不同剂量维生素D_3对大鼠肾毒性的影响
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摘要
目的:探讨不同剂量维生素D_3(Vitamin D_3,VD_3)对大鼠肾毒性的影响。方法:将健康SD大鼠100只随机分为正常对照组和VD_3四个不同剂量组,各20只。对照组生理盐水灌胃,VD_3低、中、高、超高剂量组VD_3溶液灌胃,剂量分别为20 IU/kg、80 IU/kg、200 IU/kg、800 IU/kg,连续灌胃6周,观察大鼠体质量变化,检测血清尿素氮(BUN)、肌酐(CRE)及Ca~(2+)含量,观察肾组织病理切片变化。结果:各剂量组雄性大鼠体质量均大于对照组,差异有统计学意义(P <0.05);VD_3超大剂量组血清Ca~(2+)含量高于对照组,差异有统计学意义(P <0.01);病理切片显示:VD_3各剂量组光镜下观察均没有明显改变。结论:800 IU/kg可能是引起VD_3中毒的初始剂量,对临床使用VD_3有重要的参考和指导意义。
Objective: To explore the effect of different doses of vitamin D_3(vitamin D_3, VD_3) on nephrotoxicity in rats. Methods: 100 healthy SD rats were randomly divided into a normal control group and four VD_3 dose groups, 20 each. The rats in the control group were given normal saline by lavage. The rats in VD_3 low, medium, high, ultra-high dose groups were given VD_3 solution by lavage, and the doses were 20 IU/kg, 80 IU/kg, 200 IU/kg, 800 IU/kg, respectively. During continuous lavage for 6 weeks, the changes in body weight of rats were observed, serum urea nitrogen(BUN), creatinine(CRE) and Ca~(2+) contents were detected, and the changes in pathological sections of renal tissues were observed. Results: The body weight of male rats in each dose group was higher than that in the control group, and the difference was statistically significant(P<0.05). The content of serum Ca~(2+) in the VD_3 super-high dose group was higher than that in the control group, and the difference was statistically significant(P<0.01). The pathological sections showed that there were no significant changes in light microscopy in each dose group of VD_3. Conclusion: 800 IU/kg may be the initial dose of VD_3 poisoning, which has an important reference and guiding significance for the clinical use of VD_3.
Objective: To explore the effect of different doses of vitamin D_3(vitamin D_3, VD_3) on nephrotoxicity in rats. Methods: 100 healthy SD rats were randomly divided into a normal control group and four VD_3 dose groups, 20 each. The rats in the control group were given normal saline by lavage. The rats in VD_3 low, medium, high, ultra-high dose groups were given VD_3 solution by lavage, and the doses were 20 IU/kg, 80 IU/kg, 200 IU/kg, 800 IU/kg, respectively. During continuous lavage for 6 weeks, the changes in body weight of rats were observed, serum urea nitrogen(BUN), creatinine(CRE) and Ca~(2+) contents were detected, and the changes in pathological sections of renal tissues were observed. Results: The body weight of male rats in each dose group was higher than that in the control group, and the difference was statistically significant(P<0.05). The content of serum Ca~(2+) in the VD_3 super-high dose group was higher than that in the control group, and the difference was statistically significant(P<0.01). The pathological sections showed that there were no significant changes in light microscopy in each dose group of VD_3. Conclusion: 800 IU/kg may be the initial dose of VD_3 poisoning, which has an important reference and guiding significance for the clinical use of VD_3.
引文
[1]窦晓娟,汪求真,韩孝红,等.大剂量维生素D3补充对糖尿病大鼠淋巴细胞增殖活性的影响[J].中国药理学通报,2016,32(4):589-590.
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[7]杨文彬,李百强,史向华,等.维生素D3低剂量连续给药对大鼠肾脏超微结构的变化[J].山西医药杂志,2017,46(3):271-273.
[2]Nebbioso M,Buomprisco G,Pascarella A,et al.Modulatory Effects of 1,25-dihydroxyvitamin D3 on Eye Disorders:A Critical Review[J].Crit Rev Food Sci Nutr,2017,57(3):559-565.
[3]谢顺英,罗炎姣.儿童营养性维生素D缺乏性佝偻病合并缺铁性贫血的临床分析[J].中国药物经济学,2015,10(S1):238-239.
[4]朱庆龄,盛晓阳.维生素D过量及中毒的研究进展[J].中华儿科杂志,2015,53(3):235-237.
[5]1Alshahrani F,Aljohani N.Vitamin D:deficiency,sufficiency and toxicity[J].Nutrients,2013,5(9):3605-3616.
[6]Vieth R.Why the optimal requirement for Vitamin D3 is probably much higher than what is officially recommended for adults[J].J Steroid Biochem Mol Biol,2004,89-90(1-5):575-579.
[7]杨文彬,李百强,史向华,等.维生素D3低剂量连续给药对大鼠肾脏超微结构的变化[J].山西医药杂志,2017,46(3):271-273.