青海省不同生态区野生金露梅叶生物活性成分分析和评价
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摘要
本研究以青海省境内不同生态区的金露梅叶为研究对象,分析其生物活性成分含量以及差异。结果表明:乐都县金露梅叶中总氨基酸(11.95%)、总黄酮(18.88 mg/g)、多酚(10.6%)、维生素C (5.25 mg/100 g)、儿茶素(1.16%)、单宁(13.60%)和芦丁(0.077%)的含量最高,蛋白质含量以玉树金露梅叶中最高,达到16.9%;而总氨基酸(7.91%)、总黄酮(12.13%)、茶多酚(6.0%)、维生素C(4.17 mg/100 g)、儿茶素(0.20%)和芦丁(0.0129%)的含量在湟中县金露梅叶中最低。通过方差分析可知,不同地区金露梅叶成分含量都存在着显著差异(p <0.05)。主成分分析筛选出4个指标(总黄酮、儿茶素、维生素C、蛋白质)代表金露梅叶的品质。金露梅叶的生物活性成分含量较高,值得进一步深入研究和开发。
In this study,Potentilla fruticosa leaves in different ecological areas in Qinghai province were selected as the research target.The contents and difference of bioactive components were analyzed and evaluated.The experimental results indicated that total amino acids( 11.95%),total flavonoids( 18.88 mg/g),polyphenols( 10.6%),vitamin C( 5.25 mg/100 g),catechin( 1.16%),tannin( 13.60%) and rutin( 0.0773%) were the highest in the Potentilla fruticosa leaves in Ledu county,Qinghai province.The contents of protein was the highest in the Potentilla fruticosa leaves in yushu county,which reached 16.9%.The total amino acid( 7.91%),total flavonoids( 12.13%),tea polyphenols( 6%),vitamin C( 4.17 mg/100 g),catechin( 0.20%)and rutin( 0.0129%) the contents of Potentilla fruticosa leaves was lowest in Huangzhong county of Qinghai province.According to the analysis of variance,there were significant differences in the contents of components in the Potentilla fruticosa leaves in different areas( p < 0.05).Four indexes( total flavonoids,catechins,vitamin C,protein) were selected by principal component analysis to represents the quality of the Potentilla fruticosa leaves.The contents of bioactive components in leaves of Potentilla fruticosa was high,which would deserve further research and development.
In this study,Potentilla fruticosa leaves in different ecological areas in Qinghai province were selected as the research target.The contents and difference of bioactive components were analyzed and evaluated.The experimental results indicated that total amino acids( 11.95%),total flavonoids( 18.88 mg/g),polyphenols( 10.6%),vitamin C( 5.25 mg/100 g),catechin( 1.16%),tannin( 13.60%) and rutin( 0.0773%) were the highest in the Potentilla fruticosa leaves in Ledu county,Qinghai province.The contents of protein was the highest in the Potentilla fruticosa leaves in yushu county,which reached 16.9%.The total amino acid( 7.91%),total flavonoids( 12.13%),tea polyphenols( 6%),vitamin C( 4.17 mg/100 g),catechin( 0.20%)and rutin( 0.0129%) the contents of Potentilla fruticosa leaves was lowest in Huangzhong county of Qinghai province.According to the analysis of variance,there were significant differences in the contents of components in the Potentilla fruticosa leaves in different areas( p < 0.05).Four indexes( total flavonoids,catechins,vitamin C,protein) were selected by principal component analysis to represents the quality of the Potentilla fruticosa leaves.The contents of bioactive components in leaves of Potentilla fruticosa was high,which would deserve further research and development.
引文
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[13]崔志杰,何玲,刘仲华,等.儿茶素的生物学活性及其应用前景概述[J].动物营养学报,2011,23(10):1664-1668.
[14]张亮亮,汪咏梅,徐曼,等.植物单宁化学结构分析方法研究进展[J].林产化学与工业,2012,32(3):107-116.
[15]王玉增,刘彦.植物单宁研究进展综述[J].西部皮革,2014,36(16):23-30.
[2]杨永昌.藏药志[M].西宁:青海人民出版社,1991.
[3]Shkel N M,Tril V M,Volkhonskaya T A.Method of preparing tea substitute from Potentilla fruticosa.Russia,2061383[P].1996-06-10.
[4]白吉庆,王小平,王媚,等.药王茶总黄酮含量测定分析[J].陕西农业科学,2012,2:18-19.
[5]周卫龙,徐建峰,许凌.GB/T 8313-2008茶叶中茶多酚和儿茶素类含量的测定方法[S].北京:中国标准出版社,2008:1-3.
[6]许晓洁,王伟,王玉华.蒙药材金露梅的化学成分研究[J].天然产物研究与开发,2013,25(10):1367-1369.
[7]李海朝,孙慧珍,胡旭.不同生境金露梅叶中黄酮总量的变化及其变因分析[J].西部林业科学,2007,36(3):71-73.
[8]汤一.茶叶吸香和持香机理的探讨[J].茶叶,2000,26(3):132-135.
[9]邬新荣,王岳飞,张士康,等.茶多酚保健功能研究进展与保健食品开发[J].茶叶科学,2010,30(增刊1):501-505.
[10]高海荣,黄振旭,李华敏.16种中国茶叶中茶多酚含量对比研究[J].食品研究与开发,2016,37(7):33-36.
[11]刘丰华,郭惠娟,王莉.维生素C的药理与临床应用[J].亚太传统医药.2010,6(3):89-90.
[12]贾林艳,宋伟新,庄志萍,等.高效液相色谱法测定茶叶中水溶性维生素的含量[J].中国林副特产,2003,65(2):36-37.
[13]崔志杰,何玲,刘仲华,等.儿茶素的生物学活性及其应用前景概述[J].动物营养学报,2011,23(10):1664-1668.
[14]张亮亮,汪咏梅,徐曼,等.植物单宁化学结构分析方法研究进展[J].林产化学与工业,2012,32(3):107-116.
[15]王玉增,刘彦.植物单宁研究进展综述[J].西部皮革,2014,36(16):23-30.