α-葡聚糖酶在甜菜制糖中的试验研究
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摘要
葡聚糖是制糖工业中一种较为常见的危害物,当糖汁中葡聚糖浓度较高时,会影响到过滤、蒸发、结晶和分蜜等工段,而且会增加废蜜的糖分损失、降低提糖率,从而给制糖生产带来严重的负面影响。目前,最有效的解决方案就是使用α-葡聚糖酶,通过酶解作用将葡聚糖降解成小分子糖类,从而解决过滤等生产问题。本文采用单因素分析法研究pH值、温度、反应时间和葡聚糖酶浓度4个因素对葡聚糖的降解效果的影响,确定在甜菜制糖中α-葡聚糖酶去除葡聚糖的最佳应用条件。结果表明,当葡聚糖浓度在1 g/kg以下时,α-葡聚糖酶的最优作用浓度为5 mg/kg,最适反应温度为50～60℃,最优pH值范围为5.0～7.0,有效作用时间为5～10 min。本研究有效解决了工厂葡聚糖浓度升高、中间汁过滤困难的生产难题,保障了低品质甜菜的正常加工,对甜菜糖的生产具有积极的指导意义。
Dextran is a common adverse element in beet sugar industry. High concentration of dextran in sugar juice affects filtration, evaporation, crystallization and purging processes, and increases the loss of sugar and reduces the sugar extraction rate. Thus, it negatively affects the sugar production. At present, the most effective solution is to use α-glucanase to degrade dextran into small molecular carbohydrates by enzymatic hydrolysis, so as to solve the production problems such as filtration. In this study, the effects of pH, temperature, reaction time and concentration of glucanase on the degradation of dextran were studied by single factor analysis, and the optimum conditions for the removal of dextran in production of beet sugar were determined. The results showed that on the condition of lower dextran concentration than 1 g/kg in sugar beet, the optimal condition is that concentration of α-dextranase is 5 mg/kg, the reaction temperature is 50-60 ℃, pH is 5.0-7.0, and the effective time is 5-10 minutes. This study effectively solved the problem of high concentration of dextran in sugar beet production and difficulty in filtering intermediate juice, which guaranteed the normal processing of low quality sugar beet, and has a positive guiding significance for the production of beet sugar.
Dextran is a common adverse element in beet sugar industry. High concentration of dextran in sugar juice affects filtration, evaporation, crystallization and purging processes, and increases the loss of sugar and reduces the sugar extraction rate. Thus, it negatively affects the sugar production. At present, the most effective solution is to use α-glucanase to degrade dextran into small molecular carbohydrates by enzymatic hydrolysis, so as to solve the production problems such as filtration. In this study, the effects of pH, temperature, reaction time and concentration of glucanase on the degradation of dextran were studied by single factor analysis, and the optimum conditions for the removal of dextran in production of beet sugar were determined. The results showed that on the condition of lower dextran concentration than 1 g/kg in sugar beet, the optimal condition is that concentration of α-dextranase is 5 mg/kg, the reaction temperature is 50-60 ℃, pH is 5.0-7.0, and the effective time is 5-10 minutes. This study effectively solved the problem of high concentration of dextran in sugar beet production and difficulty in filtering intermediate juice, which guaranteed the normal processing of low quality sugar beet, and has a positive guiding significance for the production of beet sugar.
引文
[1]贺湘,赵振刚,于淑娟,平野淳也.葡聚糖酶应用于甘蔗混合汁的工艺优化[J].食品工业科技,2012,33(4):330-335.
[2]Eggleston G,Monge A. Optimization of sugarcane factory application of commercial dextranases[J]. Process Biochemistry,2005,40(5):1881-1894.
[3]马步,常国炜,蚁细苗,等.葡聚糖酶在原糖精炼生产中的应用研究[J].广西糖业,2014(3):18-21.
[4]Asadi M. Beet-Sugar Handbook[M]. Wiley-Interscience,2007:173.
[5]梁达奉,曾练强,郭亭,等.葡聚糖对制糖工业的影响及对策(上)[J].甘蔗糖业,2008(3):28-32.
[6]吴兆鹏,梁达奉,曾练强,等.毕赤酵母产α-葡聚糖酶发酵工艺研究[J].粮油食品科技,2010,18(2):29-31.
[7]黄俊杰,范家恒.酶在蔗汁澄清中的应用[J].甘蔗糖业,2008(4):36-57.
[8]Novo Nordisk A/S. Dextranase Novo 25L. A dextran decomposing enzyme for the sugar industry[P]. Product data information 112-GB,Novo Enzyme Division,Dagsvaerd,Denmark,1977.
[9]Cerutti de GD O,Cardenas G,Oliver G. Sucrose utilization and dextran production by Leuconostoc mesenteroides isolated from the sugar industry[J]. Sugar J,2000,62:36-40.
[10]曾练强,王生育,李雨虹,等.葡聚糖多克隆抗体竞争抑制ELISA检测方法的建立[J].轻工科技,2009,25(12):11-12.
[11]戚荣,梁达奉,陈海宁,等.抗葡聚糖单克隆抗体制备的研究[J].甘蔗糖业,2006(3):40-42.
[12]柳颖,蚁细苗,林荣珍,等.免疫比色法定量测定α-葡聚糖的研究[J].广西蔗糖,2012,12(4):17-22.
[13]陆海勤,麻少莹,谢彩锋,等.α-葡聚糖酶在蔗汁澄清工艺中的应用研究[J].食品工业,2012,33(6):46-49.
[14]梁达奉,曾练强,郭亭,等.葡聚糖对制糖工业的影响及对策(下)[J].甘蔗糖业,2008(4):23-27.
[15]柳颖,蚁细苗,林荣珍,等.免疫比色法定量测定葡聚糖的研究[J].广西糖业,2012(4):17-22.
[16]蚁细苗,柳颖,林荣珍,等.免疫比浊法定量测定制糖过程中的葡聚糖[J].广西糖业,2013(4):35-40.
[17]Jiménez E R. Dextranase in sugar industry:A review[J]. Sugar Tech,2009,11(2):124-134.
[18]吴兆鹏,蚁细苗,曾练强,等.α-葡聚糖酶的酶学性质初步研究[J].甘蔗糖业,2012(3):47-52.
[19]岑成福,蚁细苗,梁达奉,等.酶解除去蔗汁中的右旋糖酐[J].甘蔗糖业,2013(6):12-18.
[2]Eggleston G,Monge A. Optimization of sugarcane factory application of commercial dextranases[J]. Process Biochemistry,2005,40(5):1881-1894.
[3]马步,常国炜,蚁细苗,等.葡聚糖酶在原糖精炼生产中的应用研究[J].广西糖业,2014(3):18-21.
[4]Asadi M. Beet-Sugar Handbook[M]. Wiley-Interscience,2007:173.
[5]梁达奉,曾练强,郭亭,等.葡聚糖对制糖工业的影响及对策(上)[J].甘蔗糖业,2008(3):28-32.
[6]吴兆鹏,梁达奉,曾练强,等.毕赤酵母产α-葡聚糖酶发酵工艺研究[J].粮油食品科技,2010,18(2):29-31.
[7]黄俊杰,范家恒.酶在蔗汁澄清中的应用[J].甘蔗糖业,2008(4):36-57.
[8]Novo Nordisk A/S. Dextranase Novo 25L. A dextran decomposing enzyme for the sugar industry[P]. Product data information 112-GB,Novo Enzyme Division,Dagsvaerd,Denmark,1977.
[9]Cerutti de GD O,Cardenas G,Oliver G. Sucrose utilization and dextran production by Leuconostoc mesenteroides isolated from the sugar industry[J]. Sugar J,2000,62:36-40.
[10]曾练强,王生育,李雨虹,等.葡聚糖多克隆抗体竞争抑制ELISA检测方法的建立[J].轻工科技,2009,25(12):11-12.
[11]戚荣,梁达奉,陈海宁,等.抗葡聚糖单克隆抗体制备的研究[J].甘蔗糖业,2006(3):40-42.
[12]柳颖,蚁细苗,林荣珍,等.免疫比色法定量测定α-葡聚糖的研究[J].广西蔗糖,2012,12(4):17-22.
[13]陆海勤,麻少莹,谢彩锋,等.α-葡聚糖酶在蔗汁澄清工艺中的应用研究[J].食品工业,2012,33(6):46-49.
[14]梁达奉,曾练强,郭亭,等.葡聚糖对制糖工业的影响及对策(下)[J].甘蔗糖业,2008(4):23-27.
[15]柳颖,蚁细苗,林荣珍,等.免疫比色法定量测定葡聚糖的研究[J].广西糖业,2012(4):17-22.
[16]蚁细苗,柳颖,林荣珍,等.免疫比浊法定量测定制糖过程中的葡聚糖[J].广西糖业,2013(4):35-40.
[17]Jiménez E R. Dextranase in sugar industry:A review[J]. Sugar Tech,2009,11(2):124-134.
[18]吴兆鹏,蚁细苗,曾练强,等.α-葡聚糖酶的酶学性质初步研究[J].甘蔗糖业,2012(3):47-52.
[19]岑成福,蚁细苗,梁达奉,等.酶解除去蔗汁中的右旋糖酐[J].甘蔗糖业,2013(6):12-18.