光照和温度对红花槭限制生长保存的影响
|
摘要
该文主要探讨了光照时间、光照强度、温度及昼夜温差等保存条件对卓越红花槭(Acer rubrum cv.‘Somerset’)限制生长保存的影响。结果表明,在为期182天的保存过程中,离体材料前期以分化生长为主,后期以营养生长为主,并呈现一定的低温适应性。温度对离体材料生长的影响达极显著水平(P<0.01);光照时间和光照强度影响持久,二者交互作用达显著水平(P<0.05);昼夜温差对平均出芽数和生根率都有显著影响。研究表明,保存效果最好的条件是T3(25°C,12小时光照,62.50μmol·m~(–2)·s~(–1))和T7(25°C,12小时光照,31.25μmol·m~(–2)·s~(–1))处理组,但最佳保存条件的选择标准并不唯一,其核心是保证种质材料的分化能力。
We examined the effect of conservation conditions on restricting the conservation of Acer rubrum L. cv. ‘Somerset', including photoperiod, light intensity, temperature and diurnal temperature variation. In the 182-day conservation period, the in vitro materials were mainly for differentiation in the early period and vegetative growth in the later period, also gradually adapted to the low-temperature environment. The effect of temperature on the growth of in vitro materials reached extremely significant level(P<0.01); the effect of photoperiod and light intensity lasted for a long time, and their interaction reached significant level(P<0.05). Diurnal temperature variation had a significant effect on average number of shoots and rate of rooting. In all of the tests, 25°C, illumination 12 h, 62.50 μmol·m~(–2)·s~(–1) and 25°C, illumination 12 h, 31.25 μmol·m~(–2)·s~(–1) conditions were both able to restrict conservation. The selection criteria for the best conservation conditions was not unique, and the core was keeping the differentiation ability of the in vitro materials.
We examined the effect of conservation conditions on restricting the conservation of Acer rubrum L. cv. ‘Somerset', including photoperiod, light intensity, temperature and diurnal temperature variation. In the 182-day conservation period, the in vitro materials were mainly for differentiation in the early period and vegetative growth in the later period, also gradually adapted to the low-temperature environment. The effect of temperature on the growth of in vitro materials reached extremely significant level(P<0.01); the effect of photoperiod and light intensity lasted for a long time, and their interaction reached significant level(P<0.05). Diurnal temperature variation had a significant effect on average number of shoots and rate of rooting. In all of the tests, 25°C, illumination 12 h, 62.50 μmol·m~(–2)·s~(–1) and 25°C, illumination 12 h, 31.25 μmol·m~(–2)·s~(–1) conditions were both able to restrict conservation. The selection criteria for the best conservation conditions was not unique, and the core was keeping the differentiation ability of the in vitro materials.
引文
兰伟,陈发棣(2010).植物种质资源缓慢生长法保存研究进展.阜阳师范学院学报(自然科学版)27(2),68-72.
刘静波,林士杰,张忠辉,谢朋,蔡群,张大伟,勾天兵,赵珊珊,赵洪伟,王志强(2012).槭属植物种质资源研究进展.中国农学通报28(25),1-5.
任桂萍,王小菁,朱根发(2016).不同光质的LED对蝴蝶兰组织培养增殖及生根的影响.植物学报51,81-88.
咸洋,吴丹,解孝满,刘德深,韩彪,田莎莎,刘丹(2014).一种红花槭的组培快繁方法.中国专利,201310493738.1.2014-02-05.
徐呈祥(2012).提高植物抗寒性的机理研究进展.生态学报32,7966-7980.
徐刚标(2000).植物种质资源离体保存研究进展.中南林学院学报20(4),81-87.
燕丽萍,李丽,刘翠兰,吴德军,王因花,任飞,赵梁军(2016).绒毛白蜡体胚诱导和植株再生.植物学报51,807-816.
杨博,曹秀婷,王政,何松林(2012).昼夜温差对非洲菊试管苗生长的影响.西北林学院学报27(2),88-92.
张红岩,吴小芹,朱丽华,谈家金(2010).抗病赤松组培丛生芽低温保存条件研究.南京林业大学学报(自然科学版)34(5),7-11.
郑卫杰,郭子霞,王政,何松林(2011).昼夜温差对文心兰试管苗生长的影响.西北林学院学报26(4),137-141.
周逊,向长萍(2008).植物种质资源缓慢生长离体保存研究进展.中国蔬菜(11),39-42.
Ahmad N,Abbasi BH,Fazal H,Khan MA,Afridi MS(2014).Effect of reverse photoperiod on in vitro regeneration and piperine production in Piper nigrum L.C R Biologies 337,19-28.
Jouve L,Franck T,Gaspars T,Cattivelli L,Hausman JF(2000).Poplar acclimation to cold during in vitro conservation at low non-freezing temperature:metabolic and proteic changes.J Plant Physiol 157,117-123.
Kozai T,Watanabe K,Jeong BR(1995).Stem elongation and growth of Solanum tuberosum L.in vitro in response to photosynthetic photon flux,photoperiod and difference in photoperiod and dark period temperatures.Sci Hortic64,1-9.
Salcedo-Morales G,Rosas-Romero G,Nabor-Correa N,Bermúdez-Torres K,López-Laredo AR,Trejo-Tapia G(2009).Propagation and conservation of Castilleja tenuiflora Benth.Polibotánica(28),119-137.
Sarasan V,Cripps R,Ramsay MM,Atherton C,Mcmichen M,Prendergast G,Rowntree JK(2006).Conservation in vitro of threatened plants-progress in the past decade.In Vitro Cell Dev Biol Plant 42,206-214.
Silveira DG,Souza FVD,Pelacani CR,da Silva Souza A,da Silva Ledo CA,de Santana JRF(2009).Micropropagation and in vitro conservation of Neoglaziovia variegata(Arr.Cam.)Mez,a fiber producing bromeliad from Brazil.Braz Arch Biol Technol 52,923-932.
刘静波,林士杰,张忠辉,谢朋,蔡群,张大伟,勾天兵,赵珊珊,赵洪伟,王志强(2012).槭属植物种质资源研究进展.中国农学通报28(25),1-5.
任桂萍,王小菁,朱根发(2016).不同光质的LED对蝴蝶兰组织培养增殖及生根的影响.植物学报51,81-88.
咸洋,吴丹,解孝满,刘德深,韩彪,田莎莎,刘丹(2014).一种红花槭的组培快繁方法.中国专利,201310493738.1.2014-02-05.
徐呈祥(2012).提高植物抗寒性的机理研究进展.生态学报32,7966-7980.
徐刚标(2000).植物种质资源离体保存研究进展.中南林学院学报20(4),81-87.
燕丽萍,李丽,刘翠兰,吴德军,王因花,任飞,赵梁军(2016).绒毛白蜡体胚诱导和植株再生.植物学报51,807-816.
杨博,曹秀婷,王政,何松林(2012).昼夜温差对非洲菊试管苗生长的影响.西北林学院学报27(2),88-92.
张红岩,吴小芹,朱丽华,谈家金(2010).抗病赤松组培丛生芽低温保存条件研究.南京林业大学学报(自然科学版)34(5),7-11.
郑卫杰,郭子霞,王政,何松林(2011).昼夜温差对文心兰试管苗生长的影响.西北林学院学报26(4),137-141.
周逊,向长萍(2008).植物种质资源缓慢生长离体保存研究进展.中国蔬菜(11),39-42.
Ahmad N,Abbasi BH,Fazal H,Khan MA,Afridi MS(2014).Effect of reverse photoperiod on in vitro regeneration and piperine production in Piper nigrum L.C R Biologies 337,19-28.
Jouve L,Franck T,Gaspars T,Cattivelli L,Hausman JF(2000).Poplar acclimation to cold during in vitro conservation at low non-freezing temperature:metabolic and proteic changes.J Plant Physiol 157,117-123.
Kozai T,Watanabe K,Jeong BR(1995).Stem elongation and growth of Solanum tuberosum L.in vitro in response to photosynthetic photon flux,photoperiod and difference in photoperiod and dark period temperatures.Sci Hortic64,1-9.
Salcedo-Morales G,Rosas-Romero G,Nabor-Correa N,Bermúdez-Torres K,López-Laredo AR,Trejo-Tapia G(2009).Propagation and conservation of Castilleja tenuiflora Benth.Polibotánica(28),119-137.
Sarasan V,Cripps R,Ramsay MM,Atherton C,Mcmichen M,Prendergast G,Rowntree JK(2006).Conservation in vitro of threatened plants-progress in the past decade.In Vitro Cell Dev Biol Plant 42,206-214.
Silveira DG,Souza FVD,Pelacani CR,da Silva Souza A,da Silva Ledo CA,de Santana JRF(2009).Micropropagation and in vitro conservation of Neoglaziovia variegata(Arr.Cam.)Mez,a fiber producing bromeliad from Brazil.Braz Arch Biol Technol 52,923-932.