甜茶素和甜茶总多酚的制备
|
摘要
设计了从甜茶叶同时制备甜茶素和甜茶总多酚的工艺,简化了甜茶素生产流程,提高了产率。首先将甜茶提取液用大孔树脂富集,收集柱流液,然后通过低浓度的乙醇溶液对饱和的甜茶素富集柱进行洗脱,合并洗脱液和柱流液,用大孔树脂对合并液进行纯化,得到的总多酚产品纯度可以达到约60%,产率约为10%,再用高浓度的乙醇溶液对甜茶素富集柱进行洗脱,旋干,得到甜茶素粗品,纯度约为45%,产率为7.1%。若再用聚酰胺树脂对甜茶素粗品进行精制,则纯度可达到约71%,产率为3.4%。
Technology of preparing rubusoside and total polyphenols from sweet tea in the same time was designed. The technology simplified the process of preparing rubusoside and improved the yield. First of all sweet tea extract was enriched by the macroporous resin and the column flow fluid was collected. Then the saturated rubusoside enrichment column was eluted by low concentration of ethanol solution,the eluent and column flow fluid were combined and purified by macroporous resin HPD826. Column conduction was flow rate of 2 BV / h,sample volune of 2BV and 40% ethanol solution of 3 BV as eluent. Eluent was collected and concentrated by rotary evaporator and freeze-dried. The purity and yield of the total polyphenols production were 60% and 10%,respectively. Then the enrichment column was eluted again by a high concentration of ethanol solution,eluent was dried by the rotary evaporator,and crude product of rubusoside was obtained. The purity and yield of production were 45% and 7. 1%,respectively. Polyamide resin was used for refining crude product; column condition was same as HPD826. Column flow was collected and concentrated by rotary evaporator and freeze-dried. The purity of production could reach about 71%,the yield was about 3. 4%. The solvent and macroporous resin could be recycled in the whole new process flow; the technological process was simple,green,and suitable for industrial production.
Technology of preparing rubusoside and total polyphenols from sweet tea in the same time was designed. The technology simplified the process of preparing rubusoside and improved the yield. First of all sweet tea extract was enriched by the macroporous resin and the column flow fluid was collected. Then the saturated rubusoside enrichment column was eluted by low concentration of ethanol solution,the eluent and column flow fluid were combined and purified by macroporous resin HPD826. Column conduction was flow rate of 2 BV / h,sample volune of 2BV and 40% ethanol solution of 3 BV as eluent. Eluent was collected and concentrated by rotary evaporator and freeze-dried. The purity and yield of the total polyphenols production were 60% and 10%,respectively. Then the enrichment column was eluted again by a high concentration of ethanol solution,eluent was dried by the rotary evaporator,and crude product of rubusoside was obtained. The purity and yield of production were 45% and 7. 1%,respectively. Polyamide resin was used for refining crude product; column condition was same as HPD826. Column flow was collected and concentrated by rotary evaporator and freeze-dried. The purity of production could reach about 71%,the yield was about 3. 4%. The solvent and macroporous resin could be recycled in the whole new process flow; the technological process was simple,green,and suitable for industrial production.
引文
[1]邓绍林.广西甜茶叶片的营养成分及开发利用价值研究[J].中国林副特产,2000(3):18-19.
[2]周如金,黄敏,张玲,等.大孔树脂提取甜茶苷的研究[J].林产化学与工业,2008,28(5):35-39.
[3]陈全斌,张巧云,义祥辉.高纯度甜茶甙的制备[J].林业科技,2006,31(4):55-56.
[4]吕鑫华,况鹏群,袁其朋,等.制备色谱法在纯化甜茶苷工艺中的应用[J].北京化工大学学报:自然科学版,2012,39(5):92-97.
[5]龙永诚,韦忠孙,马剑霜,等.甜茶原粉生产及产品指标制定[J].企业科技与发展,2014(5):15-16.
[6]葛益银,张涛,张凌云,等.广西甜茶生理活性成分及其提取纯化研究进展[J].食品工业科技,2014,35(4):388-391,396.
[7]滕建文,黄丽,易湘茜,等.广西甜茶多酚的抗过敏性评价[J].食品与机械,2008,24(5):48-51.
[8]张强华,熊清平,石莹莹.大孔树脂对甜菊糖苷溶液的脱色研究[J].食品工业科技,2011,32(5):249-252.
[9]卢昕,张新申,刘承伟.反相高效液相色潽法测定广西甜茶中的甜茶素[J].色谱,2003,21(3):260-262.
[10]崔紫娇,张彩云,刘彬彬,等.Folin-Ciocalteu比色法测定甜茶总多酚含量[J].贵州农业科学,2014,42(3):158-160.
[11]陈全斌,沈钟苏,张巧云,等.甜茶中黄酮甙元的分离提纯及其表征[J].林业科技,2005,30(1):46-48.
[12]葛益银,张凌云.广西甜茶苷柱纯化研究[J].中国野生植物资源,2012,31(5):33-36,40.
[13]吕春茂,宋雨涵,孟宪军,等.大孔树脂纯化寒富苹果渣多酚工艺优化[J].食品工业科技,2012,33(6):301-303,308.
[14]吴应熊,戴静.用大孔吸附树脂分离甜茶甙[J].食品科学,1990(5):12-13.
[2]周如金,黄敏,张玲,等.大孔树脂提取甜茶苷的研究[J].林产化学与工业,2008,28(5):35-39.
[3]陈全斌,张巧云,义祥辉.高纯度甜茶甙的制备[J].林业科技,2006,31(4):55-56.
[4]吕鑫华,况鹏群,袁其朋,等.制备色谱法在纯化甜茶苷工艺中的应用[J].北京化工大学学报:自然科学版,2012,39(5):92-97.
[5]龙永诚,韦忠孙,马剑霜,等.甜茶原粉生产及产品指标制定[J].企业科技与发展,2014(5):15-16.
[6]葛益银,张涛,张凌云,等.广西甜茶生理活性成分及其提取纯化研究进展[J].食品工业科技,2014,35(4):388-391,396.
[7]滕建文,黄丽,易湘茜,等.广西甜茶多酚的抗过敏性评价[J].食品与机械,2008,24(5):48-51.
[8]张强华,熊清平,石莹莹.大孔树脂对甜菊糖苷溶液的脱色研究[J].食品工业科技,2011,32(5):249-252.
[9]卢昕,张新申,刘承伟.反相高效液相色潽法测定广西甜茶中的甜茶素[J].色谱,2003,21(3):260-262.
[10]崔紫娇,张彩云,刘彬彬,等.Folin-Ciocalteu比色法测定甜茶总多酚含量[J].贵州农业科学,2014,42(3):158-160.
[11]陈全斌,沈钟苏,张巧云,等.甜茶中黄酮甙元的分离提纯及其表征[J].林业科技,2005,30(1):46-48.
[12]葛益银,张凌云.广西甜茶苷柱纯化研究[J].中国野生植物资源,2012,31(5):33-36,40.
[13]吕春茂,宋雨涵,孟宪军,等.大孔树脂纯化寒富苹果渣多酚工艺优化[J].食品工业科技,2012,33(6):301-303,308.
[14]吴应熊,戴静.用大孔吸附树脂分离甜茶甙[J].食品科学,1990(5):12-13.