不同发育时期小鼠腓肠肌Myostatin表达的研究
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摘要
肌生成抑制素(myostatin,MSTN)是肌肉生长发育的反向调节因子,在骨骼肌中大量表达,其在成肌细胞的增殖、分化和融合过程当中扮演着重要的调控作用。本试验通过HE染色、免疫组织化学和PCR等技术研究了各个发育阶段小鼠腓肠肌中Myostatin的定位及其表达差异。结果,Myostatin在幼年、性成熟、体成熟和老年4个时期的小鼠腓肠肌中均有表达,且表达量存在明显的差异,Myostatin在腓肠肌中表达量最高的是在体成熟时期,最低的是在幼年时期,而在性成熟与老年时期的表达量则低于体成熟时期的小鼠。上述结果表明,Myostatin基因可能参与了小鼠骨骼肌的生长发育,这为提高肉用家畜产肉率和防治肌肉疾病提供了理论基础。
Myostatin(MSTN),a negative regulatory factor for muscle growth and development,is expressed in skeletal muscle and plays an important role in the proliferation,differentiation and fusion of myoblasts.In this experiment,HE staining,immunohistochemical and PCR techniques were used to study the localization and differential expression of myostatin in the gastrocnemius of mice at different developmental stages.In result,myostatin was expressed in mice gastrocnemius of young,sexual maturation,body maturation and aged,and there was significant difference in the expression of myostatin.It was the highest in body maturation mice,the lowest in young mice,but slightly lower in sexual maturation and aged mice than in body maturation mice.The above-mentioned results showed that the myostatin gene may be involved in the growth and development of mice skeletal muscle,which provided a theoretical basis for improving the meat production of domestic animals and the prevention and treatment of muscle diseases in animal.
Myostatin(MSTN),a negative regulatory factor for muscle growth and development,is expressed in skeletal muscle and plays an important role in the proliferation,differentiation and fusion of myoblasts.In this experiment,HE staining,immunohistochemical and PCR techniques were used to study the localization and differential expression of myostatin in the gastrocnemius of mice at different developmental stages.In result,myostatin was expressed in mice gastrocnemius of young,sexual maturation,body maturation and aged,and there was significant difference in the expression of myostatin.It was the highest in body maturation mice,the lowest in young mice,but slightly lower in sexual maturation and aged mice than in body maturation mice.The above-mentioned results showed that the myostatin gene may be involved in the growth and development of mice skeletal muscle,which provided a theoretical basis for improving the meat production of domestic animals and the prevention and treatment of muscle diseases in animal.
引文
[1]甘尚权,刘守仁,王新华,等.MSTN基因在动物科学和医学中的研究进展[J].草食家畜,2006(2):24-26.GAN Shang-quan,LIU Shou-ren,WANG Xin-hua,et al.Progresses on studies of myostatin gene applied in animal science and medicine areas[J].Herbivorous Livestock,2006(2):24-26.(in Chinese)
[2]赵瑞媛,时培文,刘春霞,等.蒙古绵羊肌肉生长抑制素基因敲除载体的构建[J].中国兽医科学,2015,45(6):654-660.ZHAO Rui-yuan,SHI Pei-wen,LIU Chun-xia,et al.Construction of knock-out vectors for Mongolian sheep MSTN gene[J].Chinese Veterinary Science,2015,45(6):654-660.(in Chinese)
[3]姬云涛,陆娟,刘生杰,等.MSTN与骨骼肌生长发育[J].中国组织化学与细胞化学杂志,2010,19(6):610-614.JI Yun-tao,LU Juan,LIU Sheng-jie,et al.MSTN and skeletal muscle growth and development[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(6):610-614.(in Chinese)
[4]叶建伟.Myostatin对成肌细胞糖代谢的影响[D].北京:中国协和医科大学,2006.YE Jian-wei.Effects of myostatin on glucose metabolism in myoblasts[D].Beijing:China Union Medical University,2006.(in Chinese)
[5]孙景权,上官若男,李顺昌,等.Myostatin对骨骼肌、褐色脂肪和白色脂肪组织作用及其机制研究进展[J].生理科学进展,2015,46(5):391-396.SUN Jing-quan,SHANGGUAN Ruo-nan,LI Shun-chang,et al.Effects and mechanisms of myostatin on skeletal muscle,brown fat and white fat tissue[J].Advances in Physiology,2015,46(5):391-396.(in Chinese)
[6]BHATT K,WELLE S,PINKERT C A,et al.Muscle growth after postdevelopmental myostatin gene knockout[J].American Journal of Physiology-Endocrinology and Metabolism,2007,292(4):985-991.
[7]王建起,曹文广.Myostatin基因及其与动物双肌性状间关系的研究进展[J].中国农业科学,2014,47(8):1577-1587.WANG Jian-qi,CAO Wen-guang,Myostatin and its double-muscling phenotype in animals[J].Chinese Agricultural Science,2014,47(8):1577-1587.(in Chinese)
[8]JI S,LOSINSKI R L,CORNELIUS S G.Myostatin expression in porcine tissues:tissue specificity and developmental and postnatal regulation[J].American Journal of Physiology,1998,275(4 Pt 2):R1265-R1273.
[9]刘蓉芳.绵羊Myostatin基因cDNA全序列的克隆及在CHO中的表达[D].石河子:石河子大学,2007.LIU Rong-fang.cDNA Sequence of myostatin gene of sheep cloning and expression in CHO[D].Shihezi:Shihezi University,2007.(in Chinese)
[10]BONDESTAM J,HORELLI-KUITUNEN N,RITVOS O,et al.Assignment1 of ACVR2 and ACVR2B the human activin receptor typeⅡandⅡB genes to chromosome bands2q22.2->q23.3 and 3p22 and the human follistatin gene(FST)to chromosome 5q11.2 by FISH[J].Cytogenetic&Genome Research,1999,87(3-4):219-220.
[11]WINBANKS C E,WEEKS K L,THOMSON R E,et al.Follistatin-mediated skeletal muscle hypertrophy is regulated by Smad3 and mTOR independently of myostatin[J].Journal of Cell Biology,2012,197(7):997-1008.
[12]濮剑威.草鱼肌肉生长抑制素基因克隆及功能研究[D].上海:上海海洋大学,2011.PU Jian-wei.Cloning and functional study of myostatin gene in grass carp[D].Shanghai:Shanghai Ocean University,2011.(in Chinese)
[13]刘瑞凿,刘真,吴明明,等.绵羊胚胎发育中后期骨骼肌BTG2/3的表达动态及其与Myostatin的关系[J].畜牧兽医学报,2015,46(11):1916-1923.LIU Rui-zao,LIU Zhen,WU Ming-ming,et al.Expression trend of BTG2/3 in the mid to late embryonic skeletal muscle of sheep and the relationship between BTG2/3 and MSTN[J].Journal of Animal Husbandry and Veterinary Medicine,2015,46(11):1916-1923.(in Chinese)
[14]LIN J,ARNOLD H B,DELLAFERA M A,et al.Myostatin knockout in mice increases myogenesis and decreases adipogenesis[J].Biochemical&BiophysicalResearchCommunications,2002,291(3):701-706.
[15]LEE S J.Regulation of muscle mass by myostatin[J].Annual Review of Cell and Deveolpment Biology,2004,20(1):61-86.
[16]TSUCHIDA K.Targeting myostatin for therapies against muscle-wasting disorders[J].Current Opinion in Drug Discovery&Development,2008,11(4):487-494.
[17]SIRIETT V,SALERNO M S,BERRY C,et al.Antagonism of myostatin enhances muscle regeneration during sarcopenia[J].Molecular Therapy the Journal of the American Society of Gene Therapy,2007,15(8):1463-1470.
[2]赵瑞媛,时培文,刘春霞,等.蒙古绵羊肌肉生长抑制素基因敲除载体的构建[J].中国兽医科学,2015,45(6):654-660.ZHAO Rui-yuan,SHI Pei-wen,LIU Chun-xia,et al.Construction of knock-out vectors for Mongolian sheep MSTN gene[J].Chinese Veterinary Science,2015,45(6):654-660.(in Chinese)
[3]姬云涛,陆娟,刘生杰,等.MSTN与骨骼肌生长发育[J].中国组织化学与细胞化学杂志,2010,19(6):610-614.JI Yun-tao,LU Juan,LIU Sheng-jie,et al.MSTN and skeletal muscle growth and development[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(6):610-614.(in Chinese)
[4]叶建伟.Myostatin对成肌细胞糖代谢的影响[D].北京:中国协和医科大学,2006.YE Jian-wei.Effects of myostatin on glucose metabolism in myoblasts[D].Beijing:China Union Medical University,2006.(in Chinese)
[5]孙景权,上官若男,李顺昌,等.Myostatin对骨骼肌、褐色脂肪和白色脂肪组织作用及其机制研究进展[J].生理科学进展,2015,46(5):391-396.SUN Jing-quan,SHANGGUAN Ruo-nan,LI Shun-chang,et al.Effects and mechanisms of myostatin on skeletal muscle,brown fat and white fat tissue[J].Advances in Physiology,2015,46(5):391-396.(in Chinese)
[6]BHATT K,WELLE S,PINKERT C A,et al.Muscle growth after postdevelopmental myostatin gene knockout[J].American Journal of Physiology-Endocrinology and Metabolism,2007,292(4):985-991.
[7]王建起,曹文广.Myostatin基因及其与动物双肌性状间关系的研究进展[J].中国农业科学,2014,47(8):1577-1587.WANG Jian-qi,CAO Wen-guang,Myostatin and its double-muscling phenotype in animals[J].Chinese Agricultural Science,2014,47(8):1577-1587.(in Chinese)
[8]JI S,LOSINSKI R L,CORNELIUS S G.Myostatin expression in porcine tissues:tissue specificity and developmental and postnatal regulation[J].American Journal of Physiology,1998,275(4 Pt 2):R1265-R1273.
[9]刘蓉芳.绵羊Myostatin基因cDNA全序列的克隆及在CHO中的表达[D].石河子:石河子大学,2007.LIU Rong-fang.cDNA Sequence of myostatin gene of sheep cloning and expression in CHO[D].Shihezi:Shihezi University,2007.(in Chinese)
[10]BONDESTAM J,HORELLI-KUITUNEN N,RITVOS O,et al.Assignment1 of ACVR2 and ACVR2B the human activin receptor typeⅡandⅡB genes to chromosome bands2q22.2->q23.3 and 3p22 and the human follistatin gene(FST)to chromosome 5q11.2 by FISH[J].Cytogenetic&Genome Research,1999,87(3-4):219-220.
[11]WINBANKS C E,WEEKS K L,THOMSON R E,et al.Follistatin-mediated skeletal muscle hypertrophy is regulated by Smad3 and mTOR independently of myostatin[J].Journal of Cell Biology,2012,197(7):997-1008.
[12]濮剑威.草鱼肌肉生长抑制素基因克隆及功能研究[D].上海:上海海洋大学,2011.PU Jian-wei.Cloning and functional study of myostatin gene in grass carp[D].Shanghai:Shanghai Ocean University,2011.(in Chinese)
[13]刘瑞凿,刘真,吴明明,等.绵羊胚胎发育中后期骨骼肌BTG2/3的表达动态及其与Myostatin的关系[J].畜牧兽医学报,2015,46(11):1916-1923.LIU Rui-zao,LIU Zhen,WU Ming-ming,et al.Expression trend of BTG2/3 in the mid to late embryonic skeletal muscle of sheep and the relationship between BTG2/3 and MSTN[J].Journal of Animal Husbandry and Veterinary Medicine,2015,46(11):1916-1923.(in Chinese)
[14]LIN J,ARNOLD H B,DELLAFERA M A,et al.Myostatin knockout in mice increases myogenesis and decreases adipogenesis[J].Biochemical&BiophysicalResearchCommunications,2002,291(3):701-706.
[15]LEE S J.Regulation of muscle mass by myostatin[J].Annual Review of Cell and Deveolpment Biology,2004,20(1):61-86.
[16]TSUCHIDA K.Targeting myostatin for therapies against muscle-wasting disorders[J].Current Opinion in Drug Discovery&Development,2008,11(4):487-494.
[17]SIRIETT V,SALERNO M S,BERRY C,et al.Antagonism of myostatin enhances muscle regeneration during sarcopenia[J].Molecular Therapy the Journal of the American Society of Gene Therapy,2007,15(8):1463-1470.