亚甲蓝分光光度法测定水中阴离子表面活性剂含量
|
摘要
基于阴离子表面活性剂能与亚甲蓝阳离子反应生成蓝色物质,该物质被三氯甲烷萃取分离,可在波长652nm处,采用分光光度法测定水中阴离子表面活性剂的含量。优化的试验条件如下:1 0.09g·L~(-1)碱性亚甲蓝溶液的用量为25mL;2萃取条件:萃取3次,每次45s。用亚甲蓝溶液的碱性去除蛋白质和季铵类化合物的负干扰。阴离子表面活性剂的质量浓度在0.100~2.00mg·L~(-1)范围内与其吸光度呈线性关系,检出限(3s)为0.005 mg·L~(-1)。加标回收率在91.0%~99.3%之间,测定值的相对标准偏差(n=6)均小于5.0%。
Based on the electrostatic reaction between anionic surfactants and the cation of methylene blue to form a blue compound which can be extracted with CHCl_3 and to have its absorbance detected at the wavelength of652 nm.Spectrophotometry was applied to the determination of anionic surfactants in water.The optimized conditions found were as follows:1 amount of 0.09g·L~(-1) alkaline methylene blue solution added:25 mL;2condition of extraction:extract for 3times with 45 sin each extraction.Interferences of protein and quaternary ammonium compounds were eliminated by the alkalinity of the methylene blue solution.Linear relationship between values of absorbance and mass concentration of anionic surfactants was obtained in the range of 0.100-2.00mg·L~(-1),with detection limit(3s)of 0.005mg·L~(-1).Values of recovery found by standard addition method were in the range of 91.0%-99.3%,and values of RSD′s(n=6)less than 5.0%.
Based on the electrostatic reaction between anionic surfactants and the cation of methylene blue to form a blue compound which can be extracted with CHCl_3 and to have its absorbance detected at the wavelength of652 nm.Spectrophotometry was applied to the determination of anionic surfactants in water.The optimized conditions found were as follows:1 amount of 0.09g·L~(-1) alkaline methylene blue solution added:25 mL;2condition of extraction:extract for 3times with 45 sin each extraction.Interferences of protein and quaternary ammonium compounds were eliminated by the alkalinity of the methylene blue solution.Linear relationship between values of absorbance and mass concentration of anionic surfactants was obtained in the range of 0.100-2.00mg·L~(-1),with detection limit(3s)of 0.005mg·L~(-1).Values of recovery found by standard addition method were in the range of 91.0%-99.3%,and values of RSD′s(n=6)less than 5.0%.
引文
[1]杜娟.同步荧光分析法测定阴离子表面活性剂[J].日用化学工业,2006,36(5):321-323.
[2]莫苹,黄玉明.HPLC-UV检测法测定废水中十二烷基苯磺酸钠[J].西南大学学报,2007,29(1):65-68.
[3]袁晶,王小红,谢家理.罗丹明B共振光散射法测定阴离子表面活性剂[J].理化检验-化学分册,2006,42(1):24-26.
[4]魏良,张新申,代以春,等.流动注射分光光度法测定环境水中阴离子表面活性剂[J].理化检验-化学分册,2009,45(9):1077-1079.
[5]GB 7494-1987水质阴离子表面活性剂的测定亚甲蓝分光光度法[S].
[6]李光.水中阴离子表面活性剂分析方法研究进展[J].环境科技,2010(1):96-99.
[7]娄明华,施新锋.流动注射法测定水中阴离子表面活性剂的探讨[J].四川环境,2012(1):46-48.
[8]郭少维,宋东升,陈星.流动注射分光光度法测定水中阴离子表面活性剂[J].科技信息(学术研究),2008(34):3-4.
[9]李文举,徐海丽.流动注射分光光度法测定水质中阴离子表面活性剂[J].环保科技,2013,19(2):17-19.
[10]季鹏,范美娟,程芳菲.流动注射法测量水中洗涤剂的方法改进[J].广东化工,2014(7):3-5.
[11]韩清,胡迅,徐立,等.LACHATQC8500流动注射测定地表水中阴离子合成洗涤剂[J].中国卫生检疫,2012,22(8):1800-1802.
[12]叶蔚霞.SKALAR San++型连续流动注射分析仪测定水中阴离子表面活性剂的应用探讨[J].化学工程与装备,2014(6):178-180.
[13]王婉.Seal-AA3流动注射测定饮用水中阴离子合成洗涤剂[J].中国卫生检疫,2014,24(14):1994-1996.
[2]莫苹,黄玉明.HPLC-UV检测法测定废水中十二烷基苯磺酸钠[J].西南大学学报,2007,29(1):65-68.
[3]袁晶,王小红,谢家理.罗丹明B共振光散射法测定阴离子表面活性剂[J].理化检验-化学分册,2006,42(1):24-26.
[4]魏良,张新申,代以春,等.流动注射分光光度法测定环境水中阴离子表面活性剂[J].理化检验-化学分册,2009,45(9):1077-1079.
[5]GB 7494-1987水质阴离子表面活性剂的测定亚甲蓝分光光度法[S].
[6]李光.水中阴离子表面活性剂分析方法研究进展[J].环境科技,2010(1):96-99.
[7]娄明华,施新锋.流动注射法测定水中阴离子表面活性剂的探讨[J].四川环境,2012(1):46-48.
[8]郭少维,宋东升,陈星.流动注射分光光度法测定水中阴离子表面活性剂[J].科技信息(学术研究),2008(34):3-4.
[9]李文举,徐海丽.流动注射分光光度法测定水质中阴离子表面活性剂[J].环保科技,2013,19(2):17-19.
[10]季鹏,范美娟,程芳菲.流动注射法测量水中洗涤剂的方法改进[J].广东化工,2014(7):3-5.
[11]韩清,胡迅,徐立,等.LACHATQC8500流动注射测定地表水中阴离子合成洗涤剂[J].中国卫生检疫,2012,22(8):1800-1802.
[12]叶蔚霞.SKALAR San++型连续流动注射分析仪测定水中阴离子表面活性剂的应用探讨[J].化学工程与装备,2014(6):178-180.
[13]王婉.Seal-AA3流动注射测定饮用水中阴离子合成洗涤剂[J].中国卫生检疫,2014,24(14):1994-1996.