改性载体固定化石油降解菌的制备及降解性能
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摘要
以活性污泥中分离的石油降解菌为研究对象,通过物理吸附将其固定在不同天然载体上,研究天然载体及改性载体固定菌剂对石油废水的降解效果,探索制备固定化菌剂所需条件.结果表明,载体固定化菌种降解效果好于游离菌种降解效果,其中以凹凸棒石(ATP)固定化菌种降解效果最好,达到39.74%,比游离菌种降解效果提高了12%;对ATP进行不同方式改性,以先碱改后酸改的凹凸棒石(NH-ATP)降解效果最好(48.96%).在固定化时,每1g NH-ATP加入3.5g海藻酸钠作为粘合剂,固定化时间为21h,pH为7,温度为31℃,在浓度为1g/L的石油中,降解效果为60.54%,载体含菌量为1.29×1010CFU/g.
Taking the oil-degrading bacteria isolated from the activated sludge as the research object,and immobilized on different natural carriers by physical adsorption.The degradation of petroleum wastewater is studied by natural carrier and modified carrier,and the degradation conditions of petroleum wastewater were explored.The experimental results show that the degrading effect of immobilized bacteria is better than that of free bacteria,and the best degrading effect is attained by immobilized strains of attapulgite(39.74%),degradation effect than the free bacteria increased by 12%;ATP is modified by different ways,NH-ATP which is alkaline modification firstly and then acid-modified has the best effection(48.96%).3.5 g of sodium al-ginate was added in 1 g of NH-ATP as a binder during immobilization,the immobilization time is 21 h,the pH is 7 and the temperature is 31 ℃,in the oil with concentration of 1 g/L,the degradation effect is 60.54%,and the bacteria is 1.29×10~(10) CFU/g in the carrier.
Taking the oil-degrading bacteria isolated from the activated sludge as the research object,and immobilized on different natural carriers by physical adsorption.The degradation of petroleum wastewater is studied by natural carrier and modified carrier,and the degradation conditions of petroleum wastewater were explored.The experimental results show that the degrading effect of immobilized bacteria is better than that of free bacteria,and the best degrading effect is attained by immobilized strains of attapulgite(39.74%),degradation effect than the free bacteria increased by 12%;ATP is modified by different ways,NH-ATP which is alkaline modification firstly and then acid-modified has the best effection(48.96%).3.5 g of sodium al-ginate was added in 1 g of NH-ATP as a binder during immobilization,the immobilization time is 21 h,the pH is 7 and the temperature is 31 ℃,in the oil with concentration of 1 g/L,the degradation effect is 60.54%,and the bacteria is 1.29×10~(10) CFU/g in the carrier.
引文
[1]宋鹏.简述石油开采废水处理技术的现状与展望[J].中国新技术新产品,2012(1):199.SONG P.The present situation and prospect of the treatment technology of oil mining wastewater[J].China New Technologies and Products,2012(1):199.(in Chinese)
[2]曹云者,施烈焰,李丽和,等.石油烃污染场地环境风险评价与风险管理[J].生态毒理学报,2007,2(3):265-272.CAO Y Z,SHI L Y,LI L H,et al.Petroleum hydrocarbons-contaminated sites and related risk-based management strategy[J].Asian Journal of Ecotoxicoogy,2007,2(3):265-272.(in Chinese)
[3]郭璇,唐翠梅,赵春彦.浅谈国内石化废水深度处理回用技术应用现状[J].环境与可持续发展,2015,5(5):75-77.GUO X,TANG C M,ZHAO C Y.Shallowly discussed on the application status of petroleum chemical industry waste water adanced treatment and reuse technology[J].Environment and Sustainbale Development,2015,5(5):75-77.(in Chinese)
[4]秦胜东,郭嘉昒,刘玉存,等.固定化微生物技术研究进展及其在水处理中的应用[J].水处理技术,2014,40(10):6-11.QIN S D,GUO J H,LIU Y C,et al.Research progress in immobilized microorganism technology and its application in water treatment[J].Technology of Water Treatment,2014,40(10):6-11.(in Chinese)
[5]王洪祚,刘世勇.酶和细胞的固定化[J].化学通报,1997,6(2):22-27.WANG H Z,LIU S Y.Enzyme and cell immobilization[J].Chemistry Bulletin,1997,6(2):25-30.(in Chinese)
[6]BANERJEE A,GHOSHAL AK.Phenol degradation by Bacillus cereus:Pathway and kinetic modeling[J].Bioresource Technology,2010,101(14):5501-5507.
[7]高祥兴,高伟,崔志松,等.海洋石油降解菌群的固定化及石油降解特性[J].海洋与湖沼,2015,46(4):732-740.GAO X X,GAO W,CUI Z S,et al.Immobilization of marine oil-degradating Bacterial consortiun and its degrdation characteristics[J].Oceanologia et Limnologia Sinica,2015,46(4):732-740.(in Chinese)
[8]詹龙辉,冼萍,许桂苹,等.固定化微生物深度降解垃圾渗滤液氨氮[J].环境工程学报,2017,11(3):1482-1486.ZHAN L H,XIAN P,XU G P,et al.Ammonia nitrogen advanced removal of landfill leachate by immobilized micro-organism[J].Chinese Journal of Environmental Engineering,2017,11(3):1482-1486.(in Chinese)
[9]陶柯霖,贾汉忠,汪立今,等.高岭石固定化微生物对菲的降解研究[J].应用化工,2017,46(4):650-654.TAO K L,JIA H Z,WANG L J,et al.Biodegradation of phenanthrene by kaolinite immobilized a degradation bacteria[J].Applied Chemical Industry,2017,46(4):650-654.(in Chinese)
[10]张闻,赵延君,王加宁,等.生物炭固定化石油降解菌剂的制备[J].江苏农业科学,2015,43(6):341-345.ZHANG W,ZHAO Y J,WANG J N,et al.Preparation of biochar immobilized microbial oil degrading microorganisms[J].Jiangsu Agricultural Sciences,2015,43(6):341-345.(in Chinese)
[11]包木太,田艳敏,陈庆国.海藻酸钠包埋固定化微生物处理含油废水研究[J].环境科学与技术,2012,35(2):167-172.BAO M T,TIAN Y M,CHEN Q G.Application of alginate embedded immobilized microbes treatment oily wastewater treatment[J].Environmental Science and Technology,2012,35(2):167-172.(in Chinese)
[12]于鲁冀,李廷梅,杨强,等.亚硝化混合菌的固定化及去除河水NH3-N影响因素[J].环境工程学报,2014,8(8):3154-3160.YU L J,LI T M,YANG Q,et al.Immobilization of mixed nitrosation bacteria and influencing factors of NH3-N removal in river water[J].Chinese Journal of Environmental Engineering,2014,8(8):3154-3160.(in Chinese)
[13]柴琴琴,呼世斌,刘建伟,等.有机改性凹凸棒石对养猪废水中有机物的吸附研究[J].环境科学学报,2016,36(5):1672-1682.CHAI Q Q,HU S B,LIU J W,et al.The adsorption of organics in swine wastewater by organo-attapulgite[J].Acta Scientiae Circumstantiae,2016,36(5):1672-1682.(in Chinese)
[14]彭进平,赖焕然,程高,等.改性硅藻土的制备、表征及其在富营养化水体除磷中的应用[J].生态环境学报,2010,19(8):1936-1940.PENG J P,LAI H R,CHENG G,et al.The preparation,characterization and application of the modified diatomite[J].Ecology and Environmental Scences,2010,19(8):1936-1940.(in Chinese)
[15]陈天虎,汪家权.蒙脱石粘土改性吸附剂处理印染废水实验研究[J].中国环境科学,1996,16(1):60-63.CHEN T H,WANG J Q.Experimental study on treating printing and dyeing wastewater with the modified adsorbent of montmorillonite clay[J].China Environmental Science,1996,16(1):60-63.(in Chinese)
[16]TAO X Q,LU G N,DANG Z,et al.A phenanthrene degrading strain Spingomonas sp.GY2Bisolated from contaminated soils[J].Process Biochemistry,2007,42(3):401-408.
[17]徐新阳,谷妮娜.含油废水处理微生物的化学包埋法固定化工艺[J].东北大学学报(自然科学版),2007,28(9):1329-1332.XU X Y,GU N N.Chemical embedding Immobilization technique of microorganism used in oil contaminated wastewater remediation[J].Journal of Northeastern University(Natural Science),2007,28(9):1329-1332.(in Chinese)
[18]姚磊,叶正芳,倪晋仁,等.固定化曝气生物滤池处理污染河水的中试研究[J].中国给水排水,2007,23(13):20-23.YAO L,YE Z F,NI J R,et al.Pilot-scale study on polluted river water treatment by G-BAF[J].China water and wastewater,2007,23(13):20-23.(in Chinese)
[19]HUANG J H,WANG X G,JIN Q Z,et al.Adsorption of phenol on organic modified attapulgite by ultrasonic method[J].Techniques and Equipment for Environmental Pollution Control,2005,6(9):25-28.
[20]YAO C,LIU M,LI W M,et al.Adsorption capability of methylene blue by attapulgite/zinc oxide nanocomposites[J].Acta Scientiae Circumstantiae,2010,30(6):1211-1219.
[21]刘国良,苏幼明,顾书敏,等.石油污染土壤生物修复研究新进展[J].化学与生物工程,2008,25(8):1-4.LIU G L,SU Y M,GU S M,et al.New progress in bioremediation of petroleum-contaminated soil[J].Chemistry and Bioengineering,2008,25(8):1-4.(in Chinese)
[2]曹云者,施烈焰,李丽和,等.石油烃污染场地环境风险评价与风险管理[J].生态毒理学报,2007,2(3):265-272.CAO Y Z,SHI L Y,LI L H,et al.Petroleum hydrocarbons-contaminated sites and related risk-based management strategy[J].Asian Journal of Ecotoxicoogy,2007,2(3):265-272.(in Chinese)
[3]郭璇,唐翠梅,赵春彦.浅谈国内石化废水深度处理回用技术应用现状[J].环境与可持续发展,2015,5(5):75-77.GUO X,TANG C M,ZHAO C Y.Shallowly discussed on the application status of petroleum chemical industry waste water adanced treatment and reuse technology[J].Environment and Sustainbale Development,2015,5(5):75-77.(in Chinese)
[4]秦胜东,郭嘉昒,刘玉存,等.固定化微生物技术研究进展及其在水处理中的应用[J].水处理技术,2014,40(10):6-11.QIN S D,GUO J H,LIU Y C,et al.Research progress in immobilized microorganism technology and its application in water treatment[J].Technology of Water Treatment,2014,40(10):6-11.(in Chinese)
[5]王洪祚,刘世勇.酶和细胞的固定化[J].化学通报,1997,6(2):22-27.WANG H Z,LIU S Y.Enzyme and cell immobilization[J].Chemistry Bulletin,1997,6(2):25-30.(in Chinese)
[6]BANERJEE A,GHOSHAL AK.Phenol degradation by Bacillus cereus:Pathway and kinetic modeling[J].Bioresource Technology,2010,101(14):5501-5507.
[7]高祥兴,高伟,崔志松,等.海洋石油降解菌群的固定化及石油降解特性[J].海洋与湖沼,2015,46(4):732-740.GAO X X,GAO W,CUI Z S,et al.Immobilization of marine oil-degradating Bacterial consortiun and its degrdation characteristics[J].Oceanologia et Limnologia Sinica,2015,46(4):732-740.(in Chinese)
[8]詹龙辉,冼萍,许桂苹,等.固定化微生物深度降解垃圾渗滤液氨氮[J].环境工程学报,2017,11(3):1482-1486.ZHAN L H,XIAN P,XU G P,et al.Ammonia nitrogen advanced removal of landfill leachate by immobilized micro-organism[J].Chinese Journal of Environmental Engineering,2017,11(3):1482-1486.(in Chinese)
[9]陶柯霖,贾汉忠,汪立今,等.高岭石固定化微生物对菲的降解研究[J].应用化工,2017,46(4):650-654.TAO K L,JIA H Z,WANG L J,et al.Biodegradation of phenanthrene by kaolinite immobilized a degradation bacteria[J].Applied Chemical Industry,2017,46(4):650-654.(in Chinese)
[10]张闻,赵延君,王加宁,等.生物炭固定化石油降解菌剂的制备[J].江苏农业科学,2015,43(6):341-345.ZHANG W,ZHAO Y J,WANG J N,et al.Preparation of biochar immobilized microbial oil degrading microorganisms[J].Jiangsu Agricultural Sciences,2015,43(6):341-345.(in Chinese)
[11]包木太,田艳敏,陈庆国.海藻酸钠包埋固定化微生物处理含油废水研究[J].环境科学与技术,2012,35(2):167-172.BAO M T,TIAN Y M,CHEN Q G.Application of alginate embedded immobilized microbes treatment oily wastewater treatment[J].Environmental Science and Technology,2012,35(2):167-172.(in Chinese)
[12]于鲁冀,李廷梅,杨强,等.亚硝化混合菌的固定化及去除河水NH3-N影响因素[J].环境工程学报,2014,8(8):3154-3160.YU L J,LI T M,YANG Q,et al.Immobilization of mixed nitrosation bacteria and influencing factors of NH3-N removal in river water[J].Chinese Journal of Environmental Engineering,2014,8(8):3154-3160.(in Chinese)
[13]柴琴琴,呼世斌,刘建伟,等.有机改性凹凸棒石对养猪废水中有机物的吸附研究[J].环境科学学报,2016,36(5):1672-1682.CHAI Q Q,HU S B,LIU J W,et al.The adsorption of organics in swine wastewater by organo-attapulgite[J].Acta Scientiae Circumstantiae,2016,36(5):1672-1682.(in Chinese)
[14]彭进平,赖焕然,程高,等.改性硅藻土的制备、表征及其在富营养化水体除磷中的应用[J].生态环境学报,2010,19(8):1936-1940.PENG J P,LAI H R,CHENG G,et al.The preparation,characterization and application of the modified diatomite[J].Ecology and Environmental Scences,2010,19(8):1936-1940.(in Chinese)
[15]陈天虎,汪家权.蒙脱石粘土改性吸附剂处理印染废水实验研究[J].中国环境科学,1996,16(1):60-63.CHEN T H,WANG J Q.Experimental study on treating printing and dyeing wastewater with the modified adsorbent of montmorillonite clay[J].China Environmental Science,1996,16(1):60-63.(in Chinese)
[16]TAO X Q,LU G N,DANG Z,et al.A phenanthrene degrading strain Spingomonas sp.GY2Bisolated from contaminated soils[J].Process Biochemistry,2007,42(3):401-408.
[17]徐新阳,谷妮娜.含油废水处理微生物的化学包埋法固定化工艺[J].东北大学学报(自然科学版),2007,28(9):1329-1332.XU X Y,GU N N.Chemical embedding Immobilization technique of microorganism used in oil contaminated wastewater remediation[J].Journal of Northeastern University(Natural Science),2007,28(9):1329-1332.(in Chinese)
[18]姚磊,叶正芳,倪晋仁,等.固定化曝气生物滤池处理污染河水的中试研究[J].中国给水排水,2007,23(13):20-23.YAO L,YE Z F,NI J R,et al.Pilot-scale study on polluted river water treatment by G-BAF[J].China water and wastewater,2007,23(13):20-23.(in Chinese)
[19]HUANG J H,WANG X G,JIN Q Z,et al.Adsorption of phenol on organic modified attapulgite by ultrasonic method[J].Techniques and Equipment for Environmental Pollution Control,2005,6(9):25-28.
[20]YAO C,LIU M,LI W M,et al.Adsorption capability of methylene blue by attapulgite/zinc oxide nanocomposites[J].Acta Scientiae Circumstantiae,2010,30(6):1211-1219.
[21]刘国良,苏幼明,顾书敏,等.石油污染土壤生物修复研究新进展[J].化学与生物工程,2008,25(8):1-4.LIU G L,SU Y M,GU S M,et al.New progress in bioremediation of petroleum-contaminated soil[J].Chemistry and Bioengineering,2008,25(8):1-4.(in Chinese)