影响胚胎种植率关键因素的研究进展
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摘要
体外受精-胚胎移植(in-vitro fertilization-embryo transfer,IVF-ET)技术是目前解决不孕症最为有效的治疗方式之一,但胚胎种植率偏低仍是困扰临床医生们的难题之一。影响胚胎种植率因素包括两方面,即来自胚胎的胚胎质量因素和来自母体的子宫内膜容受性因素。现结合近年国内外研究对挑选优质胚胎的新方法,如缩时摄影技术和植入前遗传学筛查,以及子宫内膜容受性和内膜种植窗的相关研究进展进行综述。
In-vitro fertilization-embryo transfer(IVF-ET) is one of the most effective therapies for human infertility. However, the low implantation rate is still one of the clinical problems. The factors related to the rate of embryo implantation are from two aspects, factors of embryo quality and factors of endometrial receptivity. In this review, we discuss the new methods of selecting high-quality embryos such as time-lapse and pre-implantation genetic screening, as well as the endometrial receptivity and window of implantation.
In-vitro fertilization-embryo transfer(IVF-ET) is one of the most effective therapies for human infertility. However, the low implantation rate is still one of the clinical problems. The factors related to the rate of embryo implantation are from two aspects, factors of embryo quality and factors of endometrial receptivity. In this review, we discuss the new methods of selecting high-quality embryos such as time-lapse and pre-implantation genetic screening, as well as the endometrial receptivity and window of implantation.
引文
[1]Achache H,Revel A.Endometrial receptivity markers,the journey to successful embryo implantation[J].Hum Reprod Update,2006,12(6):731-746.
[2]Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology.The Istanbul consensus workshop on embryo assessment:proceedings of an expert meeting[J].Hum Reprod,2011,26(6):1270-1283.
[3]Payne D,Flaherty SP,Barry MF,et al.Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography[J].Hum Reprod,1997,12(3):532-541.
[4]Leung AS,Son WY,Dahan MH.Time-lapse imaging of embryos:current evidence supporting its use[J].Expert Rev Med Devices,2016,13(10):881-883.
[5]Wu YG,Lazzaroni-Tealdi E,Wang Q,et al.Different effectiveness of closed embryo culture system with time-lapse imaging(EmbryoScope(TM))in comparison to standard manual embryology in good and poor prognosis patients:a prospectively randomized pilot study[J].Reprod Biol Endocrinol,2016,14(1):49.
[6]Chen M,Wei S,Hu J,et al.Does time-lapse imaging have favorable results for embryo incubation and selection compared with conventional methods in clinical in vitro fertilization?A metaanalysis and systematic review of randomized controlled trials[J].PLoS One,2017,12(6):e0178720.
[7]Armstrong S,Bhide P,Jordan V,et al.Time-lapse systems for embryo incubation and assessment in assisted reproduction[J].Cochrane Database Syst Rev,2018,5:CD011320.
[8]Reignier A,Lammers J,Barriere P,et al.Can time-lapse parameters predict embryo ploidy?A systematic review[J].Reprod Biomed Online,2018,36(4):380-387.
[9]徐艳文,黄国宁,孙海翔,等.高通量基因测序植入前胚胎遗传学诊断和筛查技术规范(试行)[J].生殖医学杂志,2017,26(5):391-398.
[10]Harton GL,Magli MC,Lundin K,et al.ESHRE PGD Consortium/Embryology Special Interest Group--best practice guidelines for polar body and embryo biopsy for preimplantation genetic diagnosis/screening(PGD/PGS)[J].Hum Reprod,2011,26(1):41-46.
[11]Gleicher N,Kushnir VA,Barad DH.Preimplantation genetic screening(PGS)still in search of a clinical application:a systematic review[J].Reprod Biol Endocrinol,2014,12:22.
[12]张宁媛,黄国宁,范立青,等.胚胎植入前遗传学诊断与筛查实验室技术指南[J].生殖医学杂志,2018,29(9):819-826.
[13]Martín J,Cervero A,Mir P,et al.The impact of next-generation sequencing technology on preimplantation genetic diagnosis and screening[J].Fertil Steril,2013,99(4):1054-1061.e3.
[14]Harper J,Wells D,Simpson JL.Current controversies in prenatal diagnosis 4:preimplantation genetic screening should be routinely offered to all preimplantation genetic diagnosis cases[J].Prenat Diagn,2016,36(1):25-28.
[15]Wang N,Zheng YM,Li L,et al.Preimplantation genetic screening:an effective testing for infertile and repeated miscarriage patients?[J].Obstet Gynecol Int,2010,2010:120130.
[16]Lee HL,McCulloh DH,Hodes-Wertz B,et al.In vitro fertilization with preimplantation genetic screening improves implantation and live birth in women age 40 through 43[J].J Assist Reprod Genet,2015,32(3):435-444.
[17]Murugappan G,Ohno MS,Lathi RB.Cost-effectiveness analysis of preimplantation genetic screening and in vitro fertilization versus expectant management in patients with unexplained recurrent pregnancy loss[J].Fertil Steril,2015,103(5):1215-1220.
[18]Kushnir VA,Darmon SK,Albertini DF,et al.Effectiveness of in vitro fertilization with preimplantation genetic screening:a reanalysis of United States assisted reproductive technology data2011-2012[J].Fertil Steril,2016,106(1):75-79.
[19]Fiorentino F,Bono S,Biricik A,et al.Application of nextgeneration sequencing technology for comprehensive aneuploidy screening of blastocysts in clinical preimplantation genetic screening cycles[J].Hum Reprod,2014,29(12):2802-2813.
[20]Huang J,Yan L,Lu S,et al.Validation of a next-generation sequencing-based protocol for 24-chromosome aneuploidy screening of blastocysts[J].Fertil Steril,2016,105(6):1532-1536.
[21]Zimmerman RS,Tao X,Marin D,et al.Preclinical validation of a targeted next generation sequencing-based comprehensive chromosome screening methodology in human blastocyst[J].Mol Hum Reprod,2018,24(1):37-45.
[22]Xu J,Fang R,Chen L,et al.Noninvasive chromosome screening of human embryos by genome sequencing of embryo culture medium for in vitro fertilization[J].Proc Natl Acad Sci U S A,2016,113(42):11907-11912.
[23]Shamonki MI,Jin H,Haimowitz Z,et al.Proof of concept:preimplantation genetic screening without embryo biopsy through analysis of cell-free DNA in spent embryo culture media[J].Fertil Steril,2016,106(6):1312-1318.
[24]Borini A,Tarozzi N,Lagalla C.Preimplantation genetic diagnosis:why are obstetric and neonatal risks increased?The need for more data[J].Fertil Steril,2016,106(6):1328-1329.
[25]Galliano D,Bellver J,Díaz-García C,et al.ART and uterine pathology:how relevant is the maternal side for implantation?[J].Hum Reprod Update,2015,21(1):13-38.
[26]Noyes RW,Hertig AT,Rock J.Dating the endometrial biopsy[J].Fertil Steril,1950,1(1):3-25.
[27]Quinn C,Ryan E,Claessens EA,et al.The presence of pinopodes in the human endometrium does not delineate the implantation window[J].Fertil Steril,2007,87(5):1015-1021.
[28]Simon C,Sakkas D,Gardner DK,et al.Biomarkers in reproductive medicine:the quest for new answers[J].Hum Reprod Update,2015,21(6):695-697.
[29]Ruan YC,Chen H,Chan HC.Ion channels in the endometrium:regulation of endometrial receptivity and embryo implantation[J].Hum Reprod Update,2014,20(4):517-529.
[30]Horcajadas JA,Riesewijk A,Polman J,et al.Effect of controlled ovarian hyperstimulation in IVF on endometrial gene expression profiles[J].Mol Hum Reprod,2005,11(3):195-205.
[31]Garrido-Gómez T,Ruiz-Alonso M,Blesa D,et al.Profiling the gene signature of endometrial receptivity:clinical results[J].Fertil Steril,2013,99(4):1078-1085.
[32]Gómez E,Ruíz-Alonso M,Miravet J,et al.Human Endometrial Transcriptomics:Implications for Embryonic Implantation[J].Cold Spring Harb Perspect Med,2015,5(7):a022996.
[33]Hashimoto T,Koizumi M,Doshida M,et al.Efficacy of the endometrial receptivity array for repeated implantation failure in Japan:A retrospective,two-centers study[J].Reprod Med Biol,2017,16(3):290-296.
[34]Tan J,Kan A,Hitkari J,et al.The role of the endometrial receptivity array(ERA)in patients who have failed euploid embryo transfers[J].J Assist Reprod Genet,2018,35(4):683-692.
[35]Nejat EJ,Ruiz-Alonso M,Simón C,et al.Timing the window of implantation by nucleolar channel system prevalence matches the accuracy of the endometrial receptivity array[J].Fertil Steril,2014,102(5):1477-1481.
[36]Hu S,Yao G,Wang Y,et al.Transcriptomic changes during the pre-receptive to receptive transition in human endometrium detected by RNA-Seq[J].J Clin Endocrinol Metab,2014,99(12):E2744-E2753.
[37]Zhao G,Cao Y,Zhu X,et al.Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulatingΔNp63expression in Asherman's syndrome[J].Sci China Life Sci,2017,60(4):404-416.
[38]Ding L,Yan G,Wang B,et al.Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POFpatients with long history of infertility[J].Sci China Life Sci,2018,61(12):1554-1565.
[39]陈怡李,姚书忠.CRISPR/Cas9基因编辑技术的应用研究进展[J].国际生殖健康/计划生育杂志,2017,36(6):482-487.
[2]Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology.The Istanbul consensus workshop on embryo assessment:proceedings of an expert meeting[J].Hum Reprod,2011,26(6):1270-1283.
[3]Payne D,Flaherty SP,Barry MF,et al.Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography[J].Hum Reprod,1997,12(3):532-541.
[4]Leung AS,Son WY,Dahan MH.Time-lapse imaging of embryos:current evidence supporting its use[J].Expert Rev Med Devices,2016,13(10):881-883.
[5]Wu YG,Lazzaroni-Tealdi E,Wang Q,et al.Different effectiveness of closed embryo culture system with time-lapse imaging(EmbryoScope(TM))in comparison to standard manual embryology in good and poor prognosis patients:a prospectively randomized pilot study[J].Reprod Biol Endocrinol,2016,14(1):49.
[6]Chen M,Wei S,Hu J,et al.Does time-lapse imaging have favorable results for embryo incubation and selection compared with conventional methods in clinical in vitro fertilization?A metaanalysis and systematic review of randomized controlled trials[J].PLoS One,2017,12(6):e0178720.
[7]Armstrong S,Bhide P,Jordan V,et al.Time-lapse systems for embryo incubation and assessment in assisted reproduction[J].Cochrane Database Syst Rev,2018,5:CD011320.
[8]Reignier A,Lammers J,Barriere P,et al.Can time-lapse parameters predict embryo ploidy?A systematic review[J].Reprod Biomed Online,2018,36(4):380-387.
[9]徐艳文,黄国宁,孙海翔,等.高通量基因测序植入前胚胎遗传学诊断和筛查技术规范(试行)[J].生殖医学杂志,2017,26(5):391-398.
[10]Harton GL,Magli MC,Lundin K,et al.ESHRE PGD Consortium/Embryology Special Interest Group--best practice guidelines for polar body and embryo biopsy for preimplantation genetic diagnosis/screening(PGD/PGS)[J].Hum Reprod,2011,26(1):41-46.
[11]Gleicher N,Kushnir VA,Barad DH.Preimplantation genetic screening(PGS)still in search of a clinical application:a systematic review[J].Reprod Biol Endocrinol,2014,12:22.
[12]张宁媛,黄国宁,范立青,等.胚胎植入前遗传学诊断与筛查实验室技术指南[J].生殖医学杂志,2018,29(9):819-826.
[13]Martín J,Cervero A,Mir P,et al.The impact of next-generation sequencing technology on preimplantation genetic diagnosis and screening[J].Fertil Steril,2013,99(4):1054-1061.e3.
[14]Harper J,Wells D,Simpson JL.Current controversies in prenatal diagnosis 4:preimplantation genetic screening should be routinely offered to all preimplantation genetic diagnosis cases[J].Prenat Diagn,2016,36(1):25-28.
[15]Wang N,Zheng YM,Li L,et al.Preimplantation genetic screening:an effective testing for infertile and repeated miscarriage patients?[J].Obstet Gynecol Int,2010,2010:120130.
[16]Lee HL,McCulloh DH,Hodes-Wertz B,et al.In vitro fertilization with preimplantation genetic screening improves implantation and live birth in women age 40 through 43[J].J Assist Reprod Genet,2015,32(3):435-444.
[17]Murugappan G,Ohno MS,Lathi RB.Cost-effectiveness analysis of preimplantation genetic screening and in vitro fertilization versus expectant management in patients with unexplained recurrent pregnancy loss[J].Fertil Steril,2015,103(5):1215-1220.
[18]Kushnir VA,Darmon SK,Albertini DF,et al.Effectiveness of in vitro fertilization with preimplantation genetic screening:a reanalysis of United States assisted reproductive technology data2011-2012[J].Fertil Steril,2016,106(1):75-79.
[19]Fiorentino F,Bono S,Biricik A,et al.Application of nextgeneration sequencing technology for comprehensive aneuploidy screening of blastocysts in clinical preimplantation genetic screening cycles[J].Hum Reprod,2014,29(12):2802-2813.
[20]Huang J,Yan L,Lu S,et al.Validation of a next-generation sequencing-based protocol for 24-chromosome aneuploidy screening of blastocysts[J].Fertil Steril,2016,105(6):1532-1536.
[21]Zimmerman RS,Tao X,Marin D,et al.Preclinical validation of a targeted next generation sequencing-based comprehensive chromosome screening methodology in human blastocyst[J].Mol Hum Reprod,2018,24(1):37-45.
[22]Xu J,Fang R,Chen L,et al.Noninvasive chromosome screening of human embryos by genome sequencing of embryo culture medium for in vitro fertilization[J].Proc Natl Acad Sci U S A,2016,113(42):11907-11912.
[23]Shamonki MI,Jin H,Haimowitz Z,et al.Proof of concept:preimplantation genetic screening without embryo biopsy through analysis of cell-free DNA in spent embryo culture media[J].Fertil Steril,2016,106(6):1312-1318.
[24]Borini A,Tarozzi N,Lagalla C.Preimplantation genetic diagnosis:why are obstetric and neonatal risks increased?The need for more data[J].Fertil Steril,2016,106(6):1328-1329.
[25]Galliano D,Bellver J,Díaz-García C,et al.ART and uterine pathology:how relevant is the maternal side for implantation?[J].Hum Reprod Update,2015,21(1):13-38.
[26]Noyes RW,Hertig AT,Rock J.Dating the endometrial biopsy[J].Fertil Steril,1950,1(1):3-25.
[27]Quinn C,Ryan E,Claessens EA,et al.The presence of pinopodes in the human endometrium does not delineate the implantation window[J].Fertil Steril,2007,87(5):1015-1021.
[28]Simon C,Sakkas D,Gardner DK,et al.Biomarkers in reproductive medicine:the quest for new answers[J].Hum Reprod Update,2015,21(6):695-697.
[29]Ruan YC,Chen H,Chan HC.Ion channels in the endometrium:regulation of endometrial receptivity and embryo implantation[J].Hum Reprod Update,2014,20(4):517-529.
[30]Horcajadas JA,Riesewijk A,Polman J,et al.Effect of controlled ovarian hyperstimulation in IVF on endometrial gene expression profiles[J].Mol Hum Reprod,2005,11(3):195-205.
[31]Garrido-Gómez T,Ruiz-Alonso M,Blesa D,et al.Profiling the gene signature of endometrial receptivity:clinical results[J].Fertil Steril,2013,99(4):1078-1085.
[32]Gómez E,Ruíz-Alonso M,Miravet J,et al.Human Endometrial Transcriptomics:Implications for Embryonic Implantation[J].Cold Spring Harb Perspect Med,2015,5(7):a022996.
[33]Hashimoto T,Koizumi M,Doshida M,et al.Efficacy of the endometrial receptivity array for repeated implantation failure in Japan:A retrospective,two-centers study[J].Reprod Med Biol,2017,16(3):290-296.
[34]Tan J,Kan A,Hitkari J,et al.The role of the endometrial receptivity array(ERA)in patients who have failed euploid embryo transfers[J].J Assist Reprod Genet,2018,35(4):683-692.
[35]Nejat EJ,Ruiz-Alonso M,Simón C,et al.Timing the window of implantation by nucleolar channel system prevalence matches the accuracy of the endometrial receptivity array[J].Fertil Steril,2014,102(5):1477-1481.
[36]Hu S,Yao G,Wang Y,et al.Transcriptomic changes during the pre-receptive to receptive transition in human endometrium detected by RNA-Seq[J].J Clin Endocrinol Metab,2014,99(12):E2744-E2753.
[37]Zhao G,Cao Y,Zhu X,et al.Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulatingΔNp63expression in Asherman's syndrome[J].Sci China Life Sci,2017,60(4):404-416.
[38]Ding L,Yan G,Wang B,et al.Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POFpatients with long history of infertility[J].Sci China Life Sci,2018,61(12):1554-1565.
[39]陈怡李,姚书忠.CRISPR/Cas9基因编辑技术的应用研究进展[J].国际生殖健康/计划生育杂志,2017,36(6):482-487.