氮素形态对紫花苜蓿不同生育期生长特性的影响
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摘要
在完全营养液条件下,采用砂培法,研究了3种氮素形态配比(NO-3-N,NH+4-N以及NO-3-N∶NH+4-N为1∶1)和3个氮素水平(0、105、210mg/L)对‘甘农3号’紫花苜蓿整个生育期生长特性的影响.结果表明:不同形态氮素处理下紫花苜蓿的株高、地上生物量、地下生物量、根瘤数、根瘤质量均显著高于CK,表现为:NO-3-N和NH+4-N混合培养下效果最好,NH+4-N培养下次之,NO-3-N培养下最低.随着氮素水平的增加,各形态配比下紫花苜蓿的株高、地上生物量、地下生物量、根瘤数、根瘤质量均呈现增加的变化趋势.紫花苜蓿的株高、地上生物量、地下生物量、根瘤数、根瘤质量均在NO-3-N+NH+4-N的质量浓度为210mg/L时,达到最大值.各处理下紫花苜蓿的各指标均在苗期、现蕾期、盛花期差异比较明显,结荚期和鼓粒期差异不显著.整个生育期,紫花苜蓿的各生长指标之间有不同程度的相关性.
Under the condition of complete nutrient solution,the cultivation of the whole growth period by sand culture,the effects of three kinds of nitrogen forms(nitrate nitrogen,ammonium nitrogen and nitrate nitrogen,ammonium nitrogen is 1∶1)and three nitrogen levels(0,105 and 210mg/L)were studied on growth characteristics of alfalfa‘Gannong No.3'at different growth stages.The results showed that different nitrogen treatments had significantly higher on plant height,aboveground biomass,underground biomass,nodule number and nodule weight than those of CK,NO-3-N and NH+4-N mixed culture showed the best,then NH+4-N training,and NO-3-N training was the lowest.Along with the increase of nitrogen level,various forms of plant height,aboveground biomass,underground biomass,nodule number,nodule weight showed a trend of increasing.The plant height,aboveground biomass,underground biomass,nodule number,root nodule weight reached a maximum in NO-3-N NH+4-N when the concentration at 210mg/L.Theindexes of plant growth under different treatments showed significantly different in seedling,budding,flowering periods;however,not significant different was found in seed podding and podding periods.It is suggested that the growth index of alfalfa have different degree of correlation.
Under the condition of complete nutrient solution,the cultivation of the whole growth period by sand culture,the effects of three kinds of nitrogen forms(nitrate nitrogen,ammonium nitrogen and nitrate nitrogen,ammonium nitrogen is 1∶1)and three nitrogen levels(0,105 and 210mg/L)were studied on growth characteristics of alfalfa‘Gannong No.3'at different growth stages.The results showed that different nitrogen treatments had significantly higher on plant height,aboveground biomass,underground biomass,nodule number and nodule weight than those of CK,NO-3-N and NH+4-N mixed culture showed the best,then NH+4-N training,and NO-3-N training was the lowest.Along with the increase of nitrogen level,various forms of plant height,aboveground biomass,underground biomass,nodule number,nodule weight showed a trend of increasing.The plant height,aboveground biomass,underground biomass,nodule number,root nodule weight reached a maximum in NO-3-N NH+4-N when the concentration at 210mg/L.Theindexes of plant growth under different treatments showed significantly different in seedling,budding,flowering periods;however,not significant different was found in seed podding and podding periods.It is suggested that the growth index of alfalfa have different degree of correlation.
引文
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[6]俞艳,刘晓静,齐敏兴,等.pH和氮素形态对紫花苜蓿根瘤特性的影响[J].草原与草坪,2012,32(5):7-11
[7]Duxbury J M,Cherney J H.Inorganic nitrogen supply and symbiotic dinitro-gen fixation in alfalfa[J].Journal of Plant Nutrition,1994,17(12):2053-2067
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[10]孙建光,张燕春,徐晶,等.高效固氮芽孢杆菌筛选及其生物学特性[J].中国农业科学,2009,42(6):2043-2051
[11]蒯佳林,刘晓静,李文卿.不同氮素水平对接种根瘤菌紫花苜蓿生长特性的影响[J].草原与草坪,2010,31(3):56-59
[12]蒋名川,解淑贞.蔬菜施肥[M].北京:农业出版社,1985
[13]曹翠玲,李生秀.氮素形态对作物生理特性及生长的影响[J].华中农业大学学报,2004,23(5):581-586
[14]Chen W,Luo J K,Shen Q R.Effect of NH+4-N/NO-3-N ratios on growth and some physiological parameter of Chinese cabbage cultivars[J].Pedosphere,2005,15(3):310-318
[15]王丹英,汪自强.播期、密度、氮肥用量对菜用大豆产量和品质的效应[J].浙江大学学报:农业与生命科学版,2001,27(1):69-72
[16]贾彦博,杨肖娥,刘建祥.植物根系对养分缺乏和毒害的适应及其与养分吸收效率的关系[J].土壤通报,2005,36(4):610-616
[17]董守坤,刘丽君,孙聪姝,等.利用15 N标记研究氮素水平对大豆根瘤生长的影响[J].植物营养与肥料学报,2011,17(4):985-988
[18]宋海星,申斯乐,马淑英,等.硝态氮和氨态氮对大豆根瘤固氮的影响[J].大豆科学,1997,16(4):284-286
[2]刘晓静,刘艳楠.供氮水平对不同紫花苜蓿产量及品质的影响[J].草地学报,2013,7(4):702-707
[3]张彦东,范志强,王庆成,等.不同形态N素对水曲柳幼苗生长的影响[J].应用生态学报,2000,11(5):665-667
[4]司江英,王晓丽,陈冬梅,等.不同pH和氮素形态对作物幼苗生长的影响[J].扬州大学学报:农业与生命科学版,2007,28(3):68-71
[5]刘晓静,蒯佳林,李文卿,等.硝态氮与铵态氮对紫花苜蓿根系生长及结瘤固氮的影响[J].甘肃农业大学学报,2011,46(5):106-110
[6]俞艳,刘晓静,齐敏兴,等.pH和氮素形态对紫花苜蓿根瘤特性的影响[J].草原与草坪,2012,32(5):7-11
[7]Duxbury J M,Cherney J H.Inorganic nitrogen supply and symbiotic dinitro-gen fixation in alfalfa[J].Journal of Plant Nutrition,1994,17(12):2053-2067
[8]董守坤,刘丽君.利用15 N标记研究铵态氮与硝态氮对大豆的营养作用[J].大豆科学,2012,31(6):912-914
[9]王媛媛,柳小妮,柏东山.三个南方紫花苜蓿品种的耐盐性及其再生体系的建立[J].甘肃农业大学学报,2010,45(2):127-133
[10]孙建光,张燕春,徐晶,等.高效固氮芽孢杆菌筛选及其生物学特性[J].中国农业科学,2009,42(6):2043-2051
[11]蒯佳林,刘晓静,李文卿.不同氮素水平对接种根瘤菌紫花苜蓿生长特性的影响[J].草原与草坪,2010,31(3):56-59
[12]蒋名川,解淑贞.蔬菜施肥[M].北京:农业出版社,1985
[13]曹翠玲,李生秀.氮素形态对作物生理特性及生长的影响[J].华中农业大学学报,2004,23(5):581-586
[14]Chen W,Luo J K,Shen Q R.Effect of NH+4-N/NO-3-N ratios on growth and some physiological parameter of Chinese cabbage cultivars[J].Pedosphere,2005,15(3):310-318
[15]王丹英,汪自强.播期、密度、氮肥用量对菜用大豆产量和品质的效应[J].浙江大学学报:农业与生命科学版,2001,27(1):69-72
[16]贾彦博,杨肖娥,刘建祥.植物根系对养分缺乏和毒害的适应及其与养分吸收效率的关系[J].土壤通报,2005,36(4):610-616
[17]董守坤,刘丽君,孙聪姝,等.利用15 N标记研究氮素水平对大豆根瘤生长的影响[J].植物营养与肥料学报,2011,17(4):985-988
[18]宋海星,申斯乐,马淑英,等.硝态氮和氨态氮对大豆根瘤固氮的影响[J].大豆科学,1997,16(4):284-286