壮瑶药小槐花挥发油成分气相色谱-质谱联用分析
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摘要
目的:研究壮瑶药小槐花中挥发油成分。方法:采用水蒸气蒸馏法提取小槐花的挥发油成分,用气相色谱-质谱联用技术进行测定,结合计算机检索技术对分离的化合物进行结构鉴定并用色谱峰面积归一化法获得各化合物的相对含量。结果:从小槐花中鉴定出46个成分,占挥发油总量的89. 50%,相对含量较高的挥发油成分有薄荷脑(12. 06%)、邻苯二甲酸二异丁酯(8. 15%)、己酸丁酯(5. 66%)、棕榈酸(24. 40%)、亚油酸(5. 17%)。结论:该方法结果准确可靠,为合理利用小槐花植物资源及其深度研究开发提供实验依据。
Objective To study and detect volatile oil compounds of Desmodium caudatum( Thunb.) DC. Methods The volatile oil components of Desmodium caudatum( Thunb.) DC. was extracted by steam distillation,GC/MS was used for the determination.The structures of the compounds were identified by computer retrieval technique and the relative content of the compounds was obtained by normalization of the chromatographic peak area. Results 46 components were identified from Desmodium caudatum( Thunb.)DC.,accounting for 89. 50% of the total volatile oil,and the volatile oil with relative high content were DL-Mentho( 12. 06%),Diisobutyl phthalate( 8. 15%),Butyl isobutyl phthalate( 5. 66%) 、Palmitic acid( 24. 40%),Linoleic acid( 5. 17%). Conclusion The method is accurate and reliable,and provides the experimental basis for the rational utilization of plant resources and their deep research and development.
Objective To study and detect volatile oil compounds of Desmodium caudatum( Thunb.) DC. Methods The volatile oil components of Desmodium caudatum( Thunb.) DC. was extracted by steam distillation,GC/MS was used for the determination.The structures of the compounds were identified by computer retrieval technique and the relative content of the compounds was obtained by normalization of the chromatographic peak area. Results 46 components were identified from Desmodium caudatum( Thunb.)DC.,accounting for 89. 50% of the total volatile oil,and the volatile oil with relative high content were DL-Mentho( 12. 06%),Diisobutyl phthalate( 8. 15%),Butyl isobutyl phthalate( 5. 66%) 、Palmitic acid( 24. 40%),Linoleic acid( 5. 17%). Conclusion The method is accurate and reliable,and provides the experimental basis for the rational utilization of plant resources and their deep research and development.
引文
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[13]李生茂,刘琳,潘媛,等.川芎挥发油化学成分GC-MS分析及抗氧化活性研究[J].中国民族民间医药,2018,27(2):16-20.
[14]陈勇,杨晨,魏后超,等.磨盘草挥发油化学成分的GC-MS分析[J].中国民族民间医药,2010(3):25-26.
[15]陈光亮,佘玉宝,李冬梅.薄荷油及其有效成分药理作用的研究概况[J].中国中医药信息杂志,2000(2):33-34.
[16]张春娥,张惠,刘楚怡,等.亚油酸的研究进展[J].粮食加工,2010(5):18-21.
[2]广西壮族自治区食品药品监督管理局.广西壮族自治区壮药质量标准(第一卷)[S].南宁:广西科学技术出版社,2008:49.
[3]覃迅云,罗金裕,高志刚.中国瑶药学[M].北京:民族出版社,2002:934.
[4]卢文杰,陆国寿,谭晓,等.壮瑶药小槐花化学成分研究[J].中药材,2013,36(12):1953-1956.
[5]吴瑶,罗强,孙翠玲,等.小槐花的化学成分研究[J].中国中药杂志,2012,37(12):1788-1792.
[6]成林,刘吉成,卢森华,等. HPLC测定小槐花药材中山柰酚的含量[J].中国实验方剂学杂志,2013,19(15):92-94.
[7]陆国寿,谭晓,陈家源,等.小槐花中的脂溶性成分分析[J].广西科学,2012,19(4):355-357.
[8]李燕婧,钟正贤,卢文杰,等.小槐花水提物药理作用研究[J].中医药导报,2013,19(4):76-78.
[9]李燕婧,钟正贤,卢文杰,等.小槐花醇提物药理作用研究[J].云南中医中药杂志,2013,34(5):64-65.
[10]刘超,王俊霞,顾雪竹,等.小槐花提取物对α-葡萄糖苷酶抑制活性研究[J].中国实验方剂学杂志,2013,19(20):91-93.
[11]卢森华,李耀华,陈勇,等.当归藤不同部位挥发油成分GC-MS分析[J].安徽农业科学,2012,40(2):733-735.
[12]李耀华,卢澄生,曾颖虹,等.不同产地葛根挥发油成分的比较分析[J].中国民族民间医药,2014,(11):24-25.
[13]李生茂,刘琳,潘媛,等.川芎挥发油化学成分GC-MS分析及抗氧化活性研究[J].中国民族民间医药,2018,27(2):16-20.
[14]陈勇,杨晨,魏后超,等.磨盘草挥发油化学成分的GC-MS分析[J].中国民族民间医药,2010(3):25-26.
[15]陈光亮,佘玉宝,李冬梅.薄荷油及其有效成分药理作用的研究概况[J].中国中医药信息杂志,2000(2):33-34.
[16]张春娥,张惠,刘楚怡,等.亚油酸的研究进展[J].粮食加工,2010(5):18-21.