3种赤眼蜂对二化螟和稻纵卷叶螟性信息素的嗅觉反应
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摘要
为明确3种稻田常见赤眼蜂,稻螟赤眼蜂Trichogramma japonicun Ashmead、螟黄赤眼蜂T.chilonis Ishii和松毛虫赤眼蜂T.dendrolimi Matsumura对水稻主要鳞翅目害虫二化螟Chilo suppressalis(Walker)和稻纵卷叶螟Cnaphalocrocis medinalis(Guenée)人工合成性信息素的反应,本研究采用Y-型嗅觉仪测定了赤眼蜂对0.01、0.1、1、10、100和1000μg 6种不同剂量性信息素诱芯的嗅觉反应。结果表明,二化螟性信息素对稻螟赤眼蜂雌蜂具有显著的吸引作用,对螟黄赤眼蜂和松毛虫赤眼蜂的吸引作用不明显。而稻纵卷叶螟性信息素对3种赤眼雌蜂仅在特定浓度下具有显著的吸引作用。这说明赤眼蜂可以利用寄主的性信息素对寄主进行定位。利用人工合成性信息素对赤眼蜂吸引力的特性可用以促进非稻田生境中的赤眼蜂向稻田迁移从而提高稻田的生物防治能力。
Responses of three Trichogramma species, Trichogramma japonicun, T. chilonis and T. dendrolimi, in rice ecosystem to synthetic sex pheromone of two main Lepidoptera pests, stripped stem borer Chilo suppressalis(Walker)and rice leaf folder Cnaphalocrocis medinalis(Guenée)were determined using the Y-tube olfactometer. The concentrations of sex pheromone were 0.01, 0.1, 1, 10, 100 and 1000 μg. The results showed that the sex pheromone lure of C. suppressalis significantly attracted T. japonicun females, but not significantly attracted T. chilonis females and T. dendrolimi females. The sex pheromone lure of C. suppressalis significantly attracted T. japonicum females, T. chilonis females and T. dendrolimi females only under certain concentration. Those results indicate that the tested Trichogramma species can use host sex pheromone as a cue to search hosts, which may promote Trichogramma species' migration from non-rice habit to rice ecosystem and improve biological control of lepidopteran pests.
Responses of three Trichogramma species, Trichogramma japonicun, T. chilonis and T. dendrolimi, in rice ecosystem to synthetic sex pheromone of two main Lepidoptera pests, stripped stem borer Chilo suppressalis(Walker)and rice leaf folder Cnaphalocrocis medinalis(Guenée)were determined using the Y-tube olfactometer. The concentrations of sex pheromone were 0.01, 0.1, 1, 10, 100 and 1000 μg. The results showed that the sex pheromone lure of C. suppressalis significantly attracted T. japonicun females, but not significantly attracted T. chilonis females and T. dendrolimi females. The sex pheromone lure of C. suppressalis significantly attracted T. japonicum females, T. chilonis females and T. dendrolimi females only under certain concentration. Those results indicate that the tested Trichogramma species can use host sex pheromone as a cue to search hosts, which may promote Trichogramma species' migration from non-rice habit to rice ecosystem and improve biological control of lepidopteran pests.
引文
[1]肖海军,何海敏,薛芳森.二化螟滞育生物学特性的研究进展[J].生物灾害科学,2012,35(1):1-6.
[2]鲁艳辉,赵燕燕,张发成,等.二化螟滞育生物钟蛋白TIME-EA4基因的克隆及时空和温度诱导表达分析[J].昆虫学报,2016,59(4):392-401.
[3]吕仲贤主编.稻纵卷叶螟绿色防控彩色图谱[M].北京:中国农业出版社,2017.
[4]杨亚军,徐红星,郑许松,等.中国稻纵卷叶螟防控技术进展[J].植物保护学报,2015,42(5):691-701.
[5]Yao R,Zhao D D,Zhang S,et al.Monitoring and mechanisms of insecticide resistance in Chilo suppressalis(Lepidoptera:Crambidae)with special reference to diamides[J].Pest Management Science,2016,76(6):1169-1178.
[6]Romeis J,Babendreier D,W?ckers F L,et al.Habitat and plant specificity of Trichogramma,egg parasitoids-underlying mechanisms and implications[J].Basic and Applied Ecology,2005,6(3):215-236.
[7]刘树生,施祖华.赤眼蜂研究和应用进展[J].中国生物防治学报,1996,12(2):78-84.
[8]Hilker M,Meiners T.Induction of plant responses to oviposition and feeding by herbivorous arthropods:a comparison[J].Entomologia Experimentalis etApplicata,2002,104(1):181-192.
[9]Mcneil J N.Behavioral ecology of pheromone-mediated communication in noths and its importance in the use of phermone traps[J].Annual Review of Entomology,1991,36:407-430.
[10]白树雄,王振营,何康来.信息化合物对寄生蜂寄主定向与定位行为的调控[J].中国生物防治学报,2001,17(2):86-91.
[11]Borges M,Mlm C,Sujii E R,et al.Semiochemical and physical stimuli involved in host recognition by Telenomus podisi(Hymenoptera:Scelionidae)toward Euschistus heros(Heteroptera:Pentatomidae)[J].Physiological Entomology,1999,24(3):227-233.
[12]Reddy G V,Holopainen J K,Guerrero A.Olfactory responses of Plutella xylostella natural enemies to host pheromone,larval frass,and green leaf cabbage volatiles[J].Journal of Chemical Ecology,2002,28(1):131-143.
[13]朱平阳.显花植物对稻飞虱卵期主要天敌的生态功能[D].浙江:浙江师范大学,2012.
[14]Vet L E M,Dicke M.Ecology of infochemical use by natural enemies in a tritrophic context[J].Annual Review of Entomology,1992,37(1):141-172.
[15]Mattiacci L,Hütter E,Dorn S.Host location of Hyssopus pallidus,a larval parasitoid of the codling moth,Cydia pomonella[J].Biological Control,1999,15(3):241-251.
[16]Santolamazza-Carbone S,Rodr??Guez-Illamola A,Rivera A C.Host finding and host discrimination ability in Anaphes nitens Girault,an egg parasitoid of the Eucalyptus,snout-beetle Gonipterus scutellatus Gyllenhal[J].Biological Control,2004,29(1):24-33.
[17]练永国,王素琴,白树雄,等.挥发性信息化合物对玉米螟赤眼蜂寄主选择行为的影响[J].昆虫学报,2007,50(5):448-453.
[18]Noldus L P J J.Response of the egg parasitoid Trichogramma pretiosum,to the sex pheromone of its host Heliothis zea[J].Entomologia Experimentalis et Applicata,1988,48(3):293-300.
[19]Mcgregor R,Henderson D.The influence of oviposition experience on response to host pheromone in Trichogramma sibericum(Hymenoptera:Trichogrammatidae)[J].Journal of Insect Behavior,1998,11(5):621-632.
[20]Reddy G V,Guerrero A.Behavioral responses of the diamondback moth,Plutella xylostella,to green leaf volatiles of Brassica oleracea subsp.capitata[J].Journal of Agricultural and Food Chemistry,2001,48(12):6025-6029.
[21]Colazza S,Rosi M C,Clemente A.Response of egg parasitoid Telenomus busseolae,to sex pheromone of Sesamia nonagrioides[J].Journal of Chemical Ecology,1997,23(11):2437-2444.
[22]Renou M,Nagnan P,Berthier A,et al.Identification of compounds from the eggs of Ostrinia nubilalis,and Mamestra brassicae,having kairomone activity on Trichogramma brassicae[J].Entomologia Experimentalis et Applicata,1992,63(3):291-303.
[23]白树雄,王振营,何康来,等.玉米螟赤眼蜂对亚洲玉米螟益它素的嗅觉反应[J].昆虫学报,2004,47(1):48-54.
[24]Lewis W J,Nordlund D A,Gueldner R C,et al.Kairomones and their use for management of entomophagous insects:XIII.Kairomonal activity for Trichogramma spp.of abdominal tips,excretion,and a synthetic sex pheromone blend of Heliothis zea(Boddie)moths[J].Journal of Chemical Ecology,1981,3(6):513-518.
[25]Arakaki N,Wakamura S,Yasuda T.Phoretic egg parasitoid,Telenomus euproctidis(Hymenoptera:Scelionidae),uses sex pheromone of tussock moth Euproctis taiwana(Lepidoptera:Lymantriidae)as a kairomone[J].Journal of Chemical Ecology,1996,22(6):1079-1085.
[2]鲁艳辉,赵燕燕,张发成,等.二化螟滞育生物钟蛋白TIME-EA4基因的克隆及时空和温度诱导表达分析[J].昆虫学报,2016,59(4):392-401.
[3]吕仲贤主编.稻纵卷叶螟绿色防控彩色图谱[M].北京:中国农业出版社,2017.
[4]杨亚军,徐红星,郑许松,等.中国稻纵卷叶螟防控技术进展[J].植物保护学报,2015,42(5):691-701.
[5]Yao R,Zhao D D,Zhang S,et al.Monitoring and mechanisms of insecticide resistance in Chilo suppressalis(Lepidoptera:Crambidae)with special reference to diamides[J].Pest Management Science,2016,76(6):1169-1178.
[6]Romeis J,Babendreier D,W?ckers F L,et al.Habitat and plant specificity of Trichogramma,egg parasitoids-underlying mechanisms and implications[J].Basic and Applied Ecology,2005,6(3):215-236.
[7]刘树生,施祖华.赤眼蜂研究和应用进展[J].中国生物防治学报,1996,12(2):78-84.
[8]Hilker M,Meiners T.Induction of plant responses to oviposition and feeding by herbivorous arthropods:a comparison[J].Entomologia Experimentalis etApplicata,2002,104(1):181-192.
[9]Mcneil J N.Behavioral ecology of pheromone-mediated communication in noths and its importance in the use of phermone traps[J].Annual Review of Entomology,1991,36:407-430.
[10]白树雄,王振营,何康来.信息化合物对寄生蜂寄主定向与定位行为的调控[J].中国生物防治学报,2001,17(2):86-91.
[11]Borges M,Mlm C,Sujii E R,et al.Semiochemical and physical stimuli involved in host recognition by Telenomus podisi(Hymenoptera:Scelionidae)toward Euschistus heros(Heteroptera:Pentatomidae)[J].Physiological Entomology,1999,24(3):227-233.
[12]Reddy G V,Holopainen J K,Guerrero A.Olfactory responses of Plutella xylostella natural enemies to host pheromone,larval frass,and green leaf cabbage volatiles[J].Journal of Chemical Ecology,2002,28(1):131-143.
[13]朱平阳.显花植物对稻飞虱卵期主要天敌的生态功能[D].浙江:浙江师范大学,2012.
[14]Vet L E M,Dicke M.Ecology of infochemical use by natural enemies in a tritrophic context[J].Annual Review of Entomology,1992,37(1):141-172.
[15]Mattiacci L,Hütter E,Dorn S.Host location of Hyssopus pallidus,a larval parasitoid of the codling moth,Cydia pomonella[J].Biological Control,1999,15(3):241-251.
[16]Santolamazza-Carbone S,Rodr??Guez-Illamola A,Rivera A C.Host finding and host discrimination ability in Anaphes nitens Girault,an egg parasitoid of the Eucalyptus,snout-beetle Gonipterus scutellatus Gyllenhal[J].Biological Control,2004,29(1):24-33.
[17]练永国,王素琴,白树雄,等.挥发性信息化合物对玉米螟赤眼蜂寄主选择行为的影响[J].昆虫学报,2007,50(5):448-453.
[18]Noldus L P J J.Response of the egg parasitoid Trichogramma pretiosum,to the sex pheromone of its host Heliothis zea[J].Entomologia Experimentalis et Applicata,1988,48(3):293-300.
[19]Mcgregor R,Henderson D.The influence of oviposition experience on response to host pheromone in Trichogramma sibericum(Hymenoptera:Trichogrammatidae)[J].Journal of Insect Behavior,1998,11(5):621-632.
[20]Reddy G V,Guerrero A.Behavioral responses of the diamondback moth,Plutella xylostella,to green leaf volatiles of Brassica oleracea subsp.capitata[J].Journal of Agricultural and Food Chemistry,2001,48(12):6025-6029.
[21]Colazza S,Rosi M C,Clemente A.Response of egg parasitoid Telenomus busseolae,to sex pheromone of Sesamia nonagrioides[J].Journal of Chemical Ecology,1997,23(11):2437-2444.
[22]Renou M,Nagnan P,Berthier A,et al.Identification of compounds from the eggs of Ostrinia nubilalis,and Mamestra brassicae,having kairomone activity on Trichogramma brassicae[J].Entomologia Experimentalis et Applicata,1992,63(3):291-303.
[23]白树雄,王振营,何康来,等.玉米螟赤眼蜂对亚洲玉米螟益它素的嗅觉反应[J].昆虫学报,2004,47(1):48-54.
[24]Lewis W J,Nordlund D A,Gueldner R C,et al.Kairomones and their use for management of entomophagous insects:XIII.Kairomonal activity for Trichogramma spp.of abdominal tips,excretion,and a synthetic sex pheromone blend of Heliothis zea(Boddie)moths[J].Journal of Chemical Ecology,1981,3(6):513-518.
[25]Arakaki N,Wakamura S,Yasuda T.Phoretic egg parasitoid,Telenomus euproctidis(Hymenoptera:Scelionidae),uses sex pheromone of tussock moth Euproctis taiwana(Lepidoptera:Lymantriidae)as a kairomone[J].Journal of Chemical Ecology,1996,22(6):1079-1085.