广西蔗区检测发现检疫性病害甘蔗白条病
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摘要
为明确2016年在广西北海、来宾、百色蔗区发现的疑似甘蔗白条病症状蔗株致病病原。利用白条黄单胞菌特异性引物XAF1/XAR1对采自这3个蔗区的62份疑似病样进行PCR检测鉴定。鉴定结果表明:62份疑似病样都能够获得约600 bp的特异性片段,所得序列完全一致大小均为608 bp(Gen Bank登录号:KY315183--KY315203),其与白条黄单胞菌rax B1基因核苷酸序列(Gen Bank登录号:FP565176)一致性为100%,在系统进化树中处于同一分支。田间调查结果显示:甘蔗品种‘桂糖46号’、‘桂糖06-2081’对于白条黄单胞菌高度感病,病株率一般为18%~50%,严重田块高达100%;染病严重蔗株全叶枯萎,茎的节部长出许多侧芽,侧芽叶片具白色条纹,造成大幅度减产减糖。本研究在广西蔗区检测发现由白条黄单孢菌引起的检疫性病害甘蔗白条病。
The aim is to identify the pathogen of suspected sugarcane leaf scald plants observed in Beihai,Laibin, and Baise in Guangxi Province in 2016. The PCR detection with the specific primers XAF1/XAR1 of Xanthomonas allbilineans was performed on 62 suspected leaf scald samples collected from the three areas.The identification results showed that the 600 bp specific fragment was amplified in all 62 suspected leaf scald samples, and all nucleotide sequences from this study were identical, 608 bp in length(Gen Bank accession numbers: KY315183-KY315203), shared 100% sequence identity with the nucleotide sequence of rax B1 gene of X. allbilineans(Gen Bank accession number: FP565176), and they clustered in the same branch in the phylogenetic tree. The field surveys showed that sugarcane cultivar ‘Guitang46' and ‘Guitang06-2081' were highly susceptible to leaf scald, the disease incidences were 18%-50%, and even reached 100% in seriously infected fields. The whole leaves blighted in severe infected plants, the node of stem sent forth many lateral buds, and the leaves in lateral buds had white stripes, and sharply caused the reduction of yield and sugar. In this study, we found that sugarcane leaf scald disease was caused by X. albilineans in Guangxi sugarcane region.
The aim is to identify the pathogen of suspected sugarcane leaf scald plants observed in Beihai,Laibin, and Baise in Guangxi Province in 2016. The PCR detection with the specific primers XAF1/XAR1 of Xanthomonas allbilineans was performed on 62 suspected leaf scald samples collected from the three areas.The identification results showed that the 600 bp specific fragment was amplified in all 62 suspected leaf scald samples, and all nucleotide sequences from this study were identical, 608 bp in length(Gen Bank accession numbers: KY315183-KY315203), shared 100% sequence identity with the nucleotide sequence of rax B1 gene of X. allbilineans(Gen Bank accession number: FP565176), and they clustered in the same branch in the phylogenetic tree. The field surveys showed that sugarcane cultivar ‘Guitang46' and ‘Guitang06-2081' were highly susceptible to leaf scald, the disease incidences were 18%-50%, and even reached 100% in seriously infected fields. The whole leaves blighted in severe infected plants, the node of stem sent forth many lateral buds, and the leaves in lateral buds had white stripes, and sharply caused the reduction of yield and sugar. In this study, we found that sugarcane leaf scald disease was caused by X. albilineans in Guangxi sugarcane region.
引文
[1]Rott P,Bailey R A,Comstock J C,et al.A guide to sugarcane diseases[M].Montpellier:CIRAD and ISSCT,2000.
[2]Ricaud C,Ryan C C.Leaf scald:Disease of Sugarcane:Major Disease[M].New York:Elsevier Science Publishing Company Inc,1989.
[3]North D S.Leaf-Scald:A Bacterial Disease of Sugarcane[M].Australia Sydney:Colonial Sugar Refining Company,1926.
[4]Hoy J W,Grisham M P.Sugarcane leaf scald distribution,symptomatology,and effect on yield in Louisiana[J].Plant Disease,1994,78(11):1083-1086.
[5]Rott P,Soupa D,Brunet Y,et al.Leaf scald(Xanthomonas albilineans)incidence and its effect on yield in seven sugarcane cultivars in Guadeloupe[J].Plant Pathology,1995,44(6):1075-1084.
[6]Saumtally A S,Dookun-Saumtally A,Rao G P,et al.Leaf scald of sugarcane:a disease of worldwide importance[M].2004.
[7]Davis M J,Rott P,Warmuth C J et al.Intraspecific genomic variation within Xanthomonas albilineans,the sugarcane leaf scald pathogen[J].Phytopathology,1997(87):316-324.
[8]Rott P,Davis M.Leaf scald.In A guide to sugarcane diseases[M].Montpellier:CIRAD-ISSCT,2000:339.
[9]Klett P,Rott P.Inoculum sources for the spread of leaf scald disease of sugarcane caused by Xanthomonas albilineans in Guadeloupe[J].Journal of Phytopathology,1994,142(3):283-291.
[10]Daugrois J H,Boisne-Noc R,Champoiseau P,et al.The revisited infection cycle of Xanthomonas albilineans,the causal agent of leaf scald of sugarcane[J].Functional Plant Science and Biotechnology,2012,6(2):91-97.
[11]Birch R G.Xanthomonas albilineans and the antipathogenesis approach to disease control[J].Molecular Plant Pathology,2001,2(2):1-11.
[12]李文凤,黄应昆.现代甘蔗病害诊断检测与防控技术[M].北京:中国农业出版社,2012:10.
[13]Flynn J L,Anderlini T A.Disease incidence and yield performance of tissue culture generated seedcane over the crop cycle in Louisiana[J].Journal of American Society of Sugar Cane Technologists,1990(10):113.
[14]Egan B T,Sturgess O W.Commercial control of leaf scald disease by the rmotherapy and a clean seed program[J].Proceedings of the International Society of Sugar Cane Technologists,1980(17):1602-1606.
[15]黄鸿能.广东蔗区甘蔗病害种类和分布的调查及其主要病害的防治[J].甘蔗糖业,1987(5):50-52.
[16]全国甘蔗重要病害研究协作组.中国大陆植蔗省(区)(部分)甘蔗病害种类调查初报[J].甘蔗糖业,1991(1):1-8.
[17]Wang Z K,Comstock J C,Hatziloukas E,et al.Comparison of PCR,BIO-PCR,DIA,ELISA and isolation on semiselective medium for detection of Xanthomonas albilineans,the causal agent of leaf scald of sugarcane[J].Plant Pathology,1999,48(2):245-252.
[18]Comstock J C,Irey M S.Detection of the sugarcane leaf scald pathogen,Xanthomonas albilineans,using tissue blot immunoassay,ELISA,and isolation techniques[J].Plant Disease 1992(76):1033-1035.
[19]Rott P,Davis M,Baudin P.Serological variability in Xanthomonas albilineans,causal agent of leaf scald disease of sugarcane[J].Plant Pathol.1994(43):344-349.
[20]Pan Y B,Grisham M P,Burner D M,et al.Development of polymerase chain reaction primers highly specific for Xanthomonas albilineans,the causal bacterium of sugarcane leaf scald disease[J].Plant Disease,1999(83):218-222.
[21]Tamura K,Stecher G,Peterson D,et al.Molecular evolutionary genetics analysis version 6.0[J].Molecular Biology and Evolution.2013,30(12):2725-2729.
[2]Ricaud C,Ryan C C.Leaf scald:Disease of Sugarcane:Major Disease[M].New York:Elsevier Science Publishing Company Inc,1989.
[3]North D S.Leaf-Scald:A Bacterial Disease of Sugarcane[M].Australia Sydney:Colonial Sugar Refining Company,1926.
[4]Hoy J W,Grisham M P.Sugarcane leaf scald distribution,symptomatology,and effect on yield in Louisiana[J].Plant Disease,1994,78(11):1083-1086.
[5]Rott P,Soupa D,Brunet Y,et al.Leaf scald(Xanthomonas albilineans)incidence and its effect on yield in seven sugarcane cultivars in Guadeloupe[J].Plant Pathology,1995,44(6):1075-1084.
[6]Saumtally A S,Dookun-Saumtally A,Rao G P,et al.Leaf scald of sugarcane:a disease of worldwide importance[M].2004.
[7]Davis M J,Rott P,Warmuth C J et al.Intraspecific genomic variation within Xanthomonas albilineans,the sugarcane leaf scald pathogen[J].Phytopathology,1997(87):316-324.
[8]Rott P,Davis M.Leaf scald.In A guide to sugarcane diseases[M].Montpellier:CIRAD-ISSCT,2000:339.
[9]Klett P,Rott P.Inoculum sources for the spread of leaf scald disease of sugarcane caused by Xanthomonas albilineans in Guadeloupe[J].Journal of Phytopathology,1994,142(3):283-291.
[10]Daugrois J H,Boisne-Noc R,Champoiseau P,et al.The revisited infection cycle of Xanthomonas albilineans,the causal agent of leaf scald of sugarcane[J].Functional Plant Science and Biotechnology,2012,6(2):91-97.
[11]Birch R G.Xanthomonas albilineans and the antipathogenesis approach to disease control[J].Molecular Plant Pathology,2001,2(2):1-11.
[12]李文凤,黄应昆.现代甘蔗病害诊断检测与防控技术[M].北京:中国农业出版社,2012:10.
[13]Flynn J L,Anderlini T A.Disease incidence and yield performance of tissue culture generated seedcane over the crop cycle in Louisiana[J].Journal of American Society of Sugar Cane Technologists,1990(10):113.
[14]Egan B T,Sturgess O W.Commercial control of leaf scald disease by the rmotherapy and a clean seed program[J].Proceedings of the International Society of Sugar Cane Technologists,1980(17):1602-1606.
[15]黄鸿能.广东蔗区甘蔗病害种类和分布的调查及其主要病害的防治[J].甘蔗糖业,1987(5):50-52.
[16]全国甘蔗重要病害研究协作组.中国大陆植蔗省(区)(部分)甘蔗病害种类调查初报[J].甘蔗糖业,1991(1):1-8.
[17]Wang Z K,Comstock J C,Hatziloukas E,et al.Comparison of PCR,BIO-PCR,DIA,ELISA and isolation on semiselective medium for detection of Xanthomonas albilineans,the causal agent of leaf scald of sugarcane[J].Plant Pathology,1999,48(2):245-252.
[18]Comstock J C,Irey M S.Detection of the sugarcane leaf scald pathogen,Xanthomonas albilineans,using tissue blot immunoassay,ELISA,and isolation techniques[J].Plant Disease 1992(76):1033-1035.
[19]Rott P,Davis M,Baudin P.Serological variability in Xanthomonas albilineans,causal agent of leaf scald disease of sugarcane[J].Plant Pathol.1994(43):344-349.
[20]Pan Y B,Grisham M P,Burner D M,et al.Development of polymerase chain reaction primers highly specific for Xanthomonas albilineans,the causal bacterium of sugarcane leaf scald disease[J].Plant Disease,1999(83):218-222.
[21]Tamura K,Stecher G,Peterson D,et al.Molecular evolutionary genetics analysis version 6.0[J].Molecular Biology and Evolution.2013,30(12):2725-2729.