维生素E辛烯基琥珀酸酯的定性分析
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摘要
通过比较不同高效液相色谱(HPLC)方法对维生素E与辛烯基琥珀酸酐反应产物分离效果的影响,最终建立了以Dionex Kromasil C18柱为分析柱、甲醇和0.033 mol/L H3PO4(100:1,体积比)为流动相的色谱分离条件;借助高效液相色谱-大气压化学电离源质谱联用(HPLC-APCI-MS)法检测分离得到的色谱峰,通过比较它们的紫外光谱和质谱特征,确定其中两种物质为维生素E辛烯基琥珀酸酯的异构体。
The effect of high performance liquid chromatographic(HPLC) method on the separation of the reaction product of vitamin E and octenylsuccinic anhydride was compared,and then a HPLC system consisting of a Dionex Kromasil C18 column and the mobile phase of methanol and 0.033 mol/L phosphoric acid(100/1,V/V) was established.Using the combination of UV spectra and HPLC-atmospheric pressure chemical ionization(APCI)-mass spectrometric(MS) data,two substances were identified as the isomers of vitamin E octenylsuccinate.
The effect of high performance liquid chromatographic(HPLC) method on the separation of the reaction product of vitamin E and octenylsuccinic anhydride was compared,and then a HPLC system consisting of a Dionex Kromasil C18 column and the mobile phase of methanol and 0.033 mol/L phosphoric acid(100/1,V/V) was established.Using the combination of UV spectra and HPLC-atmospheric pressure chemical ionization(APCI)-mass spectrometric(MS) data,two substances were identified as the isomers of vitamin E octenylsuccinate.
引文
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