北方地区黑木耳部分主栽品种与野生菌株遗传多样性SSR分析
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摘要
收集并采集黑木耳(Auricularia auricula-judae)野生菌株与主栽品种23份,以设计的10对SSR引物对其遗传多样性进行分析。结果表明,10对引物对23个菌株扩增的多态性信息含量在0.050～0.320,均值为0.216,Shannon信息指数0.100～0.690,均值0.455,引物MR005扩增效果最好,多态性最为丰富。对黑木耳23个菌株的聚类分析表明,北方地区黑木耳主栽品种与野生菌株可划分为4类,大部分主栽品种和野生菌株聚到了第三大类群(主栽类群),说明北方地区黑木耳栽培品种与野生菌株间遗传背景差异不大。
A total of 23 wild strains and main cultivars of Auricularia auricula-judae were collected, and the genetic diversity of A. uricularia auricula-judae with 10 pairs of SSR primers was analyzed. The results showed that the content of polymorphism information for 10 pairs of primers was between 0.05 and 0.32, the mean value was 0.216, shannon information index was between0.1 and 0.69, and the mean value was 0.455. Primer MR005 had the best amplification effect and polymorphism was the most abundant. Cluster analysis of 23 strains showed that the main cultivars and wild strains in northern China can be divided into four categories. Most major cultivars and wild strains gathered in third group, named the main cultivated group. It indicated that there was little difference in genetic background between the main cultivars and wild strains in northern China.
A total of 23 wild strains and main cultivars of Auricularia auricula-judae were collected, and the genetic diversity of A. uricularia auricula-judae with 10 pairs of SSR primers was analyzed. The results showed that the content of polymorphism information for 10 pairs of primers was between 0.05 and 0.32, the mean value was 0.216, shannon information index was between0.1 and 0.69, and the mean value was 0.455. Primer MR005 had the best amplification effect and polymorphism was the most abundant. Cluster analysis of 23 strains showed that the main cultivars and wild strains in northern China can be divided into four categories. Most major cultivars and wild strains gathered in third group, named the main cultivated group. It indicated that there was little difference in genetic background between the main cultivars and wild strains in northern China.
引文
[1]李黎.中国木耳栽培种质资源的遗传多样性研究[D].武汉:华中农业大学,2011.
[2]周雁,范秀芝,陈连福,等.SSR在黑木耳和毛木耳转录组中的分布和序列特征[J].菌物学报,2014, 33(2):280-288.
[3]马银鹏,陈鹤,孔祥辉,等.黑龙江省黑木耳主栽品种SSR遗传多样性初探[J].食用菌,2016, 38(5):17-18, 24.
[4]徐安然.黑木耳种质资源的鉴定及SSR分子身份证的构建[D].长春:吉林农业大学,2017.
[5]李瑶瑶,刘云国,刘凌霄,等.基于NCBI核酸数据库资源的产黄青霉微卫星序列的筛选[J].烟台大学学报:自然科学与工程版,2017, 30(3):191-196, 213.
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[7] Moorea SS, Sargeant LL, Kinga TJ, et al. The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species[J]. Genomics, 1991, 10(3):654-660.
[8] Morgantae M, Olivieri AM. PCR-amplified microsatellites as markers in plant genetics[J]. The Plant Journal:for Cell and Molecular Miology, 1993,3(1):175-182.
[9] Field D, Wills C. Long, polymorphic microsatellites in simple organisms[J]. Proceedings. Biological sciences/The Royal Society, 1996, 263(1367):209-215.
[10]鲁绪才.分子标记在我国辣椒种质资源分类上的应用现状及展望[J].现代园艺,2013(7):15-16, 32.
[11]刘子记,曹振木,杨衍.应用分子标记检测作物杂交种纯度研究现状及比较分析[J].长江蔬菜,2013(12):10-15.
[12]王东东,李良秋,马连营,等.大型真菌中SSR分子标记的开发与应用进展[J].微生物学通报,2013, 40(4):646-654.
[13]杨东娟,马瑞君.SSR分子标记在作物遗传多样性研究中的应用现状[J].甘肃科技,2007, 23(8):99-102.
[14]李媛媛,沈金雄,王同华,等.利用SRAP、SSR和AFLP标记构建甘蓝型油菜遗传连锁图谱[J].中国农业科学,2007, 40(6):1118-1126.
[15]杨光.大豆始花期和重要农艺性状QTL定位及相互关系分析[D].沈阳:沈阳农业大学,2017.
[16]周庆友.丝瓜主要农艺性状的遗传分析及种皮颜色基因定位[D].南昌:江西农业大学,2013.
[2]周雁,范秀芝,陈连福,等.SSR在黑木耳和毛木耳转录组中的分布和序列特征[J].菌物学报,2014, 33(2):280-288.
[3]马银鹏,陈鹤,孔祥辉,等.黑龙江省黑木耳主栽品种SSR遗传多样性初探[J].食用菌,2016, 38(5):17-18, 24.
[4]徐安然.黑木耳种质资源的鉴定及SSR分子身份证的构建[D].长春:吉林农业大学,2017.
[5]李瑶瑶,刘云国,刘凌霄,等.基于NCBI核酸数据库资源的产黄青霉微卫星序列的筛选[J].烟台大学学报:自然科学与工程版,2017, 30(3):191-196, 213.
[6]冯立健.黑龙江野生黑木耳种质资源的SSR分析[D].哈尔滨:东北农业大学,2014.
[7] Moorea SS, Sargeant LL, Kinga TJ, et al. The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species[J]. Genomics, 1991, 10(3):654-660.
[8] Morgantae M, Olivieri AM. PCR-amplified microsatellites as markers in plant genetics[J]. The Plant Journal:for Cell and Molecular Miology, 1993,3(1):175-182.
[9] Field D, Wills C. Long, polymorphic microsatellites in simple organisms[J]. Proceedings. Biological sciences/The Royal Society, 1996, 263(1367):209-215.
[10]鲁绪才.分子标记在我国辣椒种质资源分类上的应用现状及展望[J].现代园艺,2013(7):15-16, 32.
[11]刘子记,曹振木,杨衍.应用分子标记检测作物杂交种纯度研究现状及比较分析[J].长江蔬菜,2013(12):10-15.
[12]王东东,李良秋,马连营,等.大型真菌中SSR分子标记的开发与应用进展[J].微生物学通报,2013, 40(4):646-654.
[13]杨东娟,马瑞君.SSR分子标记在作物遗传多样性研究中的应用现状[J].甘肃科技,2007, 23(8):99-102.
[14]李媛媛,沈金雄,王同华,等.利用SRAP、SSR和AFLP标记构建甘蓝型油菜遗传连锁图谱[J].中国农业科学,2007, 40(6):1118-1126.
[15]杨光.大豆始花期和重要农艺性状QTL定位及相互关系分析[D].沈阳:沈阳农业大学,2017.
[16]周庆友.丝瓜主要农艺性状的遗传分析及种皮颜色基因定位[D].南昌:江西农业大学,2013.