蚕丝蛋白短纤维的制备方法
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摘要
通过单因素控制法,在碱性条件下,分别探究温度、质量分数对蚕丝蛋白纤维溶解的影响,通过显微镜观察不同溶解时间的蚕丝蛋白纤维形貌变化,确定其溶解的最佳工艺,并运用扫描电镜、红外光谱检测方法,研究蚕丝蛋白纤维的形貌和结构变化。结果表明:蚕丝蛋白短纤维的最佳制备工艺为温度60～65℃,碱液与尿素之比1︰6,溶解时间40～45 min。在最佳工艺条件下,蚕丝短纤维的丝胶基本已经去除,表面平整光滑,蚕丝蛋白内部结构也没有发生变化。蚕丝短纤维的长度在2～3 mm,比较均匀,制备率40%～50%。
Through single factor control method, the effects of temperature and concentration on fibrinolysis in alkaline conditions were explored. The morphological changes of silk protein fiber in different dissolution time were observed by microscope, and the optimum dissolving process was confirmed. Besides, scanning electron microscope(SEM) and infrared spectroscopy(IR) were used to research the morphological and structural changes of silk protein fiber. The results show that the best preparation conditions for silk protein short fiber are as below: temperature 60-65 ℃, ratio of lye to urea 1︰6 and dissolution time 40-45 min. In the optimum conditions, the sericin of silk short fiber was basically removed, the surface was flat and smooth. The internal structure was not changed. The length of silk short fiber was uniform, ranging from 2 mm to 3 mm, and the preparation rate was 40%-45%.
Through single factor control method, the effects of temperature and concentration on fibrinolysis in alkaline conditions were explored. The morphological changes of silk protein fiber in different dissolution time were observed by microscope, and the optimum dissolving process was confirmed. Besides, scanning electron microscope(SEM) and infrared spectroscopy(IR) were used to research the morphological and structural changes of silk protein fiber. The results show that the best preparation conditions for silk protein short fiber are as below: temperature 60-65 ℃, ratio of lye to urea 1︰6 and dissolution time 40-45 min. In the optimum conditions, the sericin of silk short fiber was basically removed, the surface was flat and smooth. The internal structure was not changed. The length of silk short fiber was uniform, ranging from 2 mm to 3 mm, and the preparation rate was 40%-45%.
引文
[1]沈维治,廖森泰,刘吉平.蚕丝新用途开发的研究进展[J].广东蚕业,2008,42(3):45-48.SHEN Weizhi,LIAO Sentai,LIU Jiping.The research progress of new application of silk [J].Guangdong Sericulture,2008,42(3):45-48.
[2]吴惠英.再生丝素蛋白纤维及其在生物医用材料中的研究进展[J].丝绸,2017,54(3):6-12.WU Huiying.Research progress in regenerated silk fibroin fiber and its application in biomedical materials [J].Journal of Silk,2017,54(3):6-12.
[3]ZUBIR N,PUSHPANATHAN K.Silk in biomedical engineering:a review [J].International Journal of Engineering Inventions,2016,5(8):18-19.
[4]孙安霞,张临康,纪桢,等.蚕丝蛋白防晒霜的制备与评价[J].广东化工,2018,45(18):36-38.SUN Anxia,ZHANG Linkang,JI Zhen,et al.Study on preparation and evaluation of silk protein sunscreen cream [J].Guangdong Chemical Industry,2018,45(18):36-38.
[5]刘凯,匡大江,王诗怡,等.蚕丝蛋白用作胶囊壳材料的研究[J].丝绸,2018,55(12):6-11.LIU Kai,KUANG Dajiang,WANG Shiyi,et al.Study on silk protein used as capsule shell material [J].Journal of Silk,2018,55(12):6-11.
[6]马艳,李智,冉瑞龙,等.蚕丝在生物医用材料领域的应用研究[J].材料导报,2018,32(1):86-90.MA Yan,LI Zhi,RAN Ruilong,et al.Research on application of silk in biomaterial field [J].Materials Reviews,2018,32(1):86-90.
[7]陈永峰,郑德宇,郭闻师.柞蚕丝素蛋白和壳聚糖复合材料的制备及其生物学特性[J].武汉大学学报,2019,40(1):84-89.CHEN Yongfeng,ZHENG Deyu,GUO Wenshi.Preparation of the artificial scaffold formed by tussah silk fibroin/chitosan and its biological properties research [J].Medical Journal of Wuhan University,2019,40(1):84-89.
[8]吴惠英,周燕,左保齐.丝素蛋白纤维人工韧带的制备及性能分析[J].丝绸,2016,53(7):1-6.WU Huiying,ZHOU Yan,ZUO Baoqi.Preparation of artificial ligament with silk fibroin and analysis of its property [J].Journal of Silk,2016,53(7):1-6.
[9]MONDAL M,TRIVEDY K,KUMAR S N.The silk proteins,sericin and fibroin in silkworm,Bombyx mori Linn:a review [J].Caspian Journal of Environmental Sciences,2007,5(2):63-76.
[10]张艳,董照明,席星航,等.家蚕脱胶蚕丝的蛋白组成成分[J].中国农业科学,2018,51(11):2216-2224.ZHANG Yan,DONG Zhaoming,XI Xinghang,et al.Protein components of degumming bombyx mori silk [J].Scientia Agricultura Sinica,2018,51(11):2216-2224.
[11]李玲玲,周伟,代方银,等.不同钙-醇溶解体系丝素蛋白的制备及表征研究[J].中国生物工程杂志,2012,32(4):28-32.LI Lingling,ZHOU Wei,DAI Fangyin,et al.Preparation and characterization of silk fibroin treated with different calcium-alcohol solution [J].China Biotechnology,2012,32(4):28-32.
[12]王鹏,左保齐.不同盐/甲酸溶解体系下丝素膜的制备及性能表征[J].丝绸,2016,53(7):18-22.WANG Peng,ZUO Baoqi.Preparation of silk fibroin films in different salt/formic acid dissolution system and its property characterization [J].Journal of Silk,2016,53(7):18-22.
[13]YUE X X,ZHANG F,WU H Y,et al.A novel route to prepare dry-spun fibers from CaCl2-formic acid solution [J].Materials Letters,2014,128:175-178.
[14]黄雷,王学英,崔霞,等.酶法水解丝素蛋白最佳条件的研究[J].安徽农业科学,2006,34(3):428-429.HUANG Lei,WANG Xueying,CUI Xia,et al.Optimum condition of silk fibroin hydrolysed with enzymes [J].Journal of Anhui Agricultural Sciences,2006,34(3):428-429.
[15]周佳,孙勇,唐传球,等.对二甲氨基苯甲醛比色法测定溶液中的尿素[J].化学与生物工程,2014,31(2):75-77.ZHOU Jia,SUN Yong,TANG Chuanqiu,et al.Determination of urea in solution by colorimetry method of para-dimethyl-amino-benzaldehyde [J].Chemistry & Bioengineering,2014,31(2):75-77.
[16]LUE Ang,LIU Yating,ZHANG Lina,et al.Light scattering study on the dynamic behaviour of cellulose inclusion complex in LiOH/urea aqueous solution [J].Polymer,2011,52:3857-3864.
[17]赵兵,刘家豪,汪涛,等.转基因蚕丝丝素膜的制备与性能表征[J].蚕业科学,2018,44(4):560-566.ZHAO Bing,LIU Jiahao,WANG Tao,et al.Preparation and property characterization of transgenic silk fibroin film [J].Science of Sericulture,2018,44(4):560-566.
[2]吴惠英.再生丝素蛋白纤维及其在生物医用材料中的研究进展[J].丝绸,2017,54(3):6-12.WU Huiying.Research progress in regenerated silk fibroin fiber and its application in biomedical materials [J].Journal of Silk,2017,54(3):6-12.
[3]ZUBIR N,PUSHPANATHAN K.Silk in biomedical engineering:a review [J].International Journal of Engineering Inventions,2016,5(8):18-19.
[4]孙安霞,张临康,纪桢,等.蚕丝蛋白防晒霜的制备与评价[J].广东化工,2018,45(18):36-38.SUN Anxia,ZHANG Linkang,JI Zhen,et al.Study on preparation and evaluation of silk protein sunscreen cream [J].Guangdong Chemical Industry,2018,45(18):36-38.
[5]刘凯,匡大江,王诗怡,等.蚕丝蛋白用作胶囊壳材料的研究[J].丝绸,2018,55(12):6-11.LIU Kai,KUANG Dajiang,WANG Shiyi,et al.Study on silk protein used as capsule shell material [J].Journal of Silk,2018,55(12):6-11.
[6]马艳,李智,冉瑞龙,等.蚕丝在生物医用材料领域的应用研究[J].材料导报,2018,32(1):86-90.MA Yan,LI Zhi,RAN Ruilong,et al.Research on application of silk in biomaterial field [J].Materials Reviews,2018,32(1):86-90.
[7]陈永峰,郑德宇,郭闻师.柞蚕丝素蛋白和壳聚糖复合材料的制备及其生物学特性[J].武汉大学学报,2019,40(1):84-89.CHEN Yongfeng,ZHENG Deyu,GUO Wenshi.Preparation of the artificial scaffold formed by tussah silk fibroin/chitosan and its biological properties research [J].Medical Journal of Wuhan University,2019,40(1):84-89.
[8]吴惠英,周燕,左保齐.丝素蛋白纤维人工韧带的制备及性能分析[J].丝绸,2016,53(7):1-6.WU Huiying,ZHOU Yan,ZUO Baoqi.Preparation of artificial ligament with silk fibroin and analysis of its property [J].Journal of Silk,2016,53(7):1-6.
[9]MONDAL M,TRIVEDY K,KUMAR S N.The silk proteins,sericin and fibroin in silkworm,Bombyx mori Linn:a review [J].Caspian Journal of Environmental Sciences,2007,5(2):63-76.
[10]张艳,董照明,席星航,等.家蚕脱胶蚕丝的蛋白组成成分[J].中国农业科学,2018,51(11):2216-2224.ZHANG Yan,DONG Zhaoming,XI Xinghang,et al.Protein components of degumming bombyx mori silk [J].Scientia Agricultura Sinica,2018,51(11):2216-2224.
[11]李玲玲,周伟,代方银,等.不同钙-醇溶解体系丝素蛋白的制备及表征研究[J].中国生物工程杂志,2012,32(4):28-32.LI Lingling,ZHOU Wei,DAI Fangyin,et al.Preparation and characterization of silk fibroin treated with different calcium-alcohol solution [J].China Biotechnology,2012,32(4):28-32.
[12]王鹏,左保齐.不同盐/甲酸溶解体系下丝素膜的制备及性能表征[J].丝绸,2016,53(7):18-22.WANG Peng,ZUO Baoqi.Preparation of silk fibroin films in different salt/formic acid dissolution system and its property characterization [J].Journal of Silk,2016,53(7):18-22.
[13]YUE X X,ZHANG F,WU H Y,et al.A novel route to prepare dry-spun fibers from CaCl2-formic acid solution [J].Materials Letters,2014,128:175-178.
[14]黄雷,王学英,崔霞,等.酶法水解丝素蛋白最佳条件的研究[J].安徽农业科学,2006,34(3):428-429.HUANG Lei,WANG Xueying,CUI Xia,et al.Optimum condition of silk fibroin hydrolysed with enzymes [J].Journal of Anhui Agricultural Sciences,2006,34(3):428-429.
[15]周佳,孙勇,唐传球,等.对二甲氨基苯甲醛比色法测定溶液中的尿素[J].化学与生物工程,2014,31(2):75-77.ZHOU Jia,SUN Yong,TANG Chuanqiu,et al.Determination of urea in solution by colorimetry method of para-dimethyl-amino-benzaldehyde [J].Chemistry & Bioengineering,2014,31(2):75-77.
[16]LUE Ang,LIU Yating,ZHANG Lina,et al.Light scattering study on the dynamic behaviour of cellulose inclusion complex in LiOH/urea aqueous solution [J].Polymer,2011,52:3857-3864.
[17]赵兵,刘家豪,汪涛,等.转基因蚕丝丝素膜的制备与性能表征[J].蚕业科学,2018,44(4):560-566.ZHAO Bing,LIU Jiahao,WANG Tao,et al.Preparation and property characterization of transgenic silk fibroin film [J].Science of Sericulture,2018,44(4):560-566.