High Efficiency Hypocrellin Production by a Novel Mutant Isolated from Shiraia bambusicola
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摘要
The fungus Shiraia bambusicola GZ19 M1 is biotechnologically important due to its ability to biosynthesis the pigment hypocrellins.Results showed that ethyl methane sulfonate( EMS) was effective mutagenic agent for strain and potentially produced large numbers of random mutations broadly and uniformly over the whole genome to generate unique strains. Wild-type cultures of S. bambusicola GZ19 were subjected to EMS( 80 mM) induction targeted at approximately 20% spores' survival. When surviving spores were selected in sufficient numbers and cultured on PDA medium for 7 d at 26℃,five novel mutagenized S. bambusicola strains were obtained. A mutant GZ19 M1 that exhibited an activity of more than two times over the wild strain was obtained. Also,batch experiments were carried out to achieve the suitable conditions for hypocrellin. Glucose and rice extract were the most favorable carbon and nitrogen sources for hypocrellin production by submerged culture of S. bambusicola GZ19 M1,and initial glucose and rice extract concentrations were at 35 and 250 g/L,respectively. The optimal surfactant were found to be 0. 008 V/V Tween 80,it was added into cultivation medium at 24 h. Hypocrellin concentration reached 498. 89 mg/L under optimal nutritional conditions,an increment of about 8. 70 times of hypocrellins production was observed compared with that of in non-optimized medium by the wild strain.
The fungus Shiraia bambusicola GZ19 M1 is biotechnologically important due to its ability to biosynthesis the pigment hypocrellins.Results showed that ethyl methane sulfonate( EMS) was effective mutagenic agent for strain and potentially produced large numbers of random mutations broadly and uniformly over the whole genome to generate unique strains. Wild-type cultures of S. bambusicola GZ19 were subjected to EMS( 80 mM) induction targeted at approximately 20% spores' survival. When surviving spores were selected in sufficient numbers and cultured on PDA medium for 7 d at 26℃,five novel mutagenized S. bambusicola strains were obtained. A mutant GZ19 M1 that exhibited an activity of more than two times over the wild strain was obtained. Also,batch experiments were carried out to achieve the suitable conditions for hypocrellin. Glucose and rice extract were the most favorable carbon and nitrogen sources for hypocrellin production by submerged culture of S. bambusicola GZ19 M1,and initial glucose and rice extract concentrations were at 35 and 250 g/L,respectively. The optimal surfactant were found to be 0. 008 V/V Tween 80,it was added into cultivation medium at 24 h. Hypocrellin concentration reached 498. 89 mg/L under optimal nutritional conditions,an increment of about 8. 70 times of hypocrellins production was observed compared with that of in non-optimized medium by the wild strain.
The fungus Shiraia bambusicola GZ19 M1 is biotechnologically important due to its ability to biosynthesis the pigment hypocrellins.Results showed that ethyl methane sulfonate( EMS) was effective mutagenic agent for strain and potentially produced large numbers of random mutations broadly and uniformly over the whole genome to generate unique strains. Wild-type cultures of S. bambusicola GZ19 were subjected to EMS( 80 mM) induction targeted at approximately 20% spores' survival. When surviving spores were selected in sufficient numbers and cultured on PDA medium for 7 d at 26℃,five novel mutagenized S. bambusicola strains were obtained. A mutant GZ19 M1 that exhibited an activity of more than two times over the wild strain was obtained. Also,batch experiments were carried out to achieve the suitable conditions for hypocrellin. Glucose and rice extract were the most favorable carbon and nitrogen sources for hypocrellin production by submerged culture of S. bambusicola GZ19 M1,and initial glucose and rice extract concentrations were at 35 and 250 g/L,respectively. The optimal surfactant were found to be 0. 008 V/V Tween 80,it was added into cultivation medium at 24 h. Hypocrellin concentration reached 498. 89 mg/L under optimal nutritional conditions,an increment of about 8. 70 times of hypocrellins production was observed compared with that of in non-optimized medium by the wild strain.
引文
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[13]REITEN M,MALACHIN G,KOMMISRUD E,et al.Stress resilience of spermatozoa and blood mononuclear cells without prion protein[J].Frontiers in Molecular Biosciences,2018,5(4):123-125.
[14]GHARIBZAHEDI S,RAZAVI S,MOUSAVI S,et al.High efficiency canthaxanthin production by a novel mutant isolated from Dietzia natronolimnaea HS-1 using central composite design analysis[J].Industrial Crops and Products,2012,40(13):345-354.
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[16]LI K,LU H,HANG F,et al.Improved dextranase production by Chaetomium gracile through optimization of carbon source and fermentation parameters[J].Sugar Tech,2017,19(4):432-437.
[17]KOKKINIDOU S,PETERSON D,BLOCH T,et al.The important role of carbohydrates in the flavor,function,and formulation of oral nutritional supplements[J].Nutrients,2018,10(6):742.
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[20]AJIBADE A.Effects of different types of surfactants functionalized on the surface of carbon nanotubes on the mechanical&thermal properties of natural rubber/CNT nano composite[D].Saudi Arabia:King Fahd University,2010.
[2]DU W,SUN C,LIANG Z,et al.Antibacterial activity of hypocrellin Aagainst Staphylococcus Aureus[J].World Journal of Microbiology and Biotechnology,2012,28(4):3151-3157.
[3]JI YY,MA YJ,WANG JW.Cytoprotective role of nitric oxide in Hep G2cell apoptosis induced by hypocrellin B photodynamic treatment[J].Journal of Photochemistry&Photobiology,B:Biology,2016,163(5):366-373.
[4]HUANG M,ZHAO P,XIONG M,et al.Antidiabetic activity of perylenequinonoid-rich extracts from Shiraia bambusicola in KK-Ay mice with spontaneous type 2 diabetes mellitus[J].Journal of Ethnopharmacology,2016,191(12):71-81.
[5]SAVERGAVE LS,GADRE RV,VAIDYA BK,et al.Strain improvement and statistical media optimization for enhanced erythritol production with minimal by-products from Candida magnoliae mutant R23[J].Biochemical Engineering Journal,2011,55(2):92-100.
[6]NAVEENA B,GOPINATH KP,SAKTHISELVAN P,et al.Enhanced production of thrombinase by Streptomyces venezuelae:kinetic studies on growth and enzyme production of mutant strain[J].Bioresource Technology,2012,111(2):417-424.
[7]DU W.Study on selection of high hypocrellin producing strain and antimicrobial mechanism against Ralstonia solanacearum[D].Guiyang:Guizhou University,2013,12(7):20-40.(in Chinese)
[8]LEI XY,ZHANG MY,MA YJ,et al.Transcriptomic responses involved in enhanced production of hypocrellin A by addition of Triton X-100 in submerged cultures of Shiraia bambusicola[J].Journal of Industrial Microbiology Biotechnology,2017,25(4):126-134.
[9]SUN CX,MA YJ,WANG JW.Enhanced production of hypocrellin A by ultrasound stimulation in submerged cultures of Shiraia bambusicola[J].Ultrasonics Sonochemistry,2017,38(4):214-224.
[10]SUN CX,MA YJ,WANG JW.Improved hypocrellin A production in Shiraia bambusicola by light-dark shift[J].Journal of Photochemistry&Photobiology,B:Biology,2018,182(5):100-107.
[11]MARESMA BG,CASTILLO BG,FERNANDEZ RC,et al.Mutagenesis of Aspergillus oryzae IPT-301 to improve the production ofβ-fructofuranosidase[J].Brazilian Journal of Microbiology,2010,41(21):186-195.
[12]YANG H,XIAO C,MA W,et al.The production of hypocrellin colorants by submerged cultivation of the medicinal fungus Shiraia bambusicola[J].Dyes and Pigments,2009,82(14):142-146.
[13]REITEN M,MALACHIN G,KOMMISRUD E,et al.Stress resilience of spermatozoa and blood mononuclear cells without prion protein[J].Frontiers in Molecular Biosciences,2018,5(4):123-125.
[14]GHARIBZAHEDI S,RAZAVI S,MOUSAVI S,et al.High efficiency canthaxanthin production by a novel mutant isolated from Dietzia natronolimnaea HS-1 using central composite design analysis[J].Industrial Crops and Products,2012,40(13):345-354.
[15]LIU Z,ZHANG K,LIN J,et al.Breeding cold tolerance strain by chemical mutagenesis in Volvariella volvacea[J].Scientia Horticulturae,2011,130(1):18-24.
[16]LI K,LU H,HANG F,et al.Improved dextranase production by Chaetomium gracile through optimization of carbon source and fermentation parameters[J].Sugar Tech,2017,19(4):432-437.
[17]KOKKINIDOU S,PETERSON D,BLOCH T,et al.The important role of carbohydrates in the flavor,function,and formulation of oral nutritional supplements[J].Nutrients,2018,10(6):742.
[18]RIPA F,NIKKON F,ZAMAN S,et al.Optimal conditions for antimicrobial metabolites production from a new Streptomyces sp.RUPA-08PRisolated from Bangladeshi soil[J].Mycobiology,2009,37(12):211-214.
[19]DEKKER R,BARBOSA A,GIESE E,et al.Influence of nutrients on enhancing laccase production by Botryosphaeria rhodina MAMB-05[J].International Microbiology,2007,10(3):177-185.
[20]AJIBADE A.Effects of different types of surfactants functionalized on the surface of carbon nanotubes on the mechanical&thermal properties of natural rubber/CNT nano composite[D].Saudi Arabia:King Fahd University,2010.