生脉饮(党参方)中5-羟甲基糠醛的分离及其来源探究
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摘要
为探讨生脉饮(党参方)的物质基础及配伍规律,采用大孔树脂富集结合中压柱色谱纯化,从生脉饮(党参方)中分离得到5-羟甲基糠醛.通过进一步的拆方研究发现:在含五味子的合煎组中,5-羟甲基糠醛的量均较单煎组有明显提高.推测生脉饮(党参方)中麦冬及/或党参与五味子的合煎过程可能会产生5-羟甲基糠醛.首次从生脉饮(党参方)中分离鉴定出5-羟甲基糠醛,同时提供了一种从中药复方中快速分离5-羟甲基糠醛的方法,并对生脉饮(党参方)的配伍规律进行了初步探索.
To investigate the material base and compatibility law of Shengmaiyin(Dangshenfang),macroporous resin column chromatography combined with medium-pressure chromatography were applied to separate the chemical constituents from Shengmaiyin(Dangshenfang).As a result,a kind of constituents was isolated and identified as 5-hydroxymethylfurfural through1 HNMR in the present study. Furthermore,the origin of 5-hydroxymethylfurfural was investigated by decomposing recipes of Shengmaiyin(Dangshenfang).The result showed that the content of 5-hydroxymethylfurfural in single drug was much lower than con-decocting of two or three drugs which contained Schisandrae Fructus.It can be concluded that the con-decocting procedure of Codonopsis Radix and/or Ophiopogonis Radix with Schisandrae Fructus may produced 5-hydroxymethylfurfural.Our study presented the first report on isolation of 5-hydroxymethylfurfural from Shengmaiyin(Dangshenfang).In addition,a rapid and effective method was developed to separate 5-hydroxymethylfurfural from Chinese medicinal formula was established.Moreover,the compatibility law of Shengmaiyin(Dangshenfang)was explored.
To investigate the material base and compatibility law of Shengmaiyin(Dangshenfang),macroporous resin column chromatography combined with medium-pressure chromatography were applied to separate the chemical constituents from Shengmaiyin(Dangshenfang).As a result,a kind of constituents was isolated and identified as 5-hydroxymethylfurfural through1 HNMR in the present study. Furthermore,the origin of 5-hydroxymethylfurfural was investigated by decomposing recipes of Shengmaiyin(Dangshenfang).The result showed that the content of 5-hydroxymethylfurfural in single drug was much lower than con-decocting of two or three drugs which contained Schisandrae Fructus.It can be concluded that the con-decocting procedure of Codonopsis Radix and/or Ophiopogonis Radix with Schisandrae Fructus may produced 5-hydroxymethylfurfural.Our study presented the first report on isolation of 5-hydroxymethylfurfural from Shengmaiyin(Dangshenfang).In addition,a rapid and effective method was developed to separate 5-hydroxymethylfurfural from Chinese medicinal formula was established.Moreover,the compatibility law of Shengmaiyin(Dangshenfang)was explored.
引文
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[14]ZHANG Lei,ZHU Lin,WANG Yuefei,et al.Characterization and quantification of major constituents of Xue Fu Zhu Yu by UPLC-DAD-MS/MS[J].Journal of Agricultural and Food Chemistry,2012,62:203-209.
[15]刘月新,潘雪,邹茜.探讨5-羟甲基糠醛在中药及复方中的存在意义[J].中国现代药物应用,2013,7(15):3-5.
[16]张俊姣,张阳,蒋晓燕,等.糖基原料热解形成5-羟甲基糠醛的特性与机理综述[J].新能源进展,2014,2(1):7-12.
[17]刘昕.高温液态水在有机酸对果糖分解反应动力学的影响[D].杭州:浙江大学,2005.
[2]黄青萍.生脉饮中人参与党参的薄层鉴别[J].广西中医药,2004,27(1):57.
[3]沈烈行.生脉饮药理作用与临床应用[J].医药导报,2003,22(9):634-635.
[4]CHEN Fangfang,QI Huanyang,SHI Yanping,et al.Fingerprint analysis of Codonopsis radix by HPLC coupled with chemometrics analysis[J].Chinese Herbal Medicines,2013,5(4):307-312.
[5]王进,刘汉清,刘霖,等.HPLC定量指纹图谱法评价川麦冬质量研究[J].中药材,2013,36(5):721-725.
[6]KIM Y J,LEE H J,KIM C Y,et al.Simultaneous determination of nine lignans from Schisandra chinensis extract using ultra-performance liquid chromatography with tandem mass spectrometry in rat plasma,urine,and gastrointestinal tract samples:application to the pharmacokinetic study of Schisandra chinensis[J].Journal of Separation Science,2014,37(20):2851-2863.
[7]刘月新,杜芳麓.5-羟甲基糠醛的合成工艺研究[J].中国现代药物应用,2010,4(1):3-5.
[8]楚楚,徐绍静,吴琼琼,等.HPLC同时测定生脉饮(党参方)中多组分的含量[J].中药材,2013,36(5):820-823.
[9]ABRHAM K,GURTLER R,BERG K,et al.Toxicology and risk assessment of 5-hydroxymethylfurfural in food[J].Molecular Nutrition and Food Research,2011,55(5):667-678.
[10]张玉玉,宋弋,李全宏,等.食品中糠醛和5-羟甲基糠醛的产生机理、含量检测及安全性评价研究进展[J].食品科学,2012,33(5):275-280.
[11]ZHAO Ling,CHEN Jianping,SU Jianyu,et al.In vitro antioxidant and antiproliferative activities of 5-hydroxymethylfurfural[J].Journal of Agricultural and Food Chemistry,2013,61(44):10604-10611.
[12]NAESSBERGER L.Influence of 5-hydroxymethylfurfural(5-HMF)on the overall metabolism of human blood cells[J].Human and Experimental Toxicology,1990,9(4):211-214.
[13]朱丹妮,李志明,严永清,等.生脉散复方化学动态变化与药效关系的研究——生脉散复方化学的研究(Ⅱ)[J].中国中药杂志,1998,23(5):291-293.
[14]ZHANG Lei,ZHU Lin,WANG Yuefei,et al.Characterization and quantification of major constituents of Xue Fu Zhu Yu by UPLC-DAD-MS/MS[J].Journal of Agricultural and Food Chemistry,2012,62:203-209.
[15]刘月新,潘雪,邹茜.探讨5-羟甲基糠醛在中药及复方中的存在意义[J].中国现代药物应用,2013,7(15):3-5.
[16]张俊姣,张阳,蒋晓燕,等.糖基原料热解形成5-羟甲基糠醛的特性与机理综述[J].新能源进展,2014,2(1):7-12.
[17]刘昕.高温液态水在有机酸对果糖分解反应动力学的影响[D].杭州:浙江大学,2005.