养心颗粒对易损斑块Rho/Rho激酶信号通路血清LDL-C、TC、ET-1、TNF-α含量的影响
|
摘要
目的:观察养心颗粒对易损斑块Rho/Rho激酶信号通路血清LDL-C、TC、ET-1、TNF-α含量的影响。方法:随机将32只雄性家兔分为空白对照组、模型对照组、养心颗粒组、法舒地尔组。分别给予普通饲料及高脂饮食喂养8周,采用球囊损伤颈总动脉并注射P53基因转染建立易损斑块模型。药物治疗2周后采用ELISA法检测LDL-C、TC、ET-1、TNF-α水平。结果:与模型组比较,养心颗粒组和法舒地尔组血清中LDL-C、TC、ET-1、TNF-α含量下降(P <0. 05);养心颗粒组与法舒地尔组比较,血清中LDL-C,TC、ET-1、TNF-α含量,差异无统计学意义(P> 0. 05)。结论:养心颗粒可降低易损斑块Rho/Rho激酶信号通路血清中LDL-C、TC、ET-1、TNF-α的含量。
Objective: To observe the effect of Yangxin Granule on the levels of LDL-C,TC,ET-1,TNF-α in Rho/Rho kinase signaling pathway of vulnerable plaque. Methods: 32 healthy male rabbits were randomly divided into the blank group,the model group,Yangxin Granule group,and Fasudil group. The model of vulnerable plaque was established by eight-week high fat diet combined with the ballon-induced injury of common carotid artery and the injection of p53 gene. The levels of LDL-C,TC,ET-1,and TNF-α were measured 2 weeks after the treatment by ELISA method. Results: Compared to the model group,the serum levels of LDL-C,TC,ET-1,and TNF-α were decreased in Yangxin Granule group and Fasudil group( P < 0. 05); there was significant difference in serum levels of LDL-C between Yangxin Granule group and Fasudil group( P < 0. 05); no statistical difference was seen in terms of the serum levels of TC,ET-1,and TNF-α between these two groups( P > 0. 05). Conclusion: Yangxin Granule can reduce the serum levels of LDL-C,TC,ET-1 and TNF-α in Rho/Rho kinase signaling pathway of vulnerable plaque.
Objective: To observe the effect of Yangxin Granule on the levels of LDL-C,TC,ET-1,TNF-α in Rho/Rho kinase signaling pathway of vulnerable plaque. Methods: 32 healthy male rabbits were randomly divided into the blank group,the model group,Yangxin Granule group,and Fasudil group. The model of vulnerable plaque was established by eight-week high fat diet combined with the ballon-induced injury of common carotid artery and the injection of p53 gene. The levels of LDL-C,TC,ET-1,and TNF-α were measured 2 weeks after the treatment by ELISA method. Results: Compared to the model group,the serum levels of LDL-C,TC,ET-1,and TNF-α were decreased in Yangxin Granule group and Fasudil group( P < 0. 05); there was significant difference in serum levels of LDL-C between Yangxin Granule group and Fasudil group( P < 0. 05); no statistical difference was seen in terms of the serum levels of TC,ET-1,and TNF-α between these two groups( P > 0. 05). Conclusion: Yangxin Granule can reduce the serum levels of LDL-C,TC,ET-1 and TNF-α in Rho/Rho kinase signaling pathway of vulnerable plaque.
引文
[1]黄爱良,黄荣志,黄小倩,等.动脉粥样硬化动物模型构建的方法与现状[J].中国组织工程研究,2015,19(27):4423-4428.
[2]陶蕾,纪立伟,王艳春.他汀类药物对动脉粥样硬化的疗效研究[J].中国现代医学杂志,2017,27(14):93-98.
[3]田新蕊,刘国安.通冠丸对动脉粥样硬化大鼠血清CRP及IL-6含量的影响[J].西部中医药,2011,24(5):42-44.
[4]张光伟,陈玉龙.动脉粥样硬化形成的危险因素研究进展[J].现代医药卫生,2014,30(17):2603-2606.
[5]顾丽娟,张勤,李月华,等.养血清脑颗粒联合立普妥对动脉粥样硬化的影响[J].中风与神经疾病杂志,2018,35(1):56-58.
[6] Lin Y,Liu P,Adhikari N,et al. RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages[J]. J Mol Cell Cardiol,2014(12):287-294.
[7]吴泽民,刘智,刘畅,等.养心汤对心肌缺血血瘀证大鼠血脂的影响[J].长春中医药大学学报,2014,30(6):983-984.
[8]潘祥宾,刘影哲,周亚滨,等.养心颗粒对易损斑块家兔模型胆固醇7-α-羟化酶和MMP-9含量的影响[J].中医药信息,2012,29(4):60-62.
[9]陈娜,李芳. Rho/Rho激酶信号通路异常激活与心血管疾病[J].大连医科大学学报,2012,34(6):613-616.
[10]宋鑫.四氯化碳诱导大鼠肝纤维化不同阶段ROCK1表达的实验性研究[D].石家庄:河北医科大学,2010.
[11]马东蔚. Rho/ROCK信号通路对高糖诱导下人肾小球系膜细胞炎症反应及纤维化的影响[D].沈阳:中国医科大学,2011.
[12]国伟,孟建中. Rho/Rho激酶信号通路与血管内皮通透性的研究[J].生物医学工程研究,2009,28(2):154-158.
[13]傅威.机械牵张对肺血管内皮炎症反应、通透性的影响及作用机制研究[D].广州:广州医学院,2013.
[14]张效林,韩雅玲,康建. Rho GTP酶在内皮细胞通透性增高中的作用[J].国际呼吸杂志,2003,23(4):201-203.
[15]王盼,李言,张永. Rho GTP酶调节肺血管内皮屏障的研究进展[J].蚌埠医学院学报,2015,40(5):698-701.
[16]黄丽萍,杜晓刚. Rho GTP酶与血管内皮通透性的研究进展[J].中华医学教育探索杂志,2010,9(10):1439-1440.
[17] Zhou Q B,Wu L Q,Zhang Y,et al. Effects of Zhizi Chuanxiong Capsule(栀子川芎胶囊)on the Abnormal Methylation in Rabbits with Atherosclerosis[J]. Chinese Journal of Integrative Medicine,2018,24(7):1-6.
[18]许金鹏,刘洋,李靖,等. H型高血压合并颈动脉粥样硬化患者血清Hcy含量与炎症因子、斑块稳定性的相关性[J].中国现代医学杂志,2017,27(28):61-65.
[19]刘俊田.动脉粥样硬化发病的炎症机制的研究进展[J].西安交通大学学报(医学版),2015,36(2):141-152.
[20] Shu Wang,Bradley Miller,Nirupa R. Matthan,et al. Aortic cholesterol accumulation correlates with systemic inflammation but not hepatic and gonadal adipose tissue inflammation in low-density lipoprotein receptor null mice[J]. Nutrition Research,2013,33(12):32-35.
[21] Koh K K,Han S H,Oh P C,et al. Combination therapy for treatment or prevention of atherosclerosis:focus on the lipid-RAAS interaction[J].Atherosclerosis,2010,209(2):307.
[22]芦凤琴.早期动脉粥样硬化血管内皮细胞损伤机制研究进展[J].中国乡村医药,2011,18(7):76-77.
[23]车海蓝.葡萄籽提取物原花青素对动脉粥样硬化大鼠ABCA1、TNF—α、MCP—1表达的影响[D].新乡:新乡医学院,2014.
[24]习丹. Kv1. 3钾通道特异性阻断剂对动脉粥样硬化大鼠CD4+T淋巴细胞的影响和意义[D].厦门:厦门大学,2011.
[25]吴同启.宁心痛颗粒干预模型兔动脉粥样硬化易损斑块的作用及机制研究[D].南京:南京中医药大学,2011.
[26]陶蕾,纪立伟,王艳春.他汀类药物对动脉粥样硬化的疗效研究[J].中国现代医学杂志,2017,27(14):93-98.
[27]曹源,舒晓春,吴贻全,等.复方丹参对冠心病基质金属蛋白酶-9和肿瘤坏死因子-α的影响[J].心血管康复医学杂志,2005,14(4):319-321.
[28]李金阳,姚剑挺,盛思琪,等.细胞凋亡和有效吞噬在动脉粥样硬化斑块中的作用[J].基础医学与临床,2018,38(4):548-552.
[29]李银平,沈洪,黎檀实,等.全身炎症反应综合征患者血浆血管活性因子变化及临床意义[J].中国急救医学,2000,20(7):398-399.
[30] Zamor a M A,Dempsey EC,Walchak SJ,et al. BQ123,an ETA receptorantagonist,inhibits endothelin 1 mediated proliferation of human pulmonary arterysmooth muscle cells[J]. Am J Respir Cell Mol Biol,1993,9(4):329-433.
[31]叶逻湾,陈志强,叶志明.瑞舒伐他汀对高血脂症合并颈动脉粥样硬化患者的应用效果研究[J].临床医学,2018,38(4); 110-112.
[2]陶蕾,纪立伟,王艳春.他汀类药物对动脉粥样硬化的疗效研究[J].中国现代医学杂志,2017,27(14):93-98.
[3]田新蕊,刘国安.通冠丸对动脉粥样硬化大鼠血清CRP及IL-6含量的影响[J].西部中医药,2011,24(5):42-44.
[4]张光伟,陈玉龙.动脉粥样硬化形成的危险因素研究进展[J].现代医药卫生,2014,30(17):2603-2606.
[5]顾丽娟,张勤,李月华,等.养血清脑颗粒联合立普妥对动脉粥样硬化的影响[J].中风与神经疾病杂志,2018,35(1):56-58.
[6] Lin Y,Liu P,Adhikari N,et al. RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages[J]. J Mol Cell Cardiol,2014(12):287-294.
[7]吴泽民,刘智,刘畅,等.养心汤对心肌缺血血瘀证大鼠血脂的影响[J].长春中医药大学学报,2014,30(6):983-984.
[8]潘祥宾,刘影哲,周亚滨,等.养心颗粒对易损斑块家兔模型胆固醇7-α-羟化酶和MMP-9含量的影响[J].中医药信息,2012,29(4):60-62.
[9]陈娜,李芳. Rho/Rho激酶信号通路异常激活与心血管疾病[J].大连医科大学学报,2012,34(6):613-616.
[10]宋鑫.四氯化碳诱导大鼠肝纤维化不同阶段ROCK1表达的实验性研究[D].石家庄:河北医科大学,2010.
[11]马东蔚. Rho/ROCK信号通路对高糖诱导下人肾小球系膜细胞炎症反应及纤维化的影响[D].沈阳:中国医科大学,2011.
[12]国伟,孟建中. Rho/Rho激酶信号通路与血管内皮通透性的研究[J].生物医学工程研究,2009,28(2):154-158.
[13]傅威.机械牵张对肺血管内皮炎症反应、通透性的影响及作用机制研究[D].广州:广州医学院,2013.
[14]张效林,韩雅玲,康建. Rho GTP酶在内皮细胞通透性增高中的作用[J].国际呼吸杂志,2003,23(4):201-203.
[15]王盼,李言,张永. Rho GTP酶调节肺血管内皮屏障的研究进展[J].蚌埠医学院学报,2015,40(5):698-701.
[16]黄丽萍,杜晓刚. Rho GTP酶与血管内皮通透性的研究进展[J].中华医学教育探索杂志,2010,9(10):1439-1440.
[17] Zhou Q B,Wu L Q,Zhang Y,et al. Effects of Zhizi Chuanxiong Capsule(栀子川芎胶囊)on the Abnormal Methylation in Rabbits with Atherosclerosis[J]. Chinese Journal of Integrative Medicine,2018,24(7):1-6.
[18]许金鹏,刘洋,李靖,等. H型高血压合并颈动脉粥样硬化患者血清Hcy含量与炎症因子、斑块稳定性的相关性[J].中国现代医学杂志,2017,27(28):61-65.
[19]刘俊田.动脉粥样硬化发病的炎症机制的研究进展[J].西安交通大学学报(医学版),2015,36(2):141-152.
[20] Shu Wang,Bradley Miller,Nirupa R. Matthan,et al. Aortic cholesterol accumulation correlates with systemic inflammation but not hepatic and gonadal adipose tissue inflammation in low-density lipoprotein receptor null mice[J]. Nutrition Research,2013,33(12):32-35.
[21] Koh K K,Han S H,Oh P C,et al. Combination therapy for treatment or prevention of atherosclerosis:focus on the lipid-RAAS interaction[J].Atherosclerosis,2010,209(2):307.
[22]芦凤琴.早期动脉粥样硬化血管内皮细胞损伤机制研究进展[J].中国乡村医药,2011,18(7):76-77.
[23]车海蓝.葡萄籽提取物原花青素对动脉粥样硬化大鼠ABCA1、TNF—α、MCP—1表达的影响[D].新乡:新乡医学院,2014.
[24]习丹. Kv1. 3钾通道特异性阻断剂对动脉粥样硬化大鼠CD4+T淋巴细胞的影响和意义[D].厦门:厦门大学,2011.
[25]吴同启.宁心痛颗粒干预模型兔动脉粥样硬化易损斑块的作用及机制研究[D].南京:南京中医药大学,2011.
[26]陶蕾,纪立伟,王艳春.他汀类药物对动脉粥样硬化的疗效研究[J].中国现代医学杂志,2017,27(14):93-98.
[27]曹源,舒晓春,吴贻全,等.复方丹参对冠心病基质金属蛋白酶-9和肿瘤坏死因子-α的影响[J].心血管康复医学杂志,2005,14(4):319-321.
[28]李金阳,姚剑挺,盛思琪,等.细胞凋亡和有效吞噬在动脉粥样硬化斑块中的作用[J].基础医学与临床,2018,38(4):548-552.
[29]李银平,沈洪,黎檀实,等.全身炎症反应综合征患者血浆血管活性因子变化及临床意义[J].中国急救医学,2000,20(7):398-399.
[30] Zamor a M A,Dempsey EC,Walchak SJ,et al. BQ123,an ETA receptorantagonist,inhibits endothelin 1 mediated proliferation of human pulmonary arterysmooth muscle cells[J]. Am J Respir Cell Mol Biol,1993,9(4):329-433.
[31]叶逻湾,陈志强,叶志明.瑞舒伐他汀对高血脂症合并颈动脉粥样硬化患者的应用效果研究[J].临床医学,2018,38(4); 110-112.