贵州省2017年蚊虫及蚊传虫媒病毒调查研究
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摘要
目的了解贵州省东北部地区蚊虫以及蚊传虫媒病毒的种类及其分布。方法 2017年在贵州省德江、江口县村庄的动物饲养圈使用诱蚊灯采集蚊虫,现场分类并冻存于液氮备用。采集的蚊虫标本在实验室处理后接种BHK-21和C6/36细胞分离病毒,同时进行标本中病毒基因检测。使用生物信息学软件对病毒基因组开展分子遗传学分析。结果 2017年在贵州省德江县龙泉乡居池坝村、青龙镇石打头村、江口县快场村共采集蚊虫3属3种7 380只,其中骚扰阿蚊最多,占采集蚊虫总数的61.55%(4 542/7 380),中华按蚊占26.80%(1 978/7 380),三带喙库蚊810只以及其他蚊虫50只。从蚊虫中分离到3株引起组织培养细胞病变的病毒分离物;经分子生物学鉴定,从三带喙库蚊中分离的病毒株GZDJ1765属于Ⅰ型流行性乙型脑炎(乙脑)病毒,另2株病毒株均来自骚扰阿蚊(GZDJ1746-1、GZDJ1752-1),经鉴定为盖塔病毒。3批蚊虫(GZDJ1704、GZDJ1743-2、GZDJ1751-2)乙脑病毒基因PCR检测均为阳性。结论贵州省东北部地区所调查的农村地区动物饲养圈骚扰阿蚊为优势蚊种,蚊虫中携带乙脑病毒和盖塔病毒。
Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in northeastGuizhou province. Methods In 2017, host-seeking mosquitoes were collected from livestock sheds using Ultraviolet lightsin Dejiang and Jiangkou, Guizhou province. The mosquito specimens were classified according to morphology and stored inliquid nitrogen. All the samples were incubated with BHK-21 and C6/36 cells for virus isolation and detected the virus genes. The virus isolates were identified and analyzed by molecular biological methods. Results In total, there were 7 380 mosquitoes from 3 genera and 3 species collected from Dejiang and Jiangkou, Guizhou province, 61.55%(4 542/7 380) ofwhich were Armigeres subalbatus and 26.80%(1 978/7 380) were Anopheles sinensis. Out of the 3 virus strains isolated fromthe mosquitoes, One(GZDJ1765) was identified as type Ⅰ of Japanese encephalitis virus(JEV) isolated from Culex tritaeniorhynchus and two(GZDJ1746-1, GZDJ1752-1) were identified as Getah virus isolated from A. subalbatus. Threemosquito pools(GZDJ1704, GZDJ1743-2, GZDJ1751-2) tested positive for JEV genes y by PCR. Conclusion Armigeres subalbatus was the predominant species in northeast Guizhou. JEV and GETV were isolated.
Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in northeastGuizhou province. Methods In 2017, host-seeking mosquitoes were collected from livestock sheds using Ultraviolet lightsin Dejiang and Jiangkou, Guizhou province. The mosquito specimens were classified according to morphology and stored inliquid nitrogen. All the samples were incubated with BHK-21 and C6/36 cells for virus isolation and detected the virus genes. The virus isolates were identified and analyzed by molecular biological methods. Results In total, there were 7 380 mosquitoes from 3 genera and 3 species collected from Dejiang and Jiangkou, Guizhou province, 61.55%(4 542/7 380) ofwhich were Armigeres subalbatus and 26.80%(1 978/7 380) were Anopheles sinensis. Out of the 3 virus strains isolated fromthe mosquitoes, One(GZDJ1765) was identified as type Ⅰ of Japanese encephalitis virus(JEV) isolated from Culex tritaeniorhynchus and two(GZDJ1746-1, GZDJ1752-1) were identified as Getah virus isolated from A. subalbatus. Threemosquito pools(GZDJ1704, GZDJ1743-2, GZDJ1751-2) tested positive for JEV genes y by PCR. Conclusion Armigeres subalbatus was the predominant species in northeast Guizhou. JEV and GETV were isolated.
引文
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[21]赵春生,白志军,彭翼飞,等.我国南方人鼠虫媒病毒血清流行病学调查[J].中国公共卫生,2001,17(1):64-66. DOI:10.11847/zgggws2001-17-01-50.
[22] Li YY,Liu H,Fu SH,et al. From discovery to spread:the evolution and phylogeny of Getah virus[J]. Infect Genet Evol,2017,55:48-55. DOI:10.1016/j.meegid.2017.08.016.
[23] Yang T,Li R,Hu Y,et al. An outbreak of Getah virus infection among pigs in China,2017[J]. Transbound Emerg Dis,2018,65(3):632-637. DOI:10.1111/tbed.12867.
[2] Lei WW,Guo XF,Fu SH,et al. Correction:Isolation of Tibet orbivirus,TIBOV,from Culicoides Collected in Yunnan,China[J]. PLoS One,2015,10(9):e0139646. DOI:10.1371/journal.pone.0139646.
[3]李铭华,付士红,冯云,等.贵州省不同地区2008年虫媒病毒调查[J].中国媒介生物学及控制杂志,2012,23(5):417-420.
[4] Wang HY,Takasaki T,Fu SH,et al. Molecular epidemiological analysis of Japanese encephalitis virus in China[J]. J Gen Virol,2007,88(3):885-894. DOI:10.1099/vir.0.82185-0.
[5]叶绪芳,付士红,高晓艳,等.贵州省巴马森林病毒和罗斯河病毒感染的调查研究[J].中国媒介生物学及控制杂志,2012,23(5):428-431.
[6]王昭孝,武凤兰,唐光鹏.贵州地区人血清虫媒病毒抗体调查[J].预防医学情报杂志,1993,9增刊:129.
[7] Song S,Li YY,Fu SH,et al. Could Zika virus emerge in Mainland China? Virus isolation from nature in Culex quinquefasciatus,2016[J]. Emerg Microbes Infect,2017,6(11):e93. DOI:10.1038/emi.2017.80.
[8] Fu SH,Song S,Liu H,et al. Zika virus isolated from mosquitoes:a field and laboratory investigation in China,2016[J]. Sci China Life Sci,2017,60(12):1364-1371. DOI:10.1007/s11427-017-9196-8.
[9] Wang JL,Zhang HL,Sun XH,et al. Distribution of mosquitoes and mosquito-borne arboviruses in Yunnan province near the China-Myanmar-Laos border[J]. Am J Trop Med Hyg,2011,84(5):738-746. DOI:10.4269/ajtmh.2011.10-0294.
[10] Li YY,Fu SH,Guo XF,et al. Identification of a newly isolated Getah virus in the China-Laos border,China[J]. Biomed Environ Sci,2017,30(3):210-214. DOI:10.3967/bes2017.028.
[11]景晓,李兆凰,霍新北,等.骚扰阿蚊种群动态季节消长观察与分析[J].中国公共卫生,2004,20(8):936-937. DOI:10.11847/zgggws2004-20-08-24.
[12] Dick GWA. Zika virus(Ⅱ). Pathogenicity and physical properties[J]. T Roy Soc Trop Med Hyg,1952,46(5):521-534.DOI:10.1016/0035-9203(52)90043-6.
[13] Villar J,Ismail LC,Victora CG,et al. International standards for newborn weight,length,and head circumference by gestational ageandsex:theNewbornCross-Sectional studyof the INTERGROWTH-21st Project[J]. Lancet,2014,384(9946):857-868. DOI:10.1016/S0140-6736(14)60932-6.
[14] Nemoto M,Bannai H,Tsujimura K,et al. Getah virus infection among racehorses,Japan,2014[J]. Emerg Infect Dis,2015,21(5):883-885. DOI:10.3201/eid2105.141975.
[15]冯云,李胜国,张海林,等.云南腾冲县新分离乙型脑炎和盖塔病毒的分子生物学特征[J].中国人兽共患病学报,2014,30(4):353-357. DOI:10.3969/cjz.j.issn.1002-2694.2014.04.003.
[16]蒋廉华,赵春生.海南省1株甲属虫媒病毒的分离鉴定及其血清抗体调查[J].中国兽医学报,1999,19(6):549-551. DOI:10.16303/j.cnki.1005-4545.1999.06.009.
[17]王焕琴,刘卫滨,杨冬荣,等.河北省虫媒病毒分离鉴定[J].中华实验和临床病毒学杂志,2006,20(1):52-55. DOI:10.3760/cma.j.issn.1003-9279.2006.01.015.
[18] Zhai YG,Wang HY,Sun XH,et al. Complete sequence characterization of isolates of Getah virus(genus Alphavirus,family Togaviridae)from China[J]. J Gen Virol,2008,89(6):1446-1456. DOI:10.1099/vir.0.83607-0.
[19]翟友刚,王焕琴,许海魁,等.甘肃省天水及陇南部分地区虫媒病毒调查[J].中国人兽共患病学报,2008,24(2):95-99.DOI:10.3969/j.issn.1002-2694.2008.02.001.
[20]陈维欣,王环宇,付士红,等.我国新分离盖塔病毒的部分基因组分子特征研究[J].中华微生物学和免疫学杂志,2010,30(5):399-404. DOI:10.3760/cma.j.issn.0254-5101.2010.05.003.
[21]赵春生,白志军,彭翼飞,等.我国南方人鼠虫媒病毒血清流行病学调查[J].中国公共卫生,2001,17(1):64-66. DOI:10.11847/zgggws2001-17-01-50.
[22] Li YY,Liu H,Fu SH,et al. From discovery to spread:the evolution and phylogeny of Getah virus[J]. Infect Genet Evol,2017,55:48-55. DOI:10.1016/j.meegid.2017.08.016.
[23] Yang T,Li R,Hu Y,et al. An outbreak of Getah virus infection among pigs in China,2017[J]. Transbound Emerg Dis,2018,65(3):632-637. DOI:10.1111/tbed.12867.