固氮菌分离及其荚膜染色综合性实验
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摘要
采用直接分离法和梯度稀释法分离固氮菌,利用该中心改良的染色方法观察固氮菌的荚膜。通过综合性实验让学生掌握微生物分离技术和荚膜染色方法,并认识固氮球菌的形态特征,提升学生动手能力,为培养学生的创新思维奠定基础。
Azotobacter is not easy to be preserved,and it needs to be separated before it is used.Azotobacter is separated by the direct separation method and gradient dilution method,and the capsule of azotobacter is observed by the improved staining method in this center.Through the comprehensive experiment,students can master the microbial separation technology and capsule staining method,and understand the morphological characteristics of azotobacter,which improves students' practical ability,and lays the foundation for training their innovative thinking.
Azotobacter is not easy to be preserved,and it needs to be separated before it is used.Azotobacter is separated by the direct separation method and gradient dilution method,and the capsule of azotobacter is observed by the improved staining method in this center.Through the comprehensive experiment,students can master the microbial separation technology and capsule staining method,and understand the morphological characteristics of azotobacter,which improves students' practical ability,and lays the foundation for training their innovative thinking.
引文
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[10]余阳,吴小倩,杨静,等.环境微生物实验教学体系改革和管理[J].实验技术与管理,2016,33(4):194-197.
[11]魏建宏,罗琳.微生物学综合性设计实验教学中存在的问题与探索[J].微生物生物学通报,2017,44(1):225-231.
[12]王文涛,赵宏,刘荣娟,等.大学生创新实验室建设研究[J].实验技术与管理,2016,33(11):30-36.
[2]韩斌,孔继君,邹晓明,等.生物固氮研究现状及展望[J].山西农业科学,2009,37(10):86-89.
[3]罗明,卢云.植物内生固氮菌研究进展[J].微生物学杂志,2005,25(1):82-88.
[4]Burris R H.Biological nitrogen fixation[J].Plant Physiology,1974,54(4):443-449.
[5]赵琳.生物固氮与生态农业[J].江西农业学报,2006,18(5):166-168.
[6]刘支梅.圆褐固氮菌荚膜染色法改进的研究[J].华中师范大学学报,1988.22(1):83-85.
[7]沈萍,陈向东.微生物学实验[M].北京:高等教育出版社,2007.
[8]朱铁群,陈庆利.微生物学设计性实验教学个案分析[J].微生物学通报,2007,34(5):32-33.
[9]谢明杰,宋岚,汪业菊,等.微生物综合设计性实验的组织与实施[J].实验技术与管理,2010,27(11):137-138.
[10]余阳,吴小倩,杨静,等.环境微生物实验教学体系改革和管理[J].实验技术与管理,2016,33(4):194-197.
[11]魏建宏,罗琳.微生物学综合性设计实验教学中存在的问题与探索[J].微生物生物学通报,2017,44(1):225-231.
[12]王文涛,赵宏,刘荣娟,等.大学生创新实验室建设研究[J].实验技术与管理,2016,33(11):30-36.