甘加型藏绵羊发情周期下丘脑GnRH分泌的动态变化规律
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摘要
【目的】研究甘加型藏绵羊发情周期下丘脑促性腺激素释放激素(GnRH)分泌的动态变化及其对藏羊生殖活动的调控.【方法】选取发情周期不同阶段的甘加型藏绵羊48只,采集藏羊发情期、发情后期、间情期、发情前期的血浆.应用酶联免疫吸附试验(ELISA)测定各个时期血浆中GnRH的含量,比较研究其动态变化规律.【结果】甘加藏绵羊发情周期不同阶段血浆中GnRH分泌呈现一定的规律:在发情期和发情后期呈脉冲式分泌,间情期和发情前期既有脉冲式又有波动式分泌.分泌特点为发情前期血浆中GnRH(57.409pg/mL)开始升高,至达到最大值(73.389pg/mL),发情后期(62.058pg/mL)和间情期(55.687pg/mL)分泌量降低,其中发情期分泌量最多,且与其他3个时期差异显著(P<0.05).在整个发情周期中GnRH先后出现4个明显分泌峰,第1个峰出现在发情期4h,第2个峰出现在发情后期60h,第1个峰略高于第2个;第3个峰出现在间情期第9天早上,第4个峰出现在发情前期第14天早上,第3个峰较第4个低.【结论】了解甘加型藏绵羊发情周期下丘脑GnRH分泌的动态变化规律,以及GnRH在甘加型藏绵羊发情周期中的调控作用.
【Objective】In order to study the amount of secretion changes dynamically of the hypothalamus conadotropin-releasing hormone(GnRH)of Ganjia Tibetan sheep at the estrus cycle and the relationship between GnRH and the reproductive activities of Ganjia Tibetan sheep.【Method】There were 48 sheep at different stages of the oestrous cycle,collecting the plasma of oestrus,metestrus,diestrus,proestrus of Ganjia Tibetan sheep.The content of GnRH at different stages of the oestrous cycle was determined by enzyme immune-assay(ELISA)and the dynamic variation rule was studied.【Result】The secretion of GnRH in plasma had certain regularity.The secretion of GnRH was pulsatile at the whole oestrous cycle,the sametime,the diestrus and proestrus was volatility.The characteristic of secretion was the period of estrus increased to the maximum(73.389 pg/mL),the secretion of GnRH reduced in metestrus(62.058 pg/mL)and diestrus(55.687 pg/mL),last the raised in this proestrus(57.409 pg/mL).The secretion volume of GnRH in estrus was the highest and was different from the other three periods(P<0.05).There were four secretory peaks appeared of GnRH in estrous cycle,the 1 st secretory peaks appeared in the estrus 4 h,the2 nd secretory peaks appeared in the metestrus 60 h.The 1 st secretory peak was higher than the 2 nd(P>0.05).The 3 rd secretory peaks appeared in the diestrus 9 th day,the 4 th secretory peaks appeared in the proestrus 14 th day.The 3 rd secretory was lower than the 4 th(P<0.05).【Conclusion】The dynamic changes of secretion of hypothalamus GnRH is revealed,and the regulatory role of GnRH is understood during the estrus cycle of Ganjia Tibetan sheep.
【Objective】In order to study the amount of secretion changes dynamically of the hypothalamus conadotropin-releasing hormone(GnRH)of Ganjia Tibetan sheep at the estrus cycle and the relationship between GnRH and the reproductive activities of Ganjia Tibetan sheep.【Method】There were 48 sheep at different stages of the oestrous cycle,collecting the plasma of oestrus,metestrus,diestrus,proestrus of Ganjia Tibetan sheep.The content of GnRH at different stages of the oestrous cycle was determined by enzyme immune-assay(ELISA)and the dynamic variation rule was studied.【Result】The secretion of GnRH in plasma had certain regularity.The secretion of GnRH was pulsatile at the whole oestrous cycle,the sametime,the diestrus and proestrus was volatility.The characteristic of secretion was the period of estrus increased to the maximum(73.389 pg/mL),the secretion of GnRH reduced in metestrus(62.058 pg/mL)and diestrus(55.687 pg/mL),last the raised in this proestrus(57.409 pg/mL).The secretion volume of GnRH in estrus was the highest and was different from the other three periods(P<0.05).There were four secretory peaks appeared of GnRH in estrous cycle,the 1 st secretory peaks appeared in the estrus 4 h,the2 nd secretory peaks appeared in the metestrus 60 h.The 1 st secretory peak was higher than the 2 nd(P>0.05).The 3 rd secretory peaks appeared in the diestrus 9 th day,the 4 th secretory peaks appeared in the proestrus 14 th day.The 3 rd secretory was lower than the 4 th(P<0.05).【Conclusion】The dynamic changes of secretion of hypothalamus GnRH is revealed,and the regulatory role of GnRH is understood during the estrus cycle of Ganjia Tibetan sheep.
引文
[1]王文芳,何玉琴,韩志磊,等.高原甘加型藏羊发情周期不同阶段卵巢FSHR和LHR基因表达差异的研究[J].中国兽医科学,2016,46(1):97-103.
[2]史军红,何玉琴,葛闻博,等.高原甘加藏羊发情周期垂体FSHβ和LHβmRNA表达差异的研究[J].中国兽医科学,2016,46(11):1444-1449.
[3]王欣荣,吴建平,杨联,等.甘南草地型藏羊体质量与体尺指标的相关性研究[J].甘肃农业大学学报,2011,46(5):7-11.
[4]火静平,何玉琴,韩志磊,等.甘加藏羊发情周期血浆中FSH和LH的分泌规律[J].中国兽医学报,2016,36(6):1053-1058.
[5]郎多勇,李发弟,郎侠,等.甘加型藏羊种质特性研究[J].畜牧兽医杂志,2010,29(3):31-34.
[6]包永清.甘加羊品质资源调查[J].畜牧兽医杂志,2008,27(1):51-52.
[7]KUMAR R S,TRANT J M.Piscine glycoprotein hormone(gonadotropin and thyrotropin)receptors:a review of recent developments[J].Comp Biochem Phys B Biochem Mol Biol,2001,129(2/3):347-355.
[8]KAH O,LETHIMONIER C,SOMOZA G,et al.GnRH and GnRH receptors in metazoan:A historical,Comparative,and evolutive perspective[J].GenComp Endocrinol,2007,153(1/3):346-364.
[9]MILLAR R P,LU Z L,PAWSON A J,et al.Gonadotropin-releasing hormone receptors[J].Endocr Rev,2004,25(2):235-275.
[10]王建辰,王光亚.生殖激素及其在兽医产科中的应用[M].北京:农业出版社,1990:45-62.
[11]杨增明,孙青原,夏国良.生殖生物学[M].北京:科学出版社,2005:13-24.
[12]徐元青,王建林,邵宝平.促性腺激素释放激素(GnRH)结构及调控的研究进展[J].畜牧与饲料科学,2014,3(12):31-33.
[13]吕慎金,刘兆斌.促性腺激素释放激素(GnRH)及其Ⅰ型受体在哺乳动物中的研究进展[J].中国农业大学学报,2013,18(4):147-154.
[14]王一涵,田秀娥,江中良,等.GnRH及其类似物在动物繁殖中的应用概述[J].中国兽医杂志,2013,49(3):89-92.
[15]韩小康.沂蒙黑山羊发情周期卵巢内GTH受体定位及其mRNA表达规律的研究[D].泰安:山东农业大学,2011.
[16]韩志磊,史军红,何玉琴,等.甘加藏羊发情周期阴道细胞变化规律[J].中国兽医学报,2016,36(7):1241-1246.
[17]叶丹,潘建伟,廖鸣娟,等.促性腺激素释放激素的结构及其生物学功能[J].生物化学与生物物理进展,2003(1):49-53.
[18]张克山,胡彦竞科,韩笑哲,等.鹅不同繁殖时期GnRH和GnIH基因表达和激素浓度变化分析[J].畜牧兽医学报,2016,47(8):1720-1726.
[19]章孝荣,王建辰.山羊GnRH和促性腺激素的释放特点[J].中国兽医学报,1997,17(1):177-179.
[20]葛仕豪,高立坤,侯衍猛,等.济宁青山羊发情周期内促性腺激素和性激素分泌规律的研究[J].西南农业学报,2007,20(6):1348-1352.
[21]侯衍猛,曹洪防,徐云华,等.莱芜黑山羊发情周期中FSH LH E2和P的分泌规律[J].中国兽医学报,2006,2(3):340-343.
[22]张贺.不同脉冲频率GnRH刺激对大鼠垂体细胞中FSHβmRNA和LHβmRNA表达水平的影响[D].长春:吉林大学,2007.
[23]桑润滋,李建国,郭刚,等.国产LHR-A3提高细毛羊受胎试验[J].中国畜牧杂志,1995,31(3):39-40.
[24]Foole R H,Allen S E,Henderson.A superovulated oocyte normal[J].Theriogenology,1988,31(1):111-123.
[25]丘奎忠,吴铁荣,柏学进,等.GnRH类似物LRH-A3,配合FSH对超排母牛胚胎质量的影响[J].黑龙江畜牧兽医,1994(3):9-10.
[26]魏锁成,巩转姊,欧阳霞辉,等.GnRH激动剂主动免疫母羊对生殖激素分泌的作用[J].中国免疫学杂志,2012,28(2):132-136.
[2]史军红,何玉琴,葛闻博,等.高原甘加藏羊发情周期垂体FSHβ和LHβmRNA表达差异的研究[J].中国兽医科学,2016,46(11):1444-1449.
[3]王欣荣,吴建平,杨联,等.甘南草地型藏羊体质量与体尺指标的相关性研究[J].甘肃农业大学学报,2011,46(5):7-11.
[4]火静平,何玉琴,韩志磊,等.甘加藏羊发情周期血浆中FSH和LH的分泌规律[J].中国兽医学报,2016,36(6):1053-1058.
[5]郎多勇,李发弟,郎侠,等.甘加型藏羊种质特性研究[J].畜牧兽医杂志,2010,29(3):31-34.
[6]包永清.甘加羊品质资源调查[J].畜牧兽医杂志,2008,27(1):51-52.
[7]KUMAR R S,TRANT J M.Piscine glycoprotein hormone(gonadotropin and thyrotropin)receptors:a review of recent developments[J].Comp Biochem Phys B Biochem Mol Biol,2001,129(2/3):347-355.
[8]KAH O,LETHIMONIER C,SOMOZA G,et al.GnRH and GnRH receptors in metazoan:A historical,Comparative,and evolutive perspective[J].GenComp Endocrinol,2007,153(1/3):346-364.
[9]MILLAR R P,LU Z L,PAWSON A J,et al.Gonadotropin-releasing hormone receptors[J].Endocr Rev,2004,25(2):235-275.
[10]王建辰,王光亚.生殖激素及其在兽医产科中的应用[M].北京:农业出版社,1990:45-62.
[11]杨增明,孙青原,夏国良.生殖生物学[M].北京:科学出版社,2005:13-24.
[12]徐元青,王建林,邵宝平.促性腺激素释放激素(GnRH)结构及调控的研究进展[J].畜牧与饲料科学,2014,3(12):31-33.
[13]吕慎金,刘兆斌.促性腺激素释放激素(GnRH)及其Ⅰ型受体在哺乳动物中的研究进展[J].中国农业大学学报,2013,18(4):147-154.
[14]王一涵,田秀娥,江中良,等.GnRH及其类似物在动物繁殖中的应用概述[J].中国兽医杂志,2013,49(3):89-92.
[15]韩小康.沂蒙黑山羊发情周期卵巢内GTH受体定位及其mRNA表达规律的研究[D].泰安:山东农业大学,2011.
[16]韩志磊,史军红,何玉琴,等.甘加藏羊发情周期阴道细胞变化规律[J].中国兽医学报,2016,36(7):1241-1246.
[17]叶丹,潘建伟,廖鸣娟,等.促性腺激素释放激素的结构及其生物学功能[J].生物化学与生物物理进展,2003(1):49-53.
[18]张克山,胡彦竞科,韩笑哲,等.鹅不同繁殖时期GnRH和GnIH基因表达和激素浓度变化分析[J].畜牧兽医学报,2016,47(8):1720-1726.
[19]章孝荣,王建辰.山羊GnRH和促性腺激素的释放特点[J].中国兽医学报,1997,17(1):177-179.
[20]葛仕豪,高立坤,侯衍猛,等.济宁青山羊发情周期内促性腺激素和性激素分泌规律的研究[J].西南农业学报,2007,20(6):1348-1352.
[21]侯衍猛,曹洪防,徐云华,等.莱芜黑山羊发情周期中FSH LH E2和P的分泌规律[J].中国兽医学报,2006,2(3):340-343.
[22]张贺.不同脉冲频率GnRH刺激对大鼠垂体细胞中FSHβmRNA和LHβmRNA表达水平的影响[D].长春:吉林大学,2007.
[23]桑润滋,李建国,郭刚,等.国产LHR-A3提高细毛羊受胎试验[J].中国畜牧杂志,1995,31(3):39-40.
[24]Foole R H,Allen S E,Henderson.A superovulated oocyte normal[J].Theriogenology,1988,31(1):111-123.
[25]丘奎忠,吴铁荣,柏学进,等.GnRH类似物LRH-A3,配合FSH对超排母牛胚胎质量的影响[J].黑龙江畜牧兽医,1994(3):9-10.
[26]魏锁成,巩转姊,欧阳霞辉,等.GnRH激动剂主动免疫母羊对生殖激素分泌的作用[J].中国免疫学杂志,2012,28(2):132-136.