矾根品种‘花毯’组织培养快繁技术的研究
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摘要
为建立矾根组培快繁体系,以矾根品种‘花毯’的花序轴为外植体,本研究对其组培快速繁殖进行了系统研究。结果显示,适宜矾根‘花毯’建立无菌系的接种时间为4月上旬;初代诱导培养基为MS+0.6 mg/L6-BA+0.02 mg/L IBA;继代培养基为MS+0.5 mg/L 6-BA,增殖系数达9.1倍;生根培养基为MS+1.0 mg/L IBA,生根率98.05%;生根苗移栽驯化基质为泥炭土:蛭石体积比3:1,成活率达85.11%。本研究中发现,矾根以MS基础培养基添加1.0 mg/L和2.0 mg/L IBA的生根培养基中,根长、根数和生根时间差异均不显著。这说明矾根自身分化能力较强,生根培养所需外源激素较低,从节约成本角度考虑,添加一定量的MS培养基可作为‘花毯’组培苗最佳生根培养基。本研究为建立矾根组培快繁体系提供理论依据。
In order to establish a rapid propagation system for Heuchera micrantha,, the inflorescence axis of the cultivar 'Tapestry' was used as an explant. This study systematically studied the rapid propagation of its tissue culture and rapid propagation. The results showed that the inoculation time for the establishment of a sterile line for Heuchera micrantha 'Tapestry' was in early April. The initial induction medium was MS +0.6 mg/L 6-BA +0.02 mg/L IBA. The subculture medium was MS +0.5 mg/L 6-BA, and the proliferation coefficient reached by 9.1 times. Rooting medium was MS +1.0 mg/L IBA, with the rooting rate of 98.05%. Rooting shoots transplanted and domesticated matrix peat soil: vermiculite volume ratio 3:1, and the survival rate was 85.11%. In this study, the root length, number of roots, and rooting time of roots in 1.0 mg/L IBA and 2.0 mg/L supplemented with MS basic medium were not significantly different. This indicated that Heuchera micrantha had a strong ability to differentiate itself, and the exogenous hormones need for rooting culture was relatively low. From the perspective of cost saving, adding a certain amount of MS medium could be used as the best rooting medium for the 'Tapestry' tissue culture seedlings. This study could provide a theoretical basis for the establishment of a tissue culture and rapid propagation system of Heuchera micrantha.
In order to establish a rapid propagation system for Heuchera micrantha,, the inflorescence axis of the cultivar 'Tapestry' was used as an explant. This study systematically studied the rapid propagation of its tissue culture and rapid propagation. The results showed that the inoculation time for the establishment of a sterile line for Heuchera micrantha 'Tapestry' was in early April. The initial induction medium was MS +0.6 mg/L 6-BA +0.02 mg/L IBA. The subculture medium was MS +0.5 mg/L 6-BA, and the proliferation coefficient reached by 9.1 times. Rooting medium was MS +1.0 mg/L IBA, with the rooting rate of 98.05%. Rooting shoots transplanted and domesticated matrix peat soil: vermiculite volume ratio 3:1, and the survival rate was 85.11%. In this study, the root length, number of roots, and rooting time of roots in 1.0 mg/L IBA and 2.0 mg/L supplemented with MS basic medium were not significantly different. This indicated that Heuchera micrantha had a strong ability to differentiate itself, and the exogenous hormones need for rooting culture was relatively low. From the perspective of cost saving, adding a certain amount of MS medium could be used as the best rooting medium for the 'Tapestry' tissue culture seedlings. This study could provide a theoretical basis for the establishment of a tissue culture and rapid propagation system of Heuchera micrantha.
引文
Chen H.,Tang Y.,Shi Y.H.,and Wu J.,2011,Tissue culture and rapid propagation of Heuchera micrantha,Shanghai Nongye Xuebao(Acta Agriculturae Shanghai),27(4):80-82(陈宏,唐莹,施月欢,吴杰,2011,矾根的组织培养与快速繁殖,上海农业学报,27(4):80-82)
Huang S.L.,Wang H.Q.,Zhou Y.F.,Leng J.M.,and Liu M.,2016,Study on adaptability of Heuchera micrantha in summer during introduction and domestication in Dongguan,Redai Linye(Tropical Forestry),44(4):4-7(黄少玲,汪华清,周意峰,冷江明,刘黾,2016,矾根在东莞地区引种驯化过程中的夏季适应性研究,热带林业,44(4):4-7)
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Sun G.F.,Zhang J.Z.,and Wu D.Q.,2007,Tissue culture and rapid propagation of Heuchera hybrid hort.'Prince of Silver',Zhiwu Shenglixue Tongxun(Plant Physiology Communications),43(3):500(孙国峰,张金政,吴东启,2007,矾根杂种‘银王子’的组织培养和快速繁殖,植物生理学通讯,43(3):500)
Wang J.,Liu L.G.,Zuo L.J.,and Tang C.L.,2012,Study on tissue culture and rapid propagation of Heuchera micrantha,Beifang Yuanyi(Northern Horticulture),(23):116-118(王晶,刘立功,左丽娟,唐存莲,2012,柔毛矾根组培快繁技术研究,北方园艺,(23):116-118)
Zhao X.S.,Li M.Y.,Zhang W.L.,and Liu F.,2009,Establishment of high adventitious shoot regeneration system of ornamental kale,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),28(1):141-148(赵秀枢,李名扬,张文玲,刘凡,2009,观赏羽衣甘蓝高频再生体系的建立,基因组学与应用生物学,28(1):141-148)
Huang S.L.,Wang H.Q.,Zhou Y.F.,Leng J.M.,and Liu M.,2016,Study on adaptability of Heuchera micrantha in summer during introduction and domestication in Dongguan,Redai Linye(Tropical Forestry),44(4):4-7(黄少玲,汪华清,周意峰,冷江明,刘黾,2016,矾根在东莞地区引种驯化过程中的夏季适应性研究,热带林业,44(4):4-7)
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Sun G.F.,Zhang J.Z.,and Wu D.Q.,2007,Tissue culture and rapid propagation of Heuchera hybrid hort.'Prince of Silver',Zhiwu Shenglixue Tongxun(Plant Physiology Communications),43(3):500(孙国峰,张金政,吴东启,2007,矾根杂种‘银王子’的组织培养和快速繁殖,植物生理学通讯,43(3):500)
Wang J.,Liu L.G.,Zuo L.J.,and Tang C.L.,2012,Study on tissue culture and rapid propagation of Heuchera micrantha,Beifang Yuanyi(Northern Horticulture),(23):116-118(王晶,刘立功,左丽娟,唐存莲,2012,柔毛矾根组培快繁技术研究,北方园艺,(23):116-118)
Zhao X.S.,Li M.Y.,Zhang W.L.,and Liu F.,2009,Establishment of high adventitious shoot regeneration system of ornamental kale,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),28(1):141-148(赵秀枢,李名扬,张文玲,刘凡,2009,观赏羽衣甘蓝高频再生体系的建立,基因组学与应用生物学,28(1):141-148)