高效液相色谱法测定除虫脲原药中对氯苯胺的含量
|
摘要
[目的]建立一种高效液相色谱测定除虫脲原药中对氯苯胺含量的分析方法。[方法]采用高效液相色谱法,使用Kromosil-C_(18)色谱柱,以乙腈-水为流动相进行梯度洗脱,流速为1 m L/min,在242 nm波长下对除虫脲原药中对氯苯胺进行定量分析。[结果]当对氯苯胺质量浓度在0.06~2.50 mg/L范围内,方法的线性相关系数为0.9997,平均回收率为110.9%。[结论]方法准确、快速,适用于除虫脲原药及其他产品中对氯苯胺残留的定量分析。
[Aims] A HPLC method was developed for the quantitative determination of p-chloroaniline content in diflubenzuron TC. [Methods] The content of p-chloroaniline was quantitatively determined by HPLC with the Kromosil-C_(18) column as fix phase and acetonitrile-water as mobile phase. The wavelength was 242 nm, and the flow rate was 1 m L/min. [Results]The results showed that when the concentration of p-chloroaniline ranged from 0.06 to2.50 mg/L, the linear correlation coefficient was 0.9997, and the average recovery was 110.9%. [Conclusions]The method is simple and accurate, which is suitable for the quantitative analysis of p-chloroaniline in diflubenzuron TC or other products.
[Aims] A HPLC method was developed for the quantitative determination of p-chloroaniline content in diflubenzuron TC. [Methods] The content of p-chloroaniline was quantitatively determined by HPLC with the Kromosil-C_(18) column as fix phase and acetonitrile-water as mobile phase. The wavelength was 242 nm, and the flow rate was 1 m L/min. [Results]The results showed that when the concentration of p-chloroaniline ranged from 0.06 to2.50 mg/L, the linear correlation coefficient was 0.9997, and the average recovery was 110.9%. [Conclusions]The method is simple and accurate, which is suitable for the quantitative analysis of p-chloroaniline in diflubenzuron TC or other products.
引文
[1]HELIA R,ROBINSON G K,BULL A T.Characterization of a Soil-derived Bacterial Consortium Degrading 4-Chloroaniline[J].Microbiology,2003,149:3279-3287.
[2]COMMISSION DIRECTIVE 2008/69/EC of 1 July 2008[Z].Official Journal of the European Union,2.7.2008:L172/13.
[3]周春兰,肖艳红.气相色谱法测定对氨基苯乙醚中杂质对氯苯胺的含量[J].黑龙江医药,2003,16(5):439-440.
[4]李秀瑜,侯都兴,赵爱东.反相高效液相色谱法测定对硝基氯苯、对氯苯胺和苯胺[J].精细石油化工,2008,25(4):52-54.
[5]钱微君,金美菊,秦军英.气相色谱-质谱法测定纺织品中对氯苯胺[J].理化检验-化学分册,2011,47(5):566-568.
[6]海勇,田树盛.气相色谱/质谱法检测棉布样品还原后的联苯胺[J].北京服装学院学报:自然科学版,2004,24(4):56-60.
[7]郑娟,苏红伟,陈如.现代分析测试技术在印染行业的应用(五)—气质联用技术及其在纺织工业上的应用[J].印染,2007(7):40-43.
[8]CIPAC Method 339/TK/M/2[M].Handbook H,1998:141.
[2]COMMISSION DIRECTIVE 2008/69/EC of 1 July 2008[Z].Official Journal of the European Union,2.7.2008:L172/13.
[3]周春兰,肖艳红.气相色谱法测定对氨基苯乙醚中杂质对氯苯胺的含量[J].黑龙江医药,2003,16(5):439-440.
[4]李秀瑜,侯都兴,赵爱东.反相高效液相色谱法测定对硝基氯苯、对氯苯胺和苯胺[J].精细石油化工,2008,25(4):52-54.
[5]钱微君,金美菊,秦军英.气相色谱-质谱法测定纺织品中对氯苯胺[J].理化检验-化学分册,2011,47(5):566-568.
[6]海勇,田树盛.气相色谱/质谱法检测棉布样品还原后的联苯胺[J].北京服装学院学报:自然科学版,2004,24(4):56-60.
[7]郑娟,苏红伟,陈如.现代分析测试技术在印染行业的应用(五)—气质联用技术及其在纺织工业上的应用[J].印染,2007(7):40-43.
[8]CIPAC Method 339/TK/M/2[M].Handbook H,1998:141.