氧化石墨烯/适配体-量子点荧光探针用于ATP检测
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摘要
利用量子点标记技术,通过生物偶联方式,以无毒的In P/Zn S表面标记上ATP适配体(ABA-QD)为荧光团,氧化石墨烯(GO)为猝灭剂,二者组装成GO/ABA-QD纳米荧光探针,QD与GO之间产生长程共振能量转移(LrRET),量子点荧光猝灭。当ATP存在时,ATP适配体与其特异结合,构象改变,量子点从GO表面分离,二者之间的荧光共振能量转移被打断,量子点荧光恢复,通过荧光"开"方式检测ATP,检测ATP的检测限为40 nmol/L,线性范围为0.1~30μmol/L。该新型检测ATP的方法对肿瘤的发生、疾病的诊断以及活细胞原位成像等具有重要的意义。
By using In P/Zn S quantum dots( QD) to label ATP binding aptamers( ABA) as fluorophore by biological coupling method,combining with graphene oxide( GO) as quencher,GO/ABA-QD fluorescent nanoprobe was constructed for ATP detection. In GO/ABA-QD,the fluorescence of QD was quenched by GO through long-range resonance energy transfer( LrRET). In the present of ATP,the ABA binding with ATP to form duplex,releasing ABA-QD from GO and interrupting the LrRET,the fluorescence of QD was recovered to achieve ATP detection by fluorescence "turn on "mode. The obtained linear range for ATP was from 0. 1 μmol/L to 30 μmol/L; the detection of limit was estimated to be 40 nmol/L by the GO/ABA-QD fluorescent nanoprobe.
By using In P/Zn S quantum dots( QD) to label ATP binding aptamers( ABA) as fluorophore by biological coupling method,combining with graphene oxide( GO) as quencher,GO/ABA-QD fluorescent nanoprobe was constructed for ATP detection. In GO/ABA-QD,the fluorescence of QD was quenched by GO through long-range resonance energy transfer( LrRET). In the present of ATP,the ABA binding with ATP to form duplex,releasing ABA-QD from GO and interrupting the LrRET,the fluorescence of QD was recovered to achieve ATP detection by fluorescence "turn on "mode. The obtained linear range for ATP was from 0. 1 μmol/L to 30 μmol/L; the detection of limit was estimated to be 40 nmol/L by the GO/ABA-QD fluorescent nanoprobe.
引文
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