转IbLEA 14基因杨树增强耐旱性的生理机制
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摘要
晚期胚胎蛋白富集蛋白(LEA)可作为多功能蛋白参与对多种非生物胁迫的响应。前期研究表明,过量表达甘薯IbLEA14基因能够增强转基因甘薯愈伤组织的耐渗透压和盐胁迫能力。为研究转IbLEA14基因杨树增强耐旱性的生理机制,本试验选取转IbLEA14基因杨树与非转基因杨树(NT)为材料,采用盆栽控水法,进行土壤干旱胁迫与复水处理,测定其形态与生理生化指标。结果表明:(1)干旱胁迫下,转IbLEA14基因杨树与NT株高增长、总生物量积累均受到抑制,但转基因杨树生长状况优于NT;与NT相比,转IbLEA14基因杨树叶片净光合速率较高,丙二醛(MDA)和过氧化氢(H2O2)含量较低,较高的脯氨酸(Pro)含量与较低的渗透势保障了转基因杨树较强的保水能力以及较高的叶片相对含水量。(2)复水后,较干旱条件下而言,转IbLEA14基因杨树叶片相对含水量增加,净光合速率升高,MDA和H2O2含量降低,Pro含量下降,渗透势升高。研究表明:转IbLEA14基因杨树可减轻干旱胁迫对植株细胞膜及光和系统的伤害,复水后能够更好地修复细胞膜系统与光合系统,加快恢复速度。因此,转IbLEA14基因杨树的耐旱性与旱后复水的恢复能力更好,更具有抗旱性优势。该研究结果将为杨树耐旱育种及应用提供理论依据,未来有望在改善土壤沙漠化方面发挥重要作用。
As a multifunctional protein, late embryogenesis abundant protein(LEA) participates in response to response multiple abiotic stresses. Previous studies have shown that overexpression of IbLEA14 gene in sweet potato could enhance the osmotic potential and salt stress tolerance in callus of transgenic sweet potato.In order tostudy how to improved the physiological mechanism of drought tolerance in IbLEA14 transgenic poplar, this study selected the transgenic poplar lines expressed IbLEA14 gene as material, used pot culture method and measured its morphological and physiological biochemical indexes under drought stress and rewatering. The results indicated:(1) Under drought stress, growth of plant height, total biomass accumulation of transgenic poplar expression IbLEA14 and NT poplar were all suppressed, but the growth condition of transgenic poplar was better than that of NT poplar, compared with NT poplar, the net photosynthesis rate of transgenic poplar was higher,while, MDA and H2 O2 content was lower, higher Pro content and lower osmotic potential to ensure the higher level of relative water content and water retention capacity of transgenic poplar.(2) Rewatering after drought stress,compared with the situation of drought stress, relative water content on the leaves of transgenic poplar were increased significantly,the net photosynthesis rate of transgenic poplar was gradually increased, the content of MDA and H2 O2 were sustain decreased, the content of proline was also reduced, and the osmotic potential was increased. The study showed that the transgenic poplar line expression IbLEA14 gene could reduce the damage of plant cell membrane and photosynthetic system under drought stress, when rewatering after drought stress, which could better to repair the cell membrane and photosynthetic apparatus, as well as accelerate the recovery.Therefore, transgenic poplar lines might have better drought tolerance and recovery ability after drought, and have more drought resistance advantages. The results of this study would provide a theoretical basis for drought resistance breeding and application of poplar drought tolerance, which would be expected to play an important role in improving soil desertification in the future.
As a multifunctional protein, late embryogenesis abundant protein(LEA) participates in response to response multiple abiotic stresses. Previous studies have shown that overexpression of IbLEA14 gene in sweet potato could enhance the osmotic potential and salt stress tolerance in callus of transgenic sweet potato.In order tostudy how to improved the physiological mechanism of drought tolerance in IbLEA14 transgenic poplar, this study selected the transgenic poplar lines expressed IbLEA14 gene as material, used pot culture method and measured its morphological and physiological biochemical indexes under drought stress and rewatering. The results indicated:(1) Under drought stress, growth of plant height, total biomass accumulation of transgenic poplar expression IbLEA14 and NT poplar were all suppressed, but the growth condition of transgenic poplar was better than that of NT poplar, compared with NT poplar, the net photosynthesis rate of transgenic poplar was higher,while, MDA and H2 O2 content was lower, higher Pro content and lower osmotic potential to ensure the higher level of relative water content and water retention capacity of transgenic poplar.(2) Rewatering after drought stress,compared with the situation of drought stress, relative water content on the leaves of transgenic poplar were increased significantly,the net photosynthesis rate of transgenic poplar was gradually increased, the content of MDA and H2 O2 were sustain decreased, the content of proline was also reduced, and the osmotic potential was increased. The study showed that the transgenic poplar line expression IbLEA14 gene could reduce the damage of plant cell membrane and photosynthetic system under drought stress, when rewatering after drought stress, which could better to repair the cell membrane and photosynthetic apparatus, as well as accelerate the recovery.Therefore, transgenic poplar lines might have better drought tolerance and recovery ability after drought, and have more drought resistance advantages. The results of this study would provide a theoretical basis for drought resistance breeding and application of poplar drought tolerance, which would be expected to play an important role in improving soil desertification in the future.
引文
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Chen S.C.,Wang M.,Wang J.,Gao Y.,Liu Z.Q.,and Wang X.,2017,Response of seed germination and seedling physiological characteristics of Medicago sativa to the simulated osmotic potential of PEG6000,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),28(9):2923-2931(陈士超,王猛,汪季,高永,刘宗奇,王香,2017,紫花苜蓿种子萌发及幼苗生理特性对PEG6000模拟渗透势的响应,应用生态学报,28(9):2923-2931)
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Fan R.P.,Zhou Q.,Zhou B.,and Jiang H.D.,2012,Effects of salinization stress on growth and the antioxidant system of tall fescue,Caoye Xuebao(Acta Prataculturae Sinica),21(1):112-117(樊瑞苹,周琴,周波,江海东,2012,盐胁迫对高羊茅生长及抗氧化系统的影响,草业学报,21(1):112-117)
Feng Y.L.,Zhang Y.J.,and Zhu C.Q.,2003,Relationship between photo-inhibition of photosynthesis and reactive oxygen species in leaves of poplars suffering root osmotic stress,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),14(8):1213-1217(冯玉龙,张亚杰,朱春全,2003,根系渗透胁迫时杨树光合作用光抑制与活性氧的关系,应用生态学报,14(8):1213-1217)
Hu X.J.,Ouyang X.,and Yu F.Y.,2010,Effects of drought stress on the growth and biomass of Pinus massoniana seedlings in different provenances,Jiangxi Nongye Daxue Xuebao(Acta Agriculturae Universitatis Jiangxiensis),32(3):510-516(胡晓健,欧阳献,喻方圆,2010,干旱胁迫对不同种源马尾松苗木生长及生物量的影响,江西农业大学学报,32(3):510-516)
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Kim S.H.,Ahn Y.O.,Ahn M.J.,Lee H.S.,and Kwak S.S.,2012,Down-regulation ofβ-carotene hydroxylase increasesβ-carotene and total carotenoids enhancing salt stress tolerance in transgenic cultured cells of sweetpotato,Phytochemistry,74:69-78
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Ashraf M.,and Foolad M.R.,2007,Roles of glycine betaine and proline in improving plant abiotic stress resistance,Environ.Exp.Bot.,59(2):206-216
Bao S.W.,2001,Effects of water stress on the state of water in Vicia faba L.leaves,Tianjin Shifan Daxue Xuebao(Journal of Tianjin Normal University(Natural Edition)),21(3):67-70(鲍思伟,2001,水分胁迫对蚕豆(Vicia faba L.)叶片水分状况的影响,天津师范大学学报(自然版),21(3):67-70)
Cai Z.Q.,Cao K.F.,Feng Y.L.,and Feng Z.L.,2003,Effect of low nocturnal temperature stress on fluorescence characteristics and active oxygen metabolism in leaves of Garcinia hanburyi seedlings grown under two levels of irradiance,Yingyong Shengtai Xuebao(Chinese Journal of Applied E-cology),14(3):326-330(蔡志全,曹坤芳,冯玉龙,冯志立,2003,夜间低温胁迫对两种生长光强下藤黄幼苗叶片荧光特征和活性氧代谢的影响,应用生态学报,14(3):326-330)
Chen S.,Shi M.T.,Wu Y.F.,and Li Y.Y.,2018,Effects of boron stress on active oxygen metabolism and antioxidant system of different genotypes of watermelon cultivars,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),34(4):60-64(陈晟,施木田,吴宇芬,李永裕,2018,硼胁迫对不同类型西瓜活性氧代谢与抗氧化系统的影响,中国农学通报,34(4):60-64)
Chen S.C.,Wang M.,Wang J.,Gao Y.,Liu Z.Q.,and Wang X.,2017,Response of seed germination and seedling physiological characteristics of Medicago sativa to the simulated osmotic potential of PEG6000,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),28(9):2923-2931(陈士超,王猛,汪季,高永,刘宗奇,王香,2017,紫花苜蓿种子萌发及幼苗生理特性对PEG6000模拟渗透势的响应,应用生态学报,28(9):2923-2931)
Cornic G.,2000,Drought stress inhibits photosynthesis by decreasing stomatal aperture-not by affecting ATP synthesis,Trends Plant Sci.,5(5):187-188
Cui X.M.,Liu X.B.,Li Z.H.,Sun K.Y.,Li H.,and Zhang T.T.,2012,Effects of salicylic acid on growth and photosynthetic characteristics of Melilotoides ruthenica in branching stage under different water stress,Caoye Xuebao(Acta Prataculturae Sinica),21(6):82-93(崔秀妹,刘信宝,李志华,孙凯燕,李卉,张婷婷,2012,水分胁迫下水杨酸对分枝期扁蓿豆生长及光合生理的影响,草业学报,21(6):82-93)
Ding L.,Li Y.R.,Li Y.,Shen Q.R.,and Guo S.W.,2014,Effects of drought stress on photosynthesis and water status of rice leaves,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),28(1):65-70(丁雷,李英瑞,李勇,沈其荣,郭世伟,2014,梯度干旱胁迫对水稻叶片光合和水分状况的影响,中国水稻科学,28(1):65-70)
Duan N.,Hao Y.G.,Liu F.,Xu J.,Chen H.L.,and Wang H.,2016,Study on water physiology of Nitraria billardieri in different provenances,Nanjing Nongye Xuebao(Southwest Agricultural Journal),29(5):1075-1080(段娜,郝玉光,刘芳,徐军,陈海玲,王珩,2016,不同种源比拉底白刺(Nitraria billardieri)水分生理研究,西南农业学报,29(5):1075-1080)
Fan R.P.,Zhou Q.,Zhou B.,and Jiang H.D.,2012,Effects of salinization stress on growth and the antioxidant system of tall fescue,Caoye Xuebao(Acta Prataculturae Sinica),21(1):112-117(樊瑞苹,周琴,周波,江海东,2012,盐胁迫对高羊茅生长及抗氧化系统的影响,草业学报,21(1):112-117)
Feng Y.L.,Zhang Y.J.,and Zhu C.Q.,2003,Relationship between photo-inhibition of photosynthesis and reactive oxygen species in leaves of poplars suffering root osmotic stress,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),14(8):1213-1217(冯玉龙,张亚杰,朱春全,2003,根系渗透胁迫时杨树光合作用光抑制与活性氧的关系,应用生态学报,14(8):1213-1217)
Hu X.J.,Ouyang X.,and Yu F.Y.,2010,Effects of drought stress on the growth and biomass of Pinus massoniana seedlings in different provenances,Jiangxi Nongye Daxue Xuebao(Acta Agriculturae Universitatis Jiangxiensis),32(3):510-516(胡晓健,欧阳献,喻方圆,2010,干旱胁迫对不同种源马尾松苗木生长及生物量的影响,江西农业大学学报,32(3):510-516)
Hundertmark M.,and Hincha D.K.,2008,LEA(late embryogenesis abundant)proteins and their encoding genes in Arabidopsis thaliana,BMC Genomics,9(1):118-140
Jia X.J.,Dong L.H.,Ding C.B.,Li X.,and Yuan M.,2013,Effects of drought stress on reactive oxygen species and their scavenging systems in Chlorophytum capense var.mediopictumleaf,Caoye Xuebao(Acta Prataculturae Sinica),22(5):248-255(贾学静,董立花,丁春邦,李旭,袁明,2013,干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响,草业学报,22(5):248-255)
Jing D.W.,Xing S.J.,Du Z.Y.,and Liu F.C.,2013,Effects of drought stress on the growth,photosynthetic characteristics,and active oxygen metabolism of poplar seedlings,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),24(7):1809-1816(井大炜,邢尚军,杜振宇,刘方春,2013,干旱胁迫对杨树幼苗生长、光合特性及活性氧代谢的影响,应用生态学报,24(7):1809-1816)
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