IAA对球等鞭金藻和棕鞭藻生长及总脂含量的影响
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摘要
本文研究了0.1~5mg/L的3-吲哚乙酸(IAA)对球等鞭金藻(Isochrysis galbana)和0.1~3mg/L的IAA对棕鞭藻(Ochromonas neopolitana)的叶绿素荧光参数(PSII最大光能转化效率Fv/Fm、相对电子传递速率rETR、光化学淬灭qP)、细胞密度、叶绿素含量、总脂含量及脂肪酸组成的影响。研究表明,球等鞭金藻和棕鞭藻进行光合作用和生长的最适IAA浓度分别为0.1和0.25mg/L。此条件下,2株藻的Fv/Fm、rETR、qP、细胞密度和叶绿素含量均显著高于对照组和其它处理组。其中,球等鞭金藻的细胞密度和叶绿素含量分别比对照组增加了32.6%和37.1%,总脂含量显著高于其它处理组,但与对照组差异不显著(P>0.05);棕鞭藻的细胞密度和叶绿素含量在IAA浓度为0.25mg/L时分别比对照组增加了48.7%和34.4%,总脂含量在IAA浓度为0.5mg/L时显著高于对照组和其它处理组,但与0.25mg/L处理组差异不显著(P>0.05)。0.1mg/L的IAA浓度对球等鞭金藻DHA(二十二碳六烯酸)的合成有促进作用,但对其它各脂肪酸(14:0、16:0、16:1n-7和18:1n-9)含量均无显著影响;0.25mg/L的IAA浓度促进了棕鞭藻DHA和多不饱和脂肪酸的合成。研究结果表明,球等鞭金藻和棕鞭藻进行生长和油脂积累的最适IAA浓度分别为0.1和0.25mg/L,此结果为2株藻的大规模培养提供了理论依据。
Effect of IAA at 0.1~5 and 0.1~3 mg/L on the maximal photochemical efficiency of PSII(Fv/Fm),relative electron transport rate(rETR),photochemical quenching(qP),cell density,chlorophyll content,total lipid content and fatty acid composition of Isochrysis galbana and Ochromonas neopolitana was investigated,respectively.The results showed that the optimal IAA concentration for the photosynthesis and growth of I.galbanaand O.neopolitana was 0.1 and 0.25 mg/L,respectively,at which,Fv/Fm,rETR,qP,cell density and chlorophyll content of two microalgae were all higher than those of control and those at other concentrations.Compared with control,the cell density and chlorophyll content of I.galbanaincreased by 32.6% and 37.1%,respectively.Its total lipid content was higher than that at other concentrations but similar to that of control.For O.neopolitana,the cell density and chlorophyll content at 0.25 mg/L increased by 48.7%and 34.4%,respectively,however the highest total lipid content of this alga was detected at 0.5 mg/L,which was similar to that at 0.25 mg/L.For fatty acid composition,the 0.1 mg/L IAA enhanced the synthesis of DHA,but not other fatty acid components(14:0,16:0,16:1 n-7,18:1 n-9).The 0.25 mg/L IAA enhanced the synthesis of both DHA and other polyunsaturated fatty acids of O.neopolitana.Our findings indicated that the optimal IAA concentration for the growth and oil accumulation of I.galbanaand O.neopolitana was 0.1 mg/L and 0.25 mg/L,respectively,which provided the theoretic basis for large scale cultivation of two microalgae.
Effect of IAA at 0.1~5 and 0.1~3 mg/L on the maximal photochemical efficiency of PSII(Fv/Fm),relative electron transport rate(rETR),photochemical quenching(qP),cell density,chlorophyll content,total lipid content and fatty acid composition of Isochrysis galbana and Ochromonas neopolitana was investigated,respectively.The results showed that the optimal IAA concentration for the photosynthesis and growth of I.galbanaand O.neopolitana was 0.1 and 0.25 mg/L,respectively,at which,Fv/Fm,rETR,qP,cell density and chlorophyll content of two microalgae were all higher than those of control and those at other concentrations.Compared with control,the cell density and chlorophyll content of I.galbanaincreased by 32.6% and 37.1%,respectively.Its total lipid content was higher than that at other concentrations but similar to that of control.For O.neopolitana,the cell density and chlorophyll content at 0.25 mg/L increased by 48.7%and 34.4%,respectively,however the highest total lipid content of this alga was detected at 0.5 mg/L,which was similar to that at 0.25 mg/L.For fatty acid composition,the 0.1 mg/L IAA enhanced the synthesis of DHA,but not other fatty acid components(14:0,16:0,16:1 n-7,18:1 n-9).The 0.25 mg/L IAA enhanced the synthesis of both DHA and other polyunsaturated fatty acids of O.neopolitana.Our findings indicated that the optimal IAA concentration for the growth and oil accumulation of I.galbanaand O.neopolitana was 0.1 mg/L and 0.25 mg/L,respectively,which provided the theoretic basis for large scale cultivation of two microalgae.
引文
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[2]Park W K,Yoo G,Moon M,et al.Phytohormone supplementation significantly increases growth of Chlamydomonas reinhardtii cultivated for biodiesel production[J].Applied Biochemistry and Biotechnology,2013,171(5):1128-1142.
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[5]Allen M F,Moore Jr T S,Christensen M.Phytohormone changes in Bouteloua gracilis infected by vesicular-arbuscular mycorrhizae.II.Altered levels of gibberellin-like substances and abscisic acid in the host plant[J].Canadian Journal of Botany,1982,60(4):468-471.
[6]钱领,刘广发,高亚辉,等.多胺和植物激素对巴氏杜氏藻生长及脂类含量的影响[J].应用海洋学报,2015,34(3):26-28.Qian L,Liu G F,Gao Y H,et al.Influence of phytohormones and polyamines on the growth and lipid content of Dunaliella bardawil[J].Journal of Applied Oceanography,2015,34(3):26-28.
[7]Galun E.Phytohormones and Patterning:the Role of Hormones in Plant Architecture[M].America:World Scientific,2010:189-206.
[8]杨世杰.植物生物学[M].北京:科学出版社,2000.Yang S J.Plant Biology[M].Beijing:Science Press,2000.
[9]Vance B D.Phytohormone effects on cell division in Chlorella pyrenoidosa chick(TX-7-11-05)(Chlorellaceae)[J].Journal of Plant Growth Regulation,1987,5(3):169-173.
[10]Mazur H,Konop A,Synak R.Indole-3-acetic acid in the culture medium of two axenic green microalgae[J].Journal of Applied Phycology,2001,13(1):35-42.
[11]刘海涛,徐志明,陈敏资.三十烷醇对亚心形扁藻生长的影响[J].水产科学,1993,12(3):23-25.Liu H T,Xu Z M,Chen M Z.The effects of triacontanol on the multiplication of Platymonas subcordiformis[J].Fisheries Science,1993,12(3):23-25.
[12]李雅娟,李梅,毛连菊,等.几种植物生长物质对底栖硅藻生长速率的影响[J].大连水产学院学报,1999,14(4):11-16.Li Y J,Li M,Mao L J,et al.Influence of several kinds of plant growth material on the growth rates of benthic diatoms[J].Journal of Dalian Fisheries University,1999,14(4):11-16.
[13]Gonzalez L E,Bashan Y.Increased growth of the microalgae Chlorella vulgaris when coimmobilized and cocultured in alginate beads with the plant-growth-promoting bacterium azospirillum brasilense[J].Applied and Environmental Microbiology,2000,66(4):1527-1531.
[14]孙颖颖,孙利芹,王长海.球等鞭金藻的培养条件研究[J].海洋通报,2005,24(3):92-96.Sun Y Y,Sun L Q,Wang C H.Study on the growth of Isochrysis galbana[J].Marine Science Bulletin,2005,24(3):92-96.
[15]Yoshioka M,Yago T,Yoshie-Stark Y,et al.Effect of high frequency of intermittent light on the growth and fatty acid profile of Isochrysis galbana[J].Aquaculture,2012,338(6):111-117.
[16]Li L,Zhang L,Zhang Z,et al.Comparison of heat resistance and application potential of two lipid-rich Isochrysis galbana strains[J].Algal Research,2016,20:1-6.
[17]范丽敏,梁英,田传远.营养盐浓度对棕鞭藻叶绿素荧光参数、细胞密度和总脂含量的影响[J].水产科学,2012,31(5):249-254.Fan L M,Liang Y,Tian C Y.Effects of nutrient concentration on chlorophyll fluorescence parameters,cell density and total lipid content in algaOchromonas neopolitana[J].Fisheries Science,2012,31(5):249-254.
[18]范丽敏.高脂海洋金藻选育的初步研究[D].青岛:中国海洋大学,2012.Fan L M.Preliminary study on the breeding of high-lipid content marine golden algae[D].Qingdao:Ocean University of China,2012.
[19]Guillard R R L,Ryther J H.Studies of marine planktonic diatoms:I.Cyclotella nana(Hustedt)and Detonula confervacea(Cleve)Gran[J].Canadian Journal of Microbiology,1962,8(2):229-239.
[20]王帅,梁英.Cd2+浓度,温度及交互作用对3株微藻生长及叶绿素荧光特性的影响[J].海洋湖沼通报,2012,5(2):45-58.Wang S,Liang Y.The interaction effects of different Cd2+concentrations and temperature on the growth and chlorophyll fluorescence of 3 microalgae strains[J].Transactions of Oceanology and Limnology,2012,5(2):45-58.
[21]戴荣继,黄春,佟斌,等.藻类叶绿素及其降解产物的测定方法[J].中央民族大学学报(自然科学版),2004,13(1):75-80.Dai R J,Huang C,Tong B,et al.Determination of algal chlorophylls and their degradation products[J].Journal of the Central University for Nationalities(Natural Sciences Edition),2004,13(1):75-80.
[22]Bligh E G,Dyer W J.A rapid method of total lipid extraction and purification[J].Canadian Journal of Biochemistry,1959,37(8):911-917.
[23]Lepage G,Roy C C.Improved recovery of fatty acid through direct transesterification without prior extraction or purification[J].Journal of Lipid Research,1984,25(12):1391-1397.
[24]Li T,Wang C,Miao J.Identification and quantification of indole-3-acetic acid in the kelp Laminaria japonica Areschoug and its effect on growth of marine microalgae[J].Journal of Applied Phycology,2007,19(5):479-484.
[25]王瑶华.富油底栖硅藻的筛选和培养条件的优化[D].上海:上海海洋大学,2014.Wang Y H.Screening and Optimization of Cultivation Condition Oil-rich Benthic Diatom[D].Shanghai:Shanghai Ocean University,2014.
[26]张奕婷,贾文娟,高凤清.几种植物生长调节剂对螺旋藻生长的影响[J].黑龙江农业科学,2010,2010(8):69-70.Zhang Y T,Jia W J,Gao F Q.Effect of several plant growth regulators on the growth of Spirulina[J].Heilongjiang Agricultural Sciences,2010,2010(8):69-70.
[27]李宝光,黄芳.植物叶片面积的测定方法[J].山东理工大学学报:自然科学版,2004,18(4):94-96.Li B G,Huang F.Measurement of plant leaf area[J].Journal of Shandong University of Technology:Natural Sciences Edition,2004,18(4):94-96.
[28]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.Zou Q.Experimental Guide for Plant Physiology[M].Beijing:China Book Review,2000.
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