生物电化学系统还原CO_2合成有机化合物
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摘要
采用生物电化学系统固定CO_2生产有机物。构建三电极反应体系,从活性污泥中筛分出一种电化学活性菌种,和电化学结合还原CO_2,合成乙酸和丙三醇。在阴极电势-0.8 V,12 h时乙酸累积浓度9.2 mmol/L,9 h时丙三醇累积浓度最大为3.5 mmol/L,总库伦效率最大为84%。电化学分析表明,菌种具有良好的活性,确定菌种通过电极和氢气作为介体两种方式传递电子。ITS rRNA鉴定菌种为假丝酵母,并命名为Candida sp.S。研究表明,生物阴极在电能的驱动下,可以将CO_2还原为多种有机物。
Bioelectrochemical systems is used for producing organic chemicals from carbon dioxide. The three-electrode reaction system was constructed,and an electrochemical active microorganism was separated and screened from the activated sludge combine with electrochemistry to synthesize acetic acid and glycerol by reduction of CO_2. At cathode potential of-0. 8 V,the maximum of accumulation for acetic acid reached at 12 h was 9. 2 mmol/L,the cumulative concentration of glycerol was 3. 5 mmol/L at 9 h. The total coulomb efficiency reached 84%. The electrochemical analysis of the bacteria showed its excellent catalytic activity,and determined that the bacteria passed the electrons in two ways through the electrode and the hydrogen as mediator. ITS rRNA identified strains as Candida and named Candida sp. S. The result show that CO_2 can be reduced to multiple organic compounds under the driving of electric energy.
Bioelectrochemical systems is used for producing organic chemicals from carbon dioxide. The three-electrode reaction system was constructed,and an electrochemical active microorganism was separated and screened from the activated sludge combine with electrochemistry to synthesize acetic acid and glycerol by reduction of CO_2. At cathode potential of-0. 8 V,the maximum of accumulation for acetic acid reached at 12 h was 9. 2 mmol/L,the cumulative concentration of glycerol was 3. 5 mmol/L at 9 h. The total coulomb efficiency reached 84%. The electrochemical analysis of the bacteria showed its excellent catalytic activity,and determined that the bacteria passed the electrons in two ways through the electrode and the hydrogen as mediator. ITS rRNA identified strains as Candida and named Candida sp. S. The result show that CO_2 can be reduced to multiple organic compounds under the driving of electric energy.
引文
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[14]Nevin K P,Hensley S A,Franks A E,et al.Electrosynthesis of organic compounds from carbon dioxide is catalyzed by a diversity of acetogenic microorganisms[J].Applied and Environmental Microbiology,2011,77(9):2882-2886.
[2]Clauwaert P.Combining biocatalyzed electrolysis with anaerobic digestion[J].Water Science Technology,2008,57(4):575-579.
[3]Clauwaert P,Van der H.Open air biocathode enables effective electricity generation with microbial fuel cells[J].Enviromental Science&Technology,2007,41(21):7564-7569.
[4]Strycharz S M,Glaven R H,Coppi M V,et al.Gene expression and deletion analysis of mechanisms for electron transfer from electrodes to Geobacter sulfurreducens[J].Bioelectrochemistry,2011,80(2):142-150.
[5]Nie H,Zhang T,Cui M,et al.Improved cathode for high efficient microbial-catalyzed reduction in microbial electrosynthesis cells[J].Physical Chemistry Chemical Physics,2013,15(34):14290-14294.
[6]Han Li,Paul H,Opgenorth,et al.Integrated electromicrobial conversion of CO2to higher alcohols[J].Science,2012,335(3):1596.
[7]Cheng S A,Xing D F,Call D F,et al.Direct biological conversion of electrical current into methane by electromenthanogenesis[J].Environmental Science&Technology,2009,43(10):3953-3958.
[8]Embley T M,Stackebrandt E.The molecular phylogeny and systematics of the actinomycetes[J].Annual Reviews in Microbiology,1994,48(1):257-289.
[9]Rabaey K,Rozendal R A.Microbial electrosynthesis——revisiting the electrical route for microbial production[J].Nature Review Microbial,2010(8):706-716.
[10]Scott K,Murano C.Microbial fuel cells utilizing carbohydrates[J].Jorunal of Chemical Technology and Biotechnology,2007,82(1):92-100.
[11]Van Eerten-Jansen M C A A,Veldhoen A B,Plugge C M,et al.Microbial community analysis of a methane-producing biocathode in a bioelectrochemical system[J].Archaea,2013(9):481784-481796.
[12]Muller V.Energy conservation in acetogenic bacteria[J].Apply Environment Microbiol,2003,69(11):6345-6353.
[13]Nevin K P,Woodard T L,Franks A E,et al.Microbial electrosynthesis:feeding microbes electricity to convert carbon dioxide and water to multicarbon extracellular organic compounds[J].Micro Biology,2010,1(2):e00103-10.
[14]Nevin K P,Hensley S A,Franks A E,et al.Electrosynthesis of organic compounds from carbon dioxide is catalyzed by a diversity of acetogenic microorganisms[J].Applied and Environmental Microbiology,2011,77(9):2882-2886.