Slug蛋白、E-钙黏蛋白在非小细胞肺癌组织中的表达及其与肺癌预后的关系
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摘要
目的:测定非小细胞肺癌组织中Slug蛋白、E-钙黏蛋白的表达水平,探讨二者与肺癌预后的关系。方法:采用免疫组化法检测68例非小细胞肺癌及30例癌旁组织中Slug蛋白、E-钙黏蛋白的表达情况。分析2种蛋白表达与临床病理特征及无进展生存期的关系。结果:非小细胞肺癌组织中Slug蛋白、E-钙黏蛋白的表达与癌旁组织中的表达相比差异有统计学意义(P<0.001)。Slug蛋白、E-钙黏蛋白的表达均与非小细胞肺癌的TNM分期、淋巴结转移有关(P<0.05)。非小细胞肺癌组织中Slug蛋白与E-钙黏蛋白的表达呈负相关(rS=-0.228,P=0.010)。Slug蛋白阳性表达的患者无进展生存期为11个月,阴性表达的患者为17个月,差异有统计学意义(P=0.036);E-钙黏蛋白阳性表达的患者无进展生存期为13个月,阴性表达的患者为11个月,差异具有统计学意义(P=0.003)。结论:Slug蛋白、E-钙黏蛋白在非小细胞肺癌的进展过程中起重要作用,两者可能存在相互作用,且与患者无进展生存期密切相关,可作为判断非小细胞肺癌预后的标志物。
Aim: To investigate the expressions of Slug and E-cadherin and the relationship with the prognosis in nonsmall-cell lung cancer( NSCLC). Methods: The expressions of Slug and E-cadherin in 68 specimens of NSCLC and 30 specimens of adjacent normal tissue were detected by immunohistochemistry. The relationship among the two kinds of protein expression and clinical pathological parameters and progression free survival( PFS) were analyzed. Results: There were significant difference in the expressions of the two proteins between the lung cancer tissue and adjacent normal tissue( P < 0. 001). The expressions of Slug and E-cadherin proteins were closely related with TNM stages and lymph node metastasis( P < 0. 05). The expression of Slug was negatively correlated with that of E-cadherin( rS=- 0. 228,P = 0. 010).The PFS of patients with positive expression of Slug protein was 11 months,while patients with negative expression was 17 months. and the difference was significant( P = 0. 036). The PFS of patients with positive expression of E-cadherin protein was 13 months,while patients with negative expression was 11 months,and the difference was significant( P = 0. 003).Conclusion: Slug and E-cadherin play important roles in the development of NSCLC,which are closely related with the PFS of NSCLC patients. They might be used as biomarkers in judging the prognosis of NSCLC.
Aim: To investigate the expressions of Slug and E-cadherin and the relationship with the prognosis in nonsmall-cell lung cancer( NSCLC). Methods: The expressions of Slug and E-cadherin in 68 specimens of NSCLC and 30 specimens of adjacent normal tissue were detected by immunohistochemistry. The relationship among the two kinds of protein expression and clinical pathological parameters and progression free survival( PFS) were analyzed. Results: There were significant difference in the expressions of the two proteins between the lung cancer tissue and adjacent normal tissue( P < 0. 001). The expressions of Slug and E-cadherin proteins were closely related with TNM stages and lymph node metastasis( P < 0. 05). The expression of Slug was negatively correlated with that of E-cadherin( rS=- 0. 228,P = 0. 010).The PFS of patients with positive expression of Slug protein was 11 months,while patients with negative expression was 17 months. and the difference was significant( P = 0. 036). The PFS of patients with positive expression of E-cadherin protein was 13 months,while patients with negative expression was 11 months,and the difference was significant( P = 0. 003).Conclusion: Slug and E-cadherin play important roles in the development of NSCLC,which are closely related with the PFS of NSCLC patients. They might be used as biomarkers in judging the prognosis of NSCLC.
引文
[1]SIEGEL R,NAISHADHAM D,JEMAL A.Cancer statistics,2013[J].CA Cancer J Clin,2013,63(1):11
[2]DAVID JM,RAJASEKARAN AK.Dishonorable discharge:the oncogenic roles of cleaved E-cadherin fragments[J].Cancer Res,2012,72(12):2917
[3]TOIYAMA Y,YASUDA H,SAIGUSA S,et al.Increased expression of Slug and Vimentin as novel predictive biomarkers for lymph node metastasis and poor prognosis in colorectal cancer[J].Carcinogenesis,2013,34(11):2548
[4]贺荣芳,胡忠良,沈明,等.胃癌组织中PTEN,VEGF,MMP-9的表达及相关性研究[J].中国普通外科杂志,2005,14(3):173
[5]许良中,杨文涛.免疫组织化学反应结果的判断标准[J].中国癌症杂志,1996,6(4):229
[6]NIETO MA.The ins and outs of the epithelial to mesenchymal transition in health and disease[J].Annu Rev Cell Dev Biol,2011,27:347
[7]张登勇,马翔,吴斌全,等.敲低MSX2对胰腺癌PANC-1细胞上皮-间充质转化的影响[J].南方医科大学学报,2015,32(2):179
[8]CASTRO ALVES C,ROSIVATZ E,SCHOTT C,et al.Slug is overexpressed in gastric carcinomas and may act synergistically with SIP1 and Snail in the down-regulation of Ecadherin[J].J Pathol,2007,211(5):507
[9]ROY F,BERX G.The cell-cell adhesion molecule E-cadherin[J].Cell Mol Life Sc,2008,65(23):3756
[10]RACHAGANI S,SENAPATI S,CHAKRABORTY S,et al.Activated Kras G12D is associated with invasion and metastasis of pancreatic cancer cells through inhibition of E-cadherin[J].Br J Cancer,2011,104(6):1038
[11]赵阳,许峰,任宏,等.E-cadherin基因启动子C-160A SNP与胃癌的相关性[J].西安交通大学学报(医学版),2013,2(2):214
[12]刘志娴,魏尔清,卢韵碧.上皮-间质转化在肿瘤发生发展中的作用研究进展[J].浙江大学学报(医学版),2015,44(2):211
[13]SANCHEZ-TILLE,LIU Y,DE BARRIOS O,et al.EMTactivating transcription factors in cancer:beyond EMT and tumor invasiveness[J].Cell Mol Life Sci,2012,69(20):3429
[14]FERNANDO RI,LITZINGER M,TRONO P,et al.The Tbox transcription factor Brachyury promotes epithelial-mesenchymal transition in human tumor cells[J].J Clin Invest,2010,120(2):533
[15]TANAKA Y,TERAI Y,KAWAGUCHI H,et al.Prognostic impact of EMT(epithelial-mesenchymal-transition)-related protein expression in endometrial cancer[J].Cancer Biol Ther,2013,14(1):13
[16]UCHIKADO Y,NATSUGOE S,OKUMURA H,et al.Slug expression in the E-cadherin preserved tumors is related to prognosis in patients with esophageal squamous cell carcinoma[J].Clin Cancer Res,2005,11(3):1174
[2]DAVID JM,RAJASEKARAN AK.Dishonorable discharge:the oncogenic roles of cleaved E-cadherin fragments[J].Cancer Res,2012,72(12):2917
[3]TOIYAMA Y,YASUDA H,SAIGUSA S,et al.Increased expression of Slug and Vimentin as novel predictive biomarkers for lymph node metastasis and poor prognosis in colorectal cancer[J].Carcinogenesis,2013,34(11):2548
[4]贺荣芳,胡忠良,沈明,等.胃癌组织中PTEN,VEGF,MMP-9的表达及相关性研究[J].中国普通外科杂志,2005,14(3):173
[5]许良中,杨文涛.免疫组织化学反应结果的判断标准[J].中国癌症杂志,1996,6(4):229
[6]NIETO MA.The ins and outs of the epithelial to mesenchymal transition in health and disease[J].Annu Rev Cell Dev Biol,2011,27:347
[7]张登勇,马翔,吴斌全,等.敲低MSX2对胰腺癌PANC-1细胞上皮-间充质转化的影响[J].南方医科大学学报,2015,32(2):179
[8]CASTRO ALVES C,ROSIVATZ E,SCHOTT C,et al.Slug is overexpressed in gastric carcinomas and may act synergistically with SIP1 and Snail in the down-regulation of Ecadherin[J].J Pathol,2007,211(5):507
[9]ROY F,BERX G.The cell-cell adhesion molecule E-cadherin[J].Cell Mol Life Sc,2008,65(23):3756
[10]RACHAGANI S,SENAPATI S,CHAKRABORTY S,et al.Activated Kras G12D is associated with invasion and metastasis of pancreatic cancer cells through inhibition of E-cadherin[J].Br J Cancer,2011,104(6):1038
[11]赵阳,许峰,任宏,等.E-cadherin基因启动子C-160A SNP与胃癌的相关性[J].西安交通大学学报(医学版),2013,2(2):214
[12]刘志娴,魏尔清,卢韵碧.上皮-间质转化在肿瘤发生发展中的作用研究进展[J].浙江大学学报(医学版),2015,44(2):211
[13]SANCHEZ-TILLE,LIU Y,DE BARRIOS O,et al.EMTactivating transcription factors in cancer:beyond EMT and tumor invasiveness[J].Cell Mol Life Sci,2012,69(20):3429
[14]FERNANDO RI,LITZINGER M,TRONO P,et al.The Tbox transcription factor Brachyury promotes epithelial-mesenchymal transition in human tumor cells[J].J Clin Invest,2010,120(2):533
[15]TANAKA Y,TERAI Y,KAWAGUCHI H,et al.Prognostic impact of EMT(epithelial-mesenchymal-transition)-related protein expression in endometrial cancer[J].Cancer Biol Ther,2013,14(1):13
[16]UCHIKADO Y,NATSUGOE S,OKUMURA H,et al.Slug expression in the E-cadherin preserved tumors is related to prognosis in patients with esophageal squamous cell carcinoma[J].Clin Cancer Res,2005,11(3):1174