摘要
有害中波紫外线(Ultraviolet B, UV-B; 280~320 nm)辐射影响植物的生长和发育, Ca~(2+)是植物生长发育所必须的大量元素之一,外源Ca~(2+)不但可以提高植物抗胁迫能力,还对次生代谢具有调控作用。以颠茄(AtropabelladonnaL.)实生苗为材料,在UV-B辐射(10μW cm~(–2))背景下,研究不同浓度外源Ca~(2+)、不同处理时间对颠茄生理特性、氮代谢、次生代谢产物含量以及托品烷类生物碱代谢途径中3个关键酶基因表达量的影响。结果表明,随着UV-B辐射时间的延长(4~12 d),对颠茄的光合作用、氮代谢以及生物碱的积累产生抑制作用,加剧了膜脂氧化程度;经外源Ca~(2+)处理,叶片初始荧光(Fo)、丙二醛(malondialdehyde,MDA)含量呈下降趋势,叶片最大光化学效率(F_v/F_m)、光合色素(总叶绿素、类胡萝卜素)含量、抗氧化酶(SOD、POD、CAT)活性均呈上升趋势,说明Ca~(2+)有利于缓解UV-B辐射的抑制,加强对UV-B胁迫的抗性;在Ca~(2+)处理下,叶片中硝态氮含量显著降低,游离氨基酸、可溶性蛋白含量和氮代谢关键酶(NR、GS、GDH)的活性显著提高,叶片中莨菪碱含量和东莨菪碱含量显著提高;qRT-PCR分析显示,在外源Ca~(2+)的诱导下,托品烷类生物碱合成途径中3个关键酶基因(PMT、TR I、H6H)表达量均有不同程度上调趋势。本研究结果可为田间种植提供理论参考。
Ultraviolet B(UV-B) radiation does harm to plant, for which growth and development. Ca~(2+) is one of the most necessary elements. Exogenous Ca~(2+) can enhance plant abilities to resist stress and regulate secondary metabolism. In this research, we used Atropa belladonna L. seedlings to investigate the effects of different concentrations of exogenous Ca~(2+) and different treatment days on the physiological characteristics, nitrogen metabolism, secondary metabolites content and relative genes expression levels of three key enzymes in secondary metabolism under the background of UV-B radiation. The UV-B radiation not only caused inhibitory effect on the Atropa belladonna L. photosynthesis, nitrogen metabolism, alkaloid content, but also increased peroxidation of membrane lipid. The Fo, MDA content gradually decreased, but the F_v/F_m, photosynthetic pigment content, antioxidant enzyme activity gradually increased under Ca~(2+) treatment, showing that Ca~(2+) is propitious to relieve inhibitory effect on photosynthesis and enhance resistance to UV-B stress. Moreover, Ca~(2+) reduced the content of nitrate nitrogen significantly, promoted the contents of free amino acids, soluble proteins, atropine alkaloids, and raised the activities of key enzymes in nitrogen metabolism. Using real-time fluorescence quantitative PCR, we found that exogenous Ca~(2+) increased the relative gene expression levels of three key enzymes in secondary metabolism in different degrees. This research could provide a theoretical references for plantation in the field.
引文
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