大孔吸附树脂分离纯化了哥王中西瑞香素的工艺
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摘要
目的探讨大孔吸附树脂纯化分离了哥王中西瑞香素的最佳工艺条件。方法采用高效液相色谱(HPLC)法测定西瑞香素的含量,采用静态吸附和动态吸附结合的方法从8种树脂中优选出一种后对其工艺进行优化。结果 HPD600是纯化了哥王中西瑞香素的优选树脂,最佳吸附条件为上样浓度90μg·m L~(-1),上样液体体积45 m L,上样速度1. 5 m L·min~(-1),吸附率达97. 5%;较佳的洗脱条件:乙醇浓度90%,洗脱剂体积为30 m L,洗脱速度为1.5 m L·min~(-1),解吸率为96.4%。结论大孔吸附树脂HPD600能有效分离纯化了哥王中西瑞香素。
Objective To investigate the best purification conditions of the dephnoretin from Wikstroemia indica Linn by macroporous resin. Methods The content of dephnoretin was determined by HPLC method. The 8 kinds of macroporous resins was selected by static and dynamic adsorption and desorption,and the purification parameters was optimized. Results HPD600 was the best resin to purifiy daphne of Wikstroemia indica Linn; The optimum adsorption conditions for the sample concentration was 90 μg·m L~(-1),the upper sample volume was 45 m L,the sample rate was 1.5 m L·min~(-1),the adsorption rate reached 97.5%; the ethanol concentration was 90% for better conditions of elution,the eluent volume was 30 m L,the elution rate was 1.5 m L · min~(-1),the desorption rate was 96.4%. Conclusion HPD600 macroporous adsorption resin can effectively separate and purify dephnoretin of Wikstroemia indica Linn.
Objective To investigate the best purification conditions of the dephnoretin from Wikstroemia indica Linn by macroporous resin. Methods The content of dephnoretin was determined by HPLC method. The 8 kinds of macroporous resins was selected by static and dynamic adsorption and desorption,and the purification parameters was optimized. Results HPD600 was the best resin to purifiy daphne of Wikstroemia indica Linn; The optimum adsorption conditions for the sample concentration was 90 μg·m L~(-1),the upper sample volume was 45 m L,the sample rate was 1.5 m L·min~(-1),the adsorption rate reached 97.5%; the ethanol concentration was 90% for better conditions of elution,the eluent volume was 30 m L,the elution rate was 1.5 m L · min~(-1),the desorption rate was 96.4%. Conclusion HPD600 macroporous adsorption resin can effectively separate and purify dephnoretin of Wikstroemia indica Linn.
引文
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[2]张婕斐,裘建社,徐新锋,等.了哥王片治疗急性扁桃体炎、急性咽炎、急性气管-支气管炎的临床观察[J].中国医院用药评价与分析,2014,14(3):248-251.
[3]何建芳.了哥王研究进展[J].浙江中西医结合杂志,2001,11(2):129-130.
[4]杨雷军,李爱琦,侯亚琴.了哥王化学成分及临床应用研究进展[J].畜牧兽医科技信息,2011,27(9):8-10.
[5]耿立冬,张村,肖永庆.了哥王化学成分研究[J].中国中药杂志,2006,31(6):817-819.
[6]么焕开,仲英,尹俊亭.了哥王的化学成分研究(Ⅰ)[J].中草药,2007,38(5):669-670.
[7]何宇新,马育彪,李玲,等.反相高效液相色谱法测定不同产地无患子药材中常春藤皂苷元的含量[J].时珍国医国药,2010,21(9):2189-2190.
[8]杨振宇,郭薇,吴东媛,等.了哥王中西瑞香素的提取分离及抗肿瘤作用研究[J].天然产物研究与开发,2008,20(3):522-526.
[9]张立,刘慧琼,肖癑,等.了哥王叶子化学成分研究(Ⅰ)[J].亚太传统医药,2012,8(8):41-43.
[10]陈保红,刘轶华.羊耳菊总黄酮的大孔树脂纯化工艺优化[J].医药导报,2015,34(2):263-266.
[11]杨丽,王宏伟,李洪波,等.大孔树脂法富集黄芩籽种壳总黄酮[J].医药导报,2017,36(4):420-424.
[12]佟立今,孙立新,孙丽霞,等.了哥王化学成分的分离与鉴定[J].中国药物化学杂志,2015,25(1):50-53.
[13]刘慧琼,徐祖疆,王定勇,等.了哥王中西瑞香素对照品的制备及鉴定[J].广东药学院学报,2012,28(1):37-39.
[14]曹增梅,黄和.大孔树脂纯化番石榴多酚的工艺优化[J].食品工业科技,2013,34(7):215-218.