemical Society,1967,89(21):5 315-5 318.
[4] GRAY M W. Evolution of organellar genomes[J]. Current Opinion in Genetics&Development,1999,9(6):678-687.
[5] LIANG L F,GUO Y W. Terpenes from the soft corals of the genus Sarcophyton:chemistry and biological activities[J]. Chemistry&Biodiversity,2013,10(12):2 161-2 196.
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[7] MCFADDEN C S,FRANCE S C,SNCHEZ J A,et al. A molecular phylogenetic analysis of the Octocorallia(Cnidaria:Anthozoa)based on mitochondrial protein-coding sequences[J]. Molecular Phylogenetics&Evolution,2006,41(3):513-527.
[8] FRANCE S C,HOOVER L L. DNA sequences of the mitochondrial COI,gene have low levels of divergence among deep-sea octocorals(Cnidaria:Anthozoa)[J]. Hydrobiologia,2002,471(1/2/3):149-155.
[9] MCFADDEN C S,SNCHEZ J A,FRANCE S C. Molecular phylogenetic insights into the evolution of Octocorallia:a review[J]. Integrative&Comparative Biology,2010,50(3):389-410.
[10] MCFADDEN C S,BROWN A S,BRAYTON C,et al. Application of DNA barcoding in biodiversity studies of shallow-water octocorals:molecular proxies agree with morphological estimates of species richness in Palau[J]. Coral Reefs,2014,33(2):275-286.
[11] MCFADDEN C S,REYNOLDS A M,JANES M P. DNA barcoding of xeniid soft corals(Octocorallia:Alcyonacea:Xeniidae)from Indonesia:species richness and phylogenetic relationships[J]. Systematics&Biodiversity,2014,12(2):247-257.
[12] MCFADDEN C S,BENAYAHU Y,PANTE E,et al. Limitations of mitochondrial gene barcoding in Octocorallia[J]. Molecular Ecology Resources,2015,11(1):19-31.
[13]李楚璞.南海软珊瑚的研究I.牙龙湾的软珊瑚[J].热带海洋学报,1982,1(2):62-75.
[14]李楚璞.南海软珊瑚的研究II.西沙群岛的豆荚软珊瑚属和肉芝软珊瑚属[C]//中国科学院南海海洋研究所.南海海洋科学集刊第6集.北京:科学出版社,1984:103-119.
[15]马列峰.两种中国南海软珊瑚二萜类化学成分及其生物活性研究[D].杭州:浙江工业大学,2013.
[16]梁林富,郭佳伟,范腾,等.中国南海圆盘肉芝软珊瑚萜类成分研究[J].中草药,2016,47(24):4 331-4 335.
[17]蔡丰源,孙鹏,刘宝姝,等.中国南海圆盘肉芝软珊瑚中的甾醇类成分研究[J].广东药学院学报,2014,30(1):36-39.
[18] JIA R,KURTN T,MNDI A,et al. Biscembranoids formed from anα,β-Unsaturatedγ-Lactone ring as a dienophile:structure revision and establishment of their absolute configurations using theoretical calculations of electronic circular dichroism spectra[J]. Journal of Organic Chemistry,2013,78(7):3 113-3 119.
[19]蓝文健,苏镜娱,曾陇梅.扭曲肉芝软珊瑚的化学成分研究[J].中山大学学报(自然科学版),2005,44(4):59-62.
[20] JIA R,SHI Y H,PEI-MIN H E. Chemical constituents of sarcophyton tortuosum[J]. Chinese Journal of Natural Medicines,2010,8:422-424.
[21]张翠仙,阎素君,张广文,等.肉芝软珊瑚Sarcophyton crassocaule中新的多羟基甾醇的分离[J].高等学校化学学报,2007,28(4):686-688.
[22]孟艳辉,苏镜娱,曾陇梅.南海软肉芝软珊瑚的化学成分研究[J].中国海洋药物,1999,18(3):1-3.
[23]郑其煌,黄起鹏.肉芝软珊瑚素酮的合成[J].科学通报,1992,37(21):2014.
[24]颜忠愈,陈传琼,曾陇梅.南海第氏肉芝软珊瑚化学成分的研究II. Sarcophinone结构的测定[J].热带海洋学报,1985,4(1):82-85
[25] VERSEVELDT J. A revision of the genus Sarcophyton Lesson(Octocorallia,Alcyonacea)[J]. Zoologische Verhandelingen,1982,192:1-91.
[26] SAMIMI-NAMIN K,VAN OFWEGEN L P. Some shallow water octocorals(Coelenterata:Anthozoa)of the Persian Gulf[J].Zootaxa,2009,2 058:1-52.
[27] BENAYAHU Y,PERKOL-FINKEL S. Soft corals(Octocorallia:Alcyonacea)from Southern Taiwan. I. Sarcophyton nanwanensis sp. nov.(Octocorallia:Alcyonacea)[J]. Zoological Studies,2004,43(3):537-547.
[28] BENAYAHU Y,OFWEGEN V L P. New species of Sarcophyton and Lobophytum(Octocorallia:Alcyonacea)from Hong Kong[J]. Zoologische Mededelingen,2009,83(9):3-4.
[29] BENAYAHU Y,OFWEGEN L P V,DAI C F,et al. Diversity,distribution and molecular systematics of octocorals(Coelenterata:Anthozoa)of the Penghu Archipelago,Taiwan[J]. Zoological Studies,2012,51(8):1 529-1 548.
[30] VERSEVELDT J,BENAYAHU Y. On two old and fourteen new species of Alcyonacea(Coelenterata,Octocorallia)from the Red Sea[J]. Zoologische Verhandelingen,1983,208:1-33.
[31] SNCHEZ J A,MCFADDEN C S,FRANCE S C,et al. Molecular phylogenetic analyses of shallow-water Caribbean octocorals[J]. Marine Biology,2003,142(5):975-987.
[32] MCFADDEN C S,TULLIS I D,HUTCHINSON M B,et al. Variation in coding(NADH dehydrogenase subunits 2,3,and 6)and noncoding intergenic spacer regions of the mitochondrial genome in Octocorallia(Cnidaria:Anthozoa)[J]. Marine Biotechnology,2004,7(4):516-526.
[33] PONTKINGDON G,OKADA N A,MACFARLANE J L,et al. Mitochondrial DNA of the coral Sarcophyton glaucum contains a gene for a homologue of bacterial MutS:a possible case of gene transfer from the nucleus to the mitochondrion[J]. Journal of Molecular Evolution,1998,46(4):419-431.
[34] HAVERKORT-YEH R D,MCFADDEN C S,BENAYAHU Y,et al. A taxonomic survey of Saudi Arabian Red Sea Octocorals(Cnidaria:Alcyonacea)[J]. Marine Biodiversity,2013,43(4):279-291.
[35]张秀梅,柳广东,高天翔.绒螯蟹种质资源研究进展[J].中国海洋大学学报(自然科学版),2002,32(4):533-542.
[36] BABCOCK R C,BAIRD A H,PIROMVARAGORN S,et al. Identification of scleractinian coral recruits from Indo-Pacific reefs[J]. Zoological Studies,2003,42(1):211-226.
[37]黄晖,何建国,李秀保,等.柳珊瑚分子系统发育学的研究进展[J].海洋通报,2005,24(2):63-71.
[2] FABRICIUS K E,ALDERSLADE P. Soft corals and sea fans:a comprehensive guide to the tropical shallow water genera of the central-west Pacific,the Indian Ocean and the Red Sea[M]. Townsville:Australian Institute of Marine Science(AIMS),2001.
[3] GRANT D M,CHENEY B V. Carbon-13 magnetic resonance. VII. Steric perturbation of the carbon-13 chemical shift[J].Journal of the American Chemical Society,1967,89(21):5 315-5 318.
[4] GRAY M W. Evolution of organellar genomes[J]. Current Opinion in Genetics&Development,1999,9(6):678-687.
[5] LIANG L F,GUO Y W. Terpenes from the soft corals of the genus Sarcophyton:chemistry and biological activities[J]. Chemistry&Biodiversity,2013,10(12):2 161-2 196.
[6] MCFADDEN C S,ALDERSLADE P,OFWEGEN L P V,et al. Phylogenetic relationships within the tropical soft coral genera Sarcophyton and Lobophytum(Anthozoa,Octocorallia)[J]. Invertebrate Biology,2006,125(4):288-305.
[7] MCFADDEN C S,FRANCE S C,SNCHEZ J A,et al. A molecular phylogenetic analysis of the Octocorallia(Cnidaria:Anthozoa)based on mitochondrial protein-coding sequences[J]. Molecular Phylogenetics&Evolution,2006,41(3):513-527.
[8] FRANCE S C,HOOVER L L. DNA sequences of the mitochondrial COI,gene have low levels of divergence among deep-sea octocorals(Cnidaria:Anthozoa)[J]. Hydrobiologia,2002,471(1/2/3):149-155.
[9] MCFADDEN C S,SNCHEZ J A,FRANCE S C. Molecular phylogenetic insights into the evolution of Octocorallia:a review[J]. Integrative&Comparative Biology,2010,50(3):389-410.
[10] MCFADDEN C S,BROWN A S,BRAYTON C,et al. Application of DNA barcoding in biodiversity studies of shallow-water octocorals:molecular proxies agree with morphological estimates of species richness in Palau[J]. Coral Reefs,2014,33(2):275-286.
[11] MCFADDEN C S,REYNOLDS A M,JANES M P. DNA barcoding of xeniid soft corals(Octocorallia:Alcyonacea:Xeniidae)from Indonesia:species richness and phylogenetic relationships[J]. Systematics&Biodiversity,2014,12(2):247-257.
[12] MCFADDEN C S,BENAYAHU Y,PANTE E,et al. Limitations of mitochondrial gene barcoding in Octocorallia[J]. Molecular Ecology Resources,2015,11(1):19-31.
[13]李楚璞.南海软珊瑚的研究I.牙龙湾的软珊瑚[J].热带海洋学报,1982,1(2):62-75.
[14]李楚璞.南海软珊瑚的研究II.西沙群岛的豆荚软珊瑚属和肉芝软珊瑚属[C]//中国科学院南海海洋研究所.南海海洋科学集刊第6集.北京:科学出版社,1984:103-119.
[15]马列峰.两种中国南海软珊瑚二萜类化学成分及其生物活性研究[D].杭州:浙江工业大学,2013.
[16]梁林富,郭佳伟,范腾,等.中国南海圆盘肉芝软珊瑚萜类成分研究[J].中草药,2016,47(24):4 331-4 335.
[17]蔡丰源,孙鹏,刘宝姝,等.中国南海圆盘肉芝软珊瑚中的甾醇类成分研究[J].广东药学院学报,2014,30(1):36-39.
[18] JIA R,KURTN T,MNDI A,et al. Biscembranoids formed from anα,β-Unsaturatedγ-Lactone ring as a dienophile:structure revision and establishment of their absolute configurations using theoretical calculations of electronic circular dichroism spectra[J]. Journal of Organic Chemistry,2013,78(7):3 113-3 119.
[19]蓝文健,苏镜娱,曾陇梅.扭曲肉芝软珊瑚的化学成分研究[J].中山大学学报(自然科学版),2005,44(4):59-62.
[20] JIA R,SHI Y H,PEI-MIN H E. Chemical constituents of sarcophyton tortuosum[J]. Chinese Journal of Natural Medicines,2010,8:422-424.
[21]张翠仙,阎素君,张广文,等.肉芝软珊瑚Sarcophyton crassocaule中新的多羟基甾醇的分离[J].高等学校化学学报,2007,28(4):686-688.
[22]孟艳辉,苏镜娱,曾陇梅.南海软肉芝软珊瑚的化学成分研究[J].中国海洋药物,1999,18(3):1-3.
[23]郑其煌,黄起鹏.肉芝软珊瑚素酮的合成[J].科学通报,1992,37(21):2014.
[24]颜忠愈,陈传琼,曾陇梅.南海第氏肉芝软珊瑚化学成分的研究II. Sarcophinone结构的测定[J].热带海洋学报,1985,4(1):82-85
[25] VERSEVELDT J. A revision of the genus Sarcophyton Lesson(Octocorallia,Alcyonacea)[J]. Zoologische Verhandelingen,1982,192:1-91.
[26] SAMIMI-NAMIN K,VAN OFWEGEN L P. Some shallow water octocorals(Coelenterata:Anthozoa)of the Persian Gulf[J].Zootaxa,2009,2 058:1-52.
[27] BENAYAHU Y,PERKOL-FINKEL S. Soft corals(Octocorallia:Alcyonacea)from Southern Taiwan. I. Sarcophyton nanwanensis sp. nov.(Octocorallia:Alcyonacea)[J]. Zoological Studies,2004,43(3):537-547.
[28] BENAYAHU Y,OFWEGEN V L P. New species of Sarcophyton and Lobophytum(Octocorallia:Alcyonacea)from Hong Kong[J]. Zoologische Mededelingen,2009,83(9):3-4.
[29] BENAYAHU Y,OFWEGEN L P V,DAI C F,et al. Diversity,distribution and molecular systematics of octocorals(Coelenterata:Anthozoa)of the Penghu Archipelago,Taiwan[J]. Zoological Studies,2012,51(8):1 529-1 548.
[30] VERSEVELDT J,BENAYAHU Y. On two old and fourteen new species of Alcyonacea(Coelenterata,Octocorallia)from the Red Sea[J]. Zoologische Verhandelingen,1983,208:1-33.
[31] SNCHEZ J A,MCFADDEN C S,FRANCE S C,et al. Molecular phylogenetic analyses of shallow-water Caribbean octocorals[J]. Marine Biology,2003,142(5):975-987.
[32] MCFADDEN C S,TULLIS I D,HUTCHINSON M B,et al. Variation in coding(NADH dehydrogenase subunits 2,3,and 6)and noncoding intergenic spacer regions of the mitochondrial genome in Octocorallia(Cnidaria:Anthozoa)[J]. Marine Biotechnology,2004,7(4):516-526.
[33] PONTKINGDON G,OKADA N A,MACFARLANE J L,et al. Mitochondrial DNA of the coral Sarcophyton glaucum contains a gene for a homologue of bacterial MutS:a possible case of gene transfer from the nucleus to the mitochondrion[J]. Journal of Molecular Evolution,1998,46(4):419-431.
[34] HAVERKORT-YEH R D,MCFADDEN C S,BENAYAHU Y,et al. A taxonomic survey of Saudi Arabian Red Sea Octocorals(Cnidaria:Alcyonacea)[J]. Marine Biodiversity,2013,43(4):279-291.
[35]张秀梅,柳广东,高天翔.绒螯蟹种质资源研究进展[J].中国海洋大学学报(自然科学版),2002,32(4):533-542.
[36] BABCOCK R C,BAIRD A H,PIROMVARAGORN S,et al. Identification of scleractinian coral recruits from Indo-Pacific reefs[J]. Zoological Studies,2003,42(1):211-226.
[37]黄晖,何建国,李秀保,等.柳珊瑚分子系统发育学的研究进展[J].海洋通报,2005,24(2):63-71.