气相色谱法测定蕲艾移栽品中桉油精的含量
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摘要
建立了一种利用气相色谱法快速测定艾叶中桉油精含量的方法。气相色谱柱为HP-5毛细管柱(30 m×0.32 mm,0.25μm);氢火焰离子化检测器(FID)温度为250℃;进样口温度:240℃;程序升温:柱温起始温度为50℃,以5℃/min升至75℃,保持6 min,再以20℃/min升至200℃,保持3 min。该方法操作简单、快捷,精密度、重复性、稳定性、准确度的RSD≤1.83%;平均加样回收率为96.03%,RSD为1.78%(n=6)。蕲艾在湖南的移栽品桉油精含量变化不大。
A method of rapid determination of eucalyptol in Artemisia argyi was established. Gas chromatography was used with a capillary column HP-5(30 m × 0. 32 mm,0. 25 μm). The FID detector temperature was 250 ℃. The temperature of the inlet was 240 ℃. Temperature programming started at 50 ℃,then raised to 75 ℃ with a rate of 5 ℃/min,mainted for 6 min,and at last raised to 200 ℃ with a rate of 20 ℃/min,mainted for 3 min. The method was simple and rapid. RSDs of precision,repeatability,stability and accuracy were no more than 1. 83%,and the mean recovery was 96. 03%with RSD of 1. 78%(n = 6). The content of eucalyptol in Artemisia argyi's Hunan transplant didn't change much.
A method of rapid determination of eucalyptol in Artemisia argyi was established. Gas chromatography was used with a capillary column HP-5(30 m × 0. 32 mm,0. 25 μm). The FID detector temperature was 250 ℃. The temperature of the inlet was 240 ℃. Temperature programming started at 50 ℃,then raised to 75 ℃ with a rate of 5 ℃/min,mainted for 6 min,and at last raised to 200 ℃ with a rate of 20 ℃/min,mainted for 3 min. The method was simple and rapid. RSDs of precision,repeatability,stability and accuracy were no more than 1. 83%,and the mean recovery was 96. 03%with RSD of 1. 78%(n = 6). The content of eucalyptol in Artemisia argyi's Hunan transplant didn't change much.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010:82-83.
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[9]胡吉清,夏恒建,郭双喜,等.蕲艾挥发油、总黄酮和鞣质含量测定及最佳采收期确定[J].中华中医药杂志,2016,31(8):3013-3016.
[10]甘昌胜,尹彬彬,张靖华,等.艾叶精油蒸馏制取对相应水提液活性成分的影响及其抑菌性能比较[J].食品与生物技术学报,2015,34(12):1327-1331.
[11]Zheng X H,Deng C H,Song G X,et al.Comparison of essential oil composition of Artemisia argyi leaves at different collection times by headspace solid-phase microextraction and gas chroma-tographymass spectrometry[J].Chromatographia,2004,59(11/12):729-732.
[12]Zhang L B,Lv J L,Chen H L,et al.Chemical constituents from Artemisia argyi and their chemotaxonomic significance[J].Bio-chem Syst Ecol,2013,50:455-458.
[13]Zhang W J,You C X,Yang K,et al.Bioactivity of essential oil of Artemisia argyi Levl.et Vant.and its main compounds against Lasioderma serricorne[J].J Oleo Sci,2014,63(8):829-837.
[14]靳然,于密密,赵百孝.气相色谱法测定艾叶4个挥发性成分的含量[J].药物分析杂志,2013,33(6):1033-1036.
[15]Zheng X H,Deng C H,Song G X,et al.Comparison of essential oil composition of Artemisia argyi leaves at different collection times by headspace solid-phase microextraction and gas chroma-tographymass spectrometry[J].Chromatographia,2004,59(11/12):729-732.
[16]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:89.
[2]李真真,吕洁丽,张来宾,等.艾叶的化学成分及药理作用研究进展[J].国际药学研究杂志2016,43(6):1059-1066.
[3]王珊珊,成绍武,宋祯彦.等.艾叶提取物激活凝血因子Ⅻ发挥止血活血功能[J].中华中医药学刊,2017,35(10):1063-1068.
[4]瞿燕,秦旭华,潘晓丽.艾叶和醋艾叶炭止血、镇痛作用比较研究[J].中药药理与临床,2005,21(4):46-47.
[5]蒋潇,田静.三个产地艾叶挥发油的化学成分分析[J].中国民族民间医药,2015,24(17):19-22.
[6]戴卫波,李拥军,梅全喜.12个不同产地艾叶挥发油的GC-MS分析[J].中药材,2015,38(12):2502-2506.
[7]梅全喜.蕲艾的历史地位与现代研究[J].中药材,1995,18(8):426-429.
[8]靳然,于密密,赵百孝,等.不同年份蕲艾叶及不同比例艾绒化学成分研究[J].中国针灸,2010,30(5):389-392.
[9]胡吉清,夏恒建,郭双喜,等.蕲艾挥发油、总黄酮和鞣质含量测定及最佳采收期确定[J].中华中医药杂志,2016,31(8):3013-3016.
[10]甘昌胜,尹彬彬,张靖华,等.艾叶精油蒸馏制取对相应水提液活性成分的影响及其抑菌性能比较[J].食品与生物技术学报,2015,34(12):1327-1331.
[11]Zheng X H,Deng C H,Song G X,et al.Comparison of essential oil composition of Artemisia argyi leaves at different collection times by headspace solid-phase microextraction and gas chroma-tographymass spectrometry[J].Chromatographia,2004,59(11/12):729-732.
[12]Zhang L B,Lv J L,Chen H L,et al.Chemical constituents from Artemisia argyi and their chemotaxonomic significance[J].Bio-chem Syst Ecol,2013,50:455-458.
[13]Zhang W J,You C X,Yang K,et al.Bioactivity of essential oil of Artemisia argyi Levl.et Vant.and its main compounds against Lasioderma serricorne[J].J Oleo Sci,2014,63(8):829-837.
[14]靳然,于密密,赵百孝.气相色谱法测定艾叶4个挥发性成分的含量[J].药物分析杂志,2013,33(6):1033-1036.
[15]Zheng X H,Deng C H,Song G X,et al.Comparison of essential oil composition of Artemisia argyi leaves at different collection times by headspace solid-phase microextraction and gas chroma-tographymass spectrometry[J].Chromatographia,2004,59(11/12):729-732.
[16]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:89.