杜鹃兰种子萌发适宜培养基的筛选
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摘要
以人工授粉获得的杜鹃兰种子为试材,研究了不同基本培养基、不同有机添加物、蔗糖浓度和不同植物生长调节剂对杜鹃兰种子萌发的影响,筛选适于杜鹃兰种子萌发的培养基。结果表明:KC基本培养基上种子萌发较好,萌发率达17.38%;添加7.5%土豆泥对种子萌发较好,萌发率为15.36%;蔗糖浓度为1.0%时,种子有较高萌发率,为14.20%;添加0.5mg·L~(-1)KT或1.5mg·L~(-1) IBA时适于种子萌发,萌发率分别为36.19%、30.00%;正交实验得出适于杜鹃兰种子萌发的培养基为KC+0.5mg·L~(-1) KT(激动素)+1.3mg·L~(-1) IBA(吲哚乙酸)+1.0%蔗糖+7.5%土豆泥+0.5%活性炭,萌发率达32.87%,萌发时间约6周。
Cremastra appendiculata seeds from artificial pollination were used as test materials to screen the suitable medium for seeds germination.The test contents involved the effects of the basic medium,organic additives,sucrose concentration,and growth regulators to seeds germination of C.appendiculata.The results showed that seeds which was in KC basic medium had higher germination rate(17.38%).Adding 7.5% mashed potatoes into KC medium was beneficial to seeds germination,and the germination rate was 15.36% The seeds germination rate was higher(14.20%)when sucrose concentration was 1.0%.0.5mg·L~(-1) KT or 1.5mg·L~(-1)IBA was the suitable growth regulators seeds germination and the germinationrate was 36.19% and 30.00%,respectively.In conclusion,the best medium for seeds germination of C.appendiculata was KC+0.5mg·L~(-1)KT+1.3mg·L~(-1) IBA+1.0% sucrose+7.5% mashed potatoes+0.5% AC,which seeds germination rate reached 32.87%and germination time was 6 weeks.
Cremastra appendiculata seeds from artificial pollination were used as test materials to screen the suitable medium for seeds germination.The test contents involved the effects of the basic medium,organic additives,sucrose concentration,and growth regulators to seeds germination of C.appendiculata.The results showed that seeds which was in KC basic medium had higher germination rate(17.38%).Adding 7.5% mashed potatoes into KC medium was beneficial to seeds germination,and the germination rate was 15.36% The seeds germination rate was higher(14.20%)when sucrose concentration was 1.0%.0.5mg·L~(-1) KT or 1.5mg·L~(-1)IBA was the suitable growth regulators seeds germination and the germinationrate was 36.19% and 30.00%,respectively.In conclusion,the best medium for seeds germination of C.appendiculata was KC+0.5mg·L~(-1)KT+1.3mg·L~(-1) IBA+1.0% sucrose+7.5% mashed potatoes+0.5% AC,which seeds germination rate reached 32.87%and germination time was 6 weeks.
引文
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[34]JOHNSON T R,STEWART S L,DUTRA D,et al.Asymbiotic and symbiotic seed germination of Eulophia alta(Orchidaceae):preliminary evidence for the symbioticculture advantage[J].Plant Cell,Tissue and Organ Culture,2007,90:313-323.
[35]ARDITTI J.Factors affecting the germination of orchid seeds[J].Botanical Review,1967,33:1-97.
[2]ZHANG M S,WU S J,JIE X J,et al.Effect of endophyte extract on micropropagation of Cremastra appendiculata(D.Don.)Makino(Orchidaceae)[J].Propagation of Ornamental Plants,2006,6(2):83-89.
[3]田昌海,王世清.山慈菇的研究进展[J].现代医药卫生,2008,24(7):1009-1010.
[4]朱国胜.贵州特色药用兰科植物杜鹃兰和独蒜兰共生真菌研究与应用[D].武汉:华中农业大学,2009.
[5]郭顺星,徐锦堂.兰科植物种子无菌萌发的研究[J].种子,1990,49(5):36-37,58.
[6]张明生,戚金亮,刘志,等.药用兰科植物杜鹃兰的组织培养与快速繁殖[J].种子,2005,24(8):82.
[7]SHIM J S,KIM J H,LEE J,et al.Anti-angiogenic activity of a homoisoflavanone from Cremastra appendiculata[J].Plant Medica,2004,70(2):171-173.
[8]薛震,李帅,王素娟,等.山慈菇Cremastra appendiculata化学成分[J].中国中药杂志,2005,30(7):511-513.
[9]夏文斌,薛震,李帅,等.杜鹃兰化学成分及肿瘤细胞毒活性研究[J].中国中药杂志,2005,30(23):1828-1830.
[10]阮小丽,施大文.山慈菇的抗肿瘤及抑菌作用[J].中草药,2009,32(12):1886-1888.
[11]毛堂芬,刘涛,刘作易,等.杜鹃兰离体快繁技术研究[J].中药材,2007,30(9):1057-1059.
[12]丁兰,王丽,李淮,等.卡德丽亚兰种子非共生萌发及萌发过程中原球茎发育的细胞学研究[J].广西植物,2007,27(6):909-912.
[13]王钟,马永清,贾锦楠,等.马铃薯对瓜列当种子萌发的化感作用研究[J].中国生态农业学报,2013,21(3):333-339.
[14]尤超,宋晓婕,石彩娟,等.不同培养基对山慈菇种子无菌萌发的影响[J].安徽农业科学,2011,39(26):16006-16007,16012.
[15]王莲辉,姜运力,余金勇,等.西藏虎头兰的组织培养与快速繁殖[J].植物生理学通讯,2009,45(1):51-52.
[16]方中明,吴坤林,陈之林,等.湿唇兰的无菌播种和快速繁殖[J].植物生理学通讯,2008,44(3):529-530.
[17]文慧婷,张翠玲,吴刚.香草兰的组织培养试验[J].广东农业科学,2008(11):35-36.
[18]余乐,兰芹英,王晓静,等.3种石斛兰种子非共生萌发和离体保存[J].安徽农学通报,2009,15(8):27-28,69.
[19]罗远华,冷青云,莫饶,等.冬凤兰非共生萌发和低温离体保存[J].安徽农业科学,2008,36(19):8068-8069,8119.
[20]王建勤,陈钢,林兰英.金线莲原球茎的组培诱导[J].中药材,1995,18(1):3-5.
[21]卢思聪,薛秀玲.建兰与多花兰杂交胚培养中植物激素的应用[J].种子,1982,4(2):27-29.
[22]段金玉.植物激素和培养基对天麻种子萌发的影响[J].云南植物研究,1984,6(2):234.
[23]杨美纯,周歧伟,许鸿源.蝴蝶兰的种子培养[J].广西农业生物科学,2002,21(4):258-260.
[24]丁兰,张丽,郭柳,等.濒危植物佛手参种子的非共生萌发及种苗的快速繁殖[J].植物生理学报,2014,50(1):77-82.
[25]于敏,徐恒,张华,等.植物激素在种子休眠与萌发中的调控机制[J].植物生理学报,2016,52(5):599-606.
[26]黄家林,胡虹.黄花杓兰种子无菌萌发的培养条件研究[J].植物分类与资源学报,2001,23(1):105-108.
[27]黄磊,贺莜蓉,郑立明,等.春兰种子非共生萌发的研究[J].种子,2003(6):40-41.
[28]邓莲,张毓,王苗苗,等.濒危兰科植物大花杓兰种子非共生萌发的研究[J].种子,2012,31(6):31-34,39.
[29]徐晓薇,林绍生,姚丽娟,等.蝴蝶兰种胚萌发影响因素的研究[J].浙江农业学报,2004,16(4):28-31.
[30]伍成厚,叶秀粦,梁承邺.五唇兰种子离体培养的研究[J].广西植物,2005,25(2):149-151.
[31]方香香,谢欢,伍奥林,等.霍山石斛快速繁殖技术研究[J].中国现代中药,2015(6):529-532.
[32]丘亮伟,王建,肖丽红,等.蝴蝶兰无菌播种及快繁技术研究[J].广西农业科学,2009(12):1523-1525.
[33]宋智琴,杨平飞,罗鸣,等.不同添加物对白及组培壮苗培养的影响[J].贵州农业科学,2016,44(3):138-140.
[34]JOHNSON T R,STEWART S L,DUTRA D,et al.Asymbiotic and symbiotic seed germination of Eulophia alta(Orchidaceae):preliminary evidence for the symbioticculture advantage[J].Plant Cell,Tissue and Organ Culture,2007,90:313-323.
[35]ARDITTI J.Factors affecting the germination of orchid seeds[J].Botanical Review,1967,33:1-97.