摘要
建立了固相萃取-高效液相色谱对植物油中9种微量酚酸类化合物进行测定的方法。从提取及净化方式、流动相组成、流动相流速以及色谱柱温度等条件优化9种酚酸类化合物的检测方法。结果表明,样品经正己烷溶解,二醇基固相萃取柱(Diol-SPE)净化,甲醇-水为流动相,梯度洗脱,流速0.3 mL/min,检测波长为280 nm时,9种酚酸类化合物的检测结果最好,该检测方法的回收率在91.35%~103.21%之间,日内及日间精密度的RSD范围分别在0.3%~0.9%和0.6%~1.1%之间。该方法快速、准确,具有较好的重复性及稳定性,适合对植物油样品中9种酚酸类化合物的检测,结果为植物油的分类及真假判定提供了参考。
This study optimized the experimental conditions including extraction and cleanup methods, column temperature, and solvent composition, flow rate of mobile phase for nine trace phenolic acids detection by high-performance liquid chromatography(HPLC) after solid-phase extraction(SPE) cleanup. The results showed that the samples were dissolved in hexane. Diol group based solid-phase extraction column(Diol-SPE) purification, methanol and water as mobile phase, gradient elution, flow rate of 0.3 mL/min, the detection wavelength was 280 nm, detection of 9 kinds of phenolic compounds. The recovery of the detection method at the range of 91.35 %~103.21 %. The RSD of intra-day and inter-day precision was between 0.3%~0.9% and 0.6%~1.1%, respectively. The method was rapid, accurate, reproducible and stable. It is suitable for the determination of 9 phenolic acids in oil samples, and be able to provide a theoretical basis for the classification and judge determination of oils by analyzing and comparing the types and contents of phenolic acids in 16 batches of oil.
引文
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