中药昆布有效成分的提取分离及抗氧化活性的评价
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摘要
目的研究中药昆布中聚合酚类成分及聚合酚化学成分对斑马鱼高糖模型的细胞保护作用。方法利用硅胶柱层析及sephadex LH-20柱层析等方法进行化学成分纯化,并利用斑马鱼高糖模型研究目标化合物的氧化应激保护作用。结果结合波谱数据和理化性质鉴定化合物的结构为间苯三酚phloroglucinol(1),昆布醇eckol(2),fucodiphloroethol G(3)。结论本研究共分离得到3个聚合酚类化合物。通过斑马鱼高糖模型试验,化合物1和2具有细胞和组织的氧化应激保护作用。
Objective To study on phlorotannins from Ecklonia kurome Okam and their potential oxidative stress protection. Methods Silica column chromatography and sephadex LH-20 column chromatography were used to isolated and purified phenolic components, and using zebrafish sugar model of oxidative stress protection reveals the potential Oxidative stress protection of phlorotannins. Results According to NMR datas and the physical and chemical properties, they show the compound structure as phloroglucinol(1), eckol(2), fucodiphloroethol G(3). Conclusion In Zebrafish sugar model experiment, the results revealed that compounds 1 and 2 for zebrafish sugar cells plays a protective role of the model. Through the above experimental data, we may safely draw the conclusion that compound 1 and 2 showed protection for cells and tissues oxidative stress.
Objective To study on phlorotannins from Ecklonia kurome Okam and their potential oxidative stress protection. Methods Silica column chromatography and sephadex LH-20 column chromatography were used to isolated and purified phenolic components, and using zebrafish sugar model of oxidative stress protection reveals the potential Oxidative stress protection of phlorotannins. Results According to NMR datas and the physical and chemical properties, they show the compound structure as phloroglucinol(1), eckol(2), fucodiphloroethol G(3). Conclusion In Zebrafish sugar model experiment, the results revealed that compounds 1 and 2 for zebrafish sugar cells plays a protective role of the model. Through the above experimental data, we may safely draw the conclusion that compound 1 and 2 showed protection for cells and tissues oxidative stress.
引文
[1]国家药典委员会.中华人民共和国药典:1部[M].北京:中国医药科技出版社,2010:195.
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[5]辛胜昌,赵艳秋,李松,等.斑马鱼模型在药物筛选中的应用[J].遗传,2012,34(9):1144-1152.
[6]张名利,姜云飞,吴操,等.昆布药材中聚合酚类成分研究[J].中国药房,2016,27(15):2111-2113.
[7]LI Yong,QIAN Zhong-ji,RYU B M,et al.Chemical components and its antioxidant properties in vitro:An edible Marine brown alga,Ecklonia cava[J].Bioorganic&Medicinal Chemistry,2009,17(5):1963-1973.
[8]KANG M C,KIN K N,WIJESINGHE W,et al.Protective effect of polyphenol extracted from Ecklonia cava against ethanol induced oxidative damage in vitro and in zebrafish model[J].Journal of Functional Foods,2014(6):339-347.
[2]袁秀丽,吕嘉枥,肖静,等.昆布多糖的研究进展[J].安徽农业科学,2010,38(27):15447-15448,15452.
[3]曾祥丽,丁安伟,昆布的药理研究进展[J].中医药通报,2007(4):63-66.
[4]陈粉丽,张松林,李运彩,斑马鱼胚胎毒理学研究进展[J].湖北农业科学.2010(6),1484-1487.
[5]辛胜昌,赵艳秋,李松,等.斑马鱼模型在药物筛选中的应用[J].遗传,2012,34(9):1144-1152.
[6]张名利,姜云飞,吴操,等.昆布药材中聚合酚类成分研究[J].中国药房,2016,27(15):2111-2113.
[7]LI Yong,QIAN Zhong-ji,RYU B M,et al.Chemical components and its antioxidant properties in vitro:An edible Marine brown alga,Ecklonia cava[J].Bioorganic&Medicinal Chemistry,2009,17(5):1963-1973.
[8]KANG M C,KIN K N,WIJESINGHE W,et al.Protective effect of polyphenol extracted from Ecklonia cava against ethanol induced oxidative damage in vitro and in zebrafish model[J].Journal of Functional Foods,2014(6):339-347.