甲状腺癌组织中Skp2和Cx43的表达与患者临床病理特征的关系
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摘要
目的研究S期激酶相关蛋白2(Skp2)和细胞缝隙连接蛋白43(Cx43)在甲状腺癌组织中的表达,分析二者相关性。方法收集2012年3月至2015年8月42例甲状腺癌组织石蜡标本和20例甲状腺肿组织标本,采用免疫组织化学S-P法检测甲状腺癌组织中Skp2、Cx43的表达,数据均纳入SPSS20.0软件分析。Skp2、Cx43蛋白阳性表达率采用例(%)表示,行χ~2检验; Skp2与Cx43相关性分析采用Spearman检验。P<0.05差异有统计学意义。结果甲状腺肿组织Skp2蛋白阳性表达率25.0%明显低于甲状腺癌组织75.0%(χ~2=8.45,P<0.05);甲状腺肿组织Cx43蛋白阳性表达率95.0%明显高于甲状腺癌组织47.6%(χ~2=9.65,P<0.05);单因素分析显示,病理分期、淋巴结转移、周围组织侵犯是甲状腺癌组织中Skp2与Cx43蛋白阳性表达率的影响因素(P<0.05);多因素分析显示,Skp2、Cx43阳性表达与肿瘤分期Ⅲ、Ⅳ期、有淋巴结转移和周围组织侵犯等因素有明显相关性(P<0.05);经Spearman相关性分析显示:Skp2、Cx43蛋白在甲状腺癌中表达呈负相关(r=-0.709,P<0.05)。结论 Skp2和Cx43均可能参与甲状腺癌的增殖、浸润及转移。Skp2、Cx43的表达情况可能作为预测甲状腺癌的恶性程度和预后的指标。
Objective To investigate the expression of S kinase associated protein 2(Skp2) and connexin 43(Cx43) in thyroid carcinoma, and explore the correlation with their clinicopathological characteristics. Methods From March 2012 to August 2015, 42 paraffin of thyroid carcinoma, and 20 goiter tissues were collected. Immunohistochemical S-P method was used to detect the expression of Skp2, Cx43 in thyroid carcinoma. The data were analyzed by SPSS20.0 software. The positive expression rate of Skp2, Cx43 protein was expressed as n(%), and compared with χ~2 test. The correlation between Skp2 and Cx43 was analyzed by Spearman test. P<0.05 was statistically significant. Results The positive expression rate of Skp2 protein in goiter(25.0%) was significantly lower than that in thyroid carcinoma(75.0%), The difference was significant(χ~2=8.45, P<0.05); The positive expression rate of Cx43 protein in goiter(95.0%) was significantly higher than that in thyroid carcinoma(47.6%). The difference was significant(χ~2=9.65, P<0.05); Univariate analysis showed that pathological stage, lymph node metastasis and peripheral tissue invasion were the factors influencing the expression of Skp2 and Cx43 protein in thyroid carcinoma(P<0.05). Multivariate analysis showed that the positive expression of Skp2 and Cx43 was significantly correlated with the stage Ⅲ and Ⅳ of tumor, lymph node metastasis and peripheral tissue invasion(P<0.05).Spearman correlation analysis showed that: there was a negative correlation between Skp2 and Cx43 protein expression in thyroid carcinoma(r=-0.709, P<0.05). Conclusion Both Skp2 and Cx43 can participate in the proliferation,invasion and metastasis of thyroid carcinoma. The expression of Skp2 and Cx43 may be used as a predictor of malignant degree and prognosis of thyroid carcinoma.
Objective To investigate the expression of S kinase associated protein 2(Skp2) and connexin 43(Cx43) in thyroid carcinoma, and explore the correlation with their clinicopathological characteristics. Methods From March 2012 to August 2015, 42 paraffin of thyroid carcinoma, and 20 goiter tissues were collected. Immunohistochemical S-P method was used to detect the expression of Skp2, Cx43 in thyroid carcinoma. The data were analyzed by SPSS20.0 software. The positive expression rate of Skp2, Cx43 protein was expressed as n(%), and compared with χ~2 test. The correlation between Skp2 and Cx43 was analyzed by Spearman test. P<0.05 was statistically significant. Results The positive expression rate of Skp2 protein in goiter(25.0%) was significantly lower than that in thyroid carcinoma(75.0%), The difference was significant(χ~2=8.45, P<0.05); The positive expression rate of Cx43 protein in goiter(95.0%) was significantly higher than that in thyroid carcinoma(47.6%). The difference was significant(χ~2=9.65, P<0.05); Univariate analysis showed that pathological stage, lymph node metastasis and peripheral tissue invasion were the factors influencing the expression of Skp2 and Cx43 protein in thyroid carcinoma(P<0.05). Multivariate analysis showed that the positive expression of Skp2 and Cx43 was significantly correlated with the stage Ⅲ and Ⅳ of tumor, lymph node metastasis and peripheral tissue invasion(P<0.05).Spearman correlation analysis showed that: there was a negative correlation between Skp2 and Cx43 protein expression in thyroid carcinoma(r=-0.709, P<0.05). Conclusion Both Skp2 and Cx43 can participate in the proliferation,invasion and metastasis of thyroid carcinoma. The expression of Skp2 and Cx43 may be used as a predictor of malignant degree and prognosis of thyroid carcinoma.
引文
[1] 田文,赵文新.腔镜分化型甲状腺癌根治术的难点和争议[J/CD].中华普外科手术学杂志(电子版),2018,12(6):451-454.
[2] 杜峻峰,安然,李世拥.分化型甲状腺癌术中纳米碳示踪对颈淋巴结清扫的指导意义[J/CD].中华普外科手术学杂志(电子版),2018,12(6):462-465.
[3] 李贺,张凯华,杨家慧,等.甲状腺癌标志物的研究进展[J].转化医学电子杂志,2018,5(1):57-62.
[4] 中国抗癌协会甲状腺癌专业委员会(CATO).甲状腺癌血清标志物临床应用专家共识(2017版)[J].中国肿瘤临床,2018,45(1):7-13.
[5] 彭凯丽,陈宏辉.F-box蛋白家族及其与肿瘤相关问题的研究进展[J].实用医药杂志,2018,35(4):376-379.
[6] 周霖,张钰萍,何丽,等.乳腺癌组织中Cx43表达水平及其临床意义的Meta分析[J].中国现代医学杂志,2018,28(20):108-116.
[7] 梁佳,付杰军.Skp2在恶性肿瘤侵袭转移中的作用[J].现代肿瘤医学,2017,25(5):809-812.
[8] 陈玉恒,郭兰伟,张玥,等.中国2008年甲状腺癌发病、死亡和患病情况的估计及预测[J].中华疾病控制杂志,2014,18(3):200-203.
[9] 林岩松,李娇.2015年美国甲状腺学会《成人甲状腺结节与分化型甲状腺癌诊治指南》解读:分化型甲状腺癌～(131)I治疗新进展[J].中国癌症杂志,2016,26(1):1-12.
[10] 张春燕,杨倩.金丝桃苷预处理对心肌缺血再灌注性心律失常大鼠心肌ATP酶活性和Cx43、Kir2.1表达的影响[J].中成药,2018,40(2):254-260.
[11] 潘理会,霍峰,李春辉.Cx43、Shh与P-Ezrin蛋白在胃癌组织中的表达及意义[J].中国老年学杂志,2018,38(15):3736-3739.
[12] 周爱军,王洪勇,李振,等.甲状腺癌组织中BRMSI和Cx43的表达与患者临床病理及预后的关系[J].中国普通外科杂志,2017,26(2):259-264.
[13] 贾静,方健飞,任娟,等.宫颈癌HeLa细胞内SKP2结合蛋白的筛选和功能预测[J].中国肿瘤生物治疗杂志,2018,25(3):258-262.
[14] 李徳璇,李亚山,汪坚,等.非小细胞肺癌Survivin,Skp2和XIAP mRNA的表达及其临床意义[J].现代生物医学进展,2017,17(31):6159-6162,6200.
[15] 马秀梅,那日苏,王芳,等.甲状腺乳头状癌及相关病变Jab1、Skp2和p27蛋白的表达及意义[J].安徽医科大学学报,2017,52(1):124-129.
[16] 贺海燕,张淼,李晶,等.TRIM29、SKP2、MLH1在早期胃癌及癌前病变表达意义的临床研究[J].宁夏医学杂志,2017,39(8):775-777.
[17] 王海凤,陈天天,王月月,等.CXC趋化因子受体4通过S期激酶相关蛋白2调控乳腺癌细胞周期的机制[J].浙江大学学报(医学版),2017,46(4):357-363.
[2] 杜峻峰,安然,李世拥.分化型甲状腺癌术中纳米碳示踪对颈淋巴结清扫的指导意义[J/CD].中华普外科手术学杂志(电子版),2018,12(6):462-465.
[3] 李贺,张凯华,杨家慧,等.甲状腺癌标志物的研究进展[J].转化医学电子杂志,2018,5(1):57-62.
[4] 中国抗癌协会甲状腺癌专业委员会(CATO).甲状腺癌血清标志物临床应用专家共识(2017版)[J].中国肿瘤临床,2018,45(1):7-13.
[5] 彭凯丽,陈宏辉.F-box蛋白家族及其与肿瘤相关问题的研究进展[J].实用医药杂志,2018,35(4):376-379.
[6] 周霖,张钰萍,何丽,等.乳腺癌组织中Cx43表达水平及其临床意义的Meta分析[J].中国现代医学杂志,2018,28(20):108-116.
[7] 梁佳,付杰军.Skp2在恶性肿瘤侵袭转移中的作用[J].现代肿瘤医学,2017,25(5):809-812.
[8] 陈玉恒,郭兰伟,张玥,等.中国2008年甲状腺癌发病、死亡和患病情况的估计及预测[J].中华疾病控制杂志,2014,18(3):200-203.
[9] 林岩松,李娇.2015年美国甲状腺学会《成人甲状腺结节与分化型甲状腺癌诊治指南》解读:分化型甲状腺癌～(131)I治疗新进展[J].中国癌症杂志,2016,26(1):1-12.
[10] 张春燕,杨倩.金丝桃苷预处理对心肌缺血再灌注性心律失常大鼠心肌ATP酶活性和Cx43、Kir2.1表达的影响[J].中成药,2018,40(2):254-260.
[11] 潘理会,霍峰,李春辉.Cx43、Shh与P-Ezrin蛋白在胃癌组织中的表达及意义[J].中国老年学杂志,2018,38(15):3736-3739.
[12] 周爱军,王洪勇,李振,等.甲状腺癌组织中BRMSI和Cx43的表达与患者临床病理及预后的关系[J].中国普通外科杂志,2017,26(2):259-264.
[13] 贾静,方健飞,任娟,等.宫颈癌HeLa细胞内SKP2结合蛋白的筛选和功能预测[J].中国肿瘤生物治疗杂志,2018,25(3):258-262.
[14] 李徳璇,李亚山,汪坚,等.非小细胞肺癌Survivin,Skp2和XIAP mRNA的表达及其临床意义[J].现代生物医学进展,2017,17(31):6159-6162,6200.
[15] 马秀梅,那日苏,王芳,等.甲状腺乳头状癌及相关病变Jab1、Skp2和p27蛋白的表达及意义[J].安徽医科大学学报,2017,52(1):124-129.
[16] 贺海燕,张淼,李晶,等.TRIM29、SKP2、MLH1在早期胃癌及癌前病变表达意义的临床研究[J].宁夏医学杂志,2017,39(8):775-777.
[17] 王海凤,陈天天,王月月,等.CXC趋化因子受体4通过S期激酶相关蛋白2调控乳腺癌细胞周期的机制[J].浙江大学学报(医学版),2017,46(4):357-363.