天山假狼毒和瑞香狼毒根中挥发性成分研究
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摘要
目的用GC-MS法分析比较天山假狼毒和瑞香狼毒根中挥发性成分的异同。方法采用索氏提取法提取天山假狼毒和瑞香狼毒根中的挥发性成分,经GC-MS联用仪对挥发性成分进行分析鉴定,采用色谱峰面积归一化法计算各挥发性成分的含量。结果共鉴定出2种植物根中的113个挥发性成分,其中天山假狼毒81个,瑞香狼毒69个,二者有37个共有成分。相对百分含量较高的成分有:十六酸甲酯(hexadecanoic acid-methyl ester)、9,12-顺式十八碳二烯酸[(Z,Z)-9,12-octadecadienoic acid]、角鲨烯(squalene)、1-庚三醇(1-heptatriacotanol)和异柠檬酸乙酯(ethyl iso-allocholate)。结论天山假狼毒和瑞香狼毒根中的挥发性成分有部分相近,但在含量与其他单一组分组成上存在一定的差异,本研究为天山假狼毒和瑞香狼毒药材的质量控制和进一步研究提供了重要理论依据。
Objective To analyze the chemical components of the volatile oil from the roots of Stelleropsis tianschanica and Stellera chamaejasme Linn.,and to compare the difference of components in volatile oil.Methods The volatile oil from the roots of Stelleropsis tianschanica and Stellera chamaejasme Linn.was extracted by using Soxhlet extraction method and analyzed by GC-MS.Its chemical components were calculated by area normalization method.Results 113 compounds were identified in the volatile oil from the roots of Stelleropsis tianschanica and Stellera chamaejasme Linn.,among which 81 compounds were fromStelleropsis tianschanica and 69 compounds were fromStellera chamaejasme Linn.,37 components exist in both of the 2 plants.The relatively high percentage of ingredients were as follows:hexadecanoic acid-methyl ester,(Z,Z)-9,12-octadecadienoic acid,squalene,1-heptatriacotanol and ethyl iso-allocholate.Conclusion The 2 kinds of plants contained plenty of common components,but there are still some differences in composition and content between them.This study provides an important theoretical basis for the quality control and further research of Stelleropsis tianschanica and Stellera chamaejasme Linn..
Objective To analyze the chemical components of the volatile oil from the roots of Stelleropsis tianschanica and Stellera chamaejasme Linn.,and to compare the difference of components in volatile oil.Methods The volatile oil from the roots of Stelleropsis tianschanica and Stellera chamaejasme Linn.was extracted by using Soxhlet extraction method and analyzed by GC-MS.Its chemical components were calculated by area normalization method.Results 113 compounds were identified in the volatile oil from the roots of Stelleropsis tianschanica and Stellera chamaejasme Linn.,among which 81 compounds were fromStelleropsis tianschanica and 69 compounds were fromStellera chamaejasme Linn.,37 components exist in both of the 2 plants.The relatively high percentage of ingredients were as follows:hexadecanoic acid-methyl ester,(Z,Z)-9,12-octadecadienoic acid,squalene,1-heptatriacotanol and ethyl iso-allocholate.Conclusion The 2 kinds of plants contained plenty of common components,but there are still some differences in composition and content between them.This study provides an important theoretical basis for the quality control and further research of Stelleropsis tianschanica and Stellera chamaejasme Linn..
引文
[1]石磊岭,马元甲,古丽娜·沙比尔,等.天山假狼毒和瑞香狼毒叶中挥发性成分的分析研究[J].新疆医科大学学报,2017,40(1):82-85,90.
[2]石磊岭,浩特拉·特克斯,古丽娜·沙比尔,等.天山假狼毒化学成分预实验[J].现代中药研究与实践,2015,29(1):42-43,47.
[3]邓君,刁治民,雷青娟,等.青海省瑞香狼毒的研究现状·综合利用及防治对策[J].安徽农业科学,2006,34(3):555-557.
[4]粟晓黎,林瑞超,王兆基,等.毒性中药狼毒质量标准研究[J].中成药,2006,28(4):498-503.
[5]于保青,胥维昌.瑞香狼毒化学成分及活性的研究进展[J].农药,2008,47(12):863-866,895.
[6]皮立,胡凤祖,韩发,等.藏药瑞香狼毒花挥发油化学成分的气质联用分析[J].时珍国医国药,2012,23(10):2404-2405.
[7]刘文程,王臣.瑞香狼毒的化学成分、生物活性及应用研究进展[J].现代药物与临床,2010,25(1):26-30.
[8]侯婧,张文生,张德春,等.不同提取方法对瑞香科假狼毒属植物挥发性成分分析[J].北京师范大学学报:自然科学版,2007,43(4):414-418.
[9]江苏新医学院.中药大辞典:下册[M].上海:上海科学技术出版社,1977:1898.
[10]魏春雁.瑞香狼毒化学成分与生物活性研究的回顾与展望[J].中国药学杂志,2001,36(9):577-580.
[11]冯宝民,裴月湖.瑞香狼毒中的化学成分研究[J].中国药学杂志,2001,36(1):21-22.
[12]Jiang Z H,Tanaka T,Sakamoto T,et al.Biflavanones,diterpenes,and coumarins from the roots of Stellera chamaejasme L.[J].Chem Pharm Bull(Tokyo),2002,50(1):137-139.
[13]汤亚杰,徐小玲,李冬生,等.瑞香狼毒化学成分与抗肿瘤作用研究进展[J].中成药,2008,30(7):1035-1038.
[14]杨甲月,燕志强,徐蕊,等.瑞香狼毒根中活性物质的分离鉴定及作用机理[J].西北植物学报,2011,31(2):291-297.
[15]王敏,贾正平,马骏,等.瑞香狼毒总黄酮提取物的抗肿瘤作用[J].中国中药杂志,2005,30(8):603-606.
[16]阚晓溪,王志鑫,杨乾栩,等.瑞香狼毒醇提物的抗肿瘤活性研究[J].中国中药杂志,2013,38(8):1219-1225.
[17]宋海平,任韶韶,肖超,等.瑞香狼毒抗肿瘤作用机制研究进展[J].山东医药,2016,56(21):98-101.
[18]周乐,元超,秦宝福,等.瑞香狼毒根的抑菌活性成分研究(Ⅱ)[J].西北植物学报,2004,24(12):2346-2349.
[19]王欣,苏洪丽,李卫敏,等.猫眼草挥发油成分的GC-MS分析[J].西北药学杂志,2016,31(4):353-356.
[20]蔡凡,张芳向,邹振红,等.瑞香狼毒化学成分研究(Ⅴ)挥发油化学成分研究[J].中国现代药物应用,2012,6(19):120-121.
[2]石磊岭,浩特拉·特克斯,古丽娜·沙比尔,等.天山假狼毒化学成分预实验[J].现代中药研究与实践,2015,29(1):42-43,47.
[3]邓君,刁治民,雷青娟,等.青海省瑞香狼毒的研究现状·综合利用及防治对策[J].安徽农业科学,2006,34(3):555-557.
[4]粟晓黎,林瑞超,王兆基,等.毒性中药狼毒质量标准研究[J].中成药,2006,28(4):498-503.
[5]于保青,胥维昌.瑞香狼毒化学成分及活性的研究进展[J].农药,2008,47(12):863-866,895.
[6]皮立,胡凤祖,韩发,等.藏药瑞香狼毒花挥发油化学成分的气质联用分析[J].时珍国医国药,2012,23(10):2404-2405.
[7]刘文程,王臣.瑞香狼毒的化学成分、生物活性及应用研究进展[J].现代药物与临床,2010,25(1):26-30.
[8]侯婧,张文生,张德春,等.不同提取方法对瑞香科假狼毒属植物挥发性成分分析[J].北京师范大学学报:自然科学版,2007,43(4):414-418.
[9]江苏新医学院.中药大辞典:下册[M].上海:上海科学技术出版社,1977:1898.
[10]魏春雁.瑞香狼毒化学成分与生物活性研究的回顾与展望[J].中国药学杂志,2001,36(9):577-580.
[11]冯宝民,裴月湖.瑞香狼毒中的化学成分研究[J].中国药学杂志,2001,36(1):21-22.
[12]Jiang Z H,Tanaka T,Sakamoto T,et al.Biflavanones,diterpenes,and coumarins from the roots of Stellera chamaejasme L.[J].Chem Pharm Bull(Tokyo),2002,50(1):137-139.
[13]汤亚杰,徐小玲,李冬生,等.瑞香狼毒化学成分与抗肿瘤作用研究进展[J].中成药,2008,30(7):1035-1038.
[14]杨甲月,燕志强,徐蕊,等.瑞香狼毒根中活性物质的分离鉴定及作用机理[J].西北植物学报,2011,31(2):291-297.
[15]王敏,贾正平,马骏,等.瑞香狼毒总黄酮提取物的抗肿瘤作用[J].中国中药杂志,2005,30(8):603-606.
[16]阚晓溪,王志鑫,杨乾栩,等.瑞香狼毒醇提物的抗肿瘤活性研究[J].中国中药杂志,2013,38(8):1219-1225.
[17]宋海平,任韶韶,肖超,等.瑞香狼毒抗肿瘤作用机制研究进展[J].山东医药,2016,56(21):98-101.
[18]周乐,元超,秦宝福,等.瑞香狼毒根的抑菌活性成分研究(Ⅱ)[J].西北植物学报,2004,24(12):2346-2349.
[19]王欣,苏洪丽,李卫敏,等.猫眼草挥发油成分的GC-MS分析[J].西北药学杂志,2016,31(4):353-356.
[20]蔡凡,张芳向,邹振红,等.瑞香狼毒化学成分研究(Ⅴ)挥发油化学成分研究[J].中国现代药物应用,2012,6(19):120-121.