纯钛超声微弧氧化-化学镀铜涂层的制备及抗菌性
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摘要
为了赋予纯钛口腔种植体表面涂层生物活性及抗菌性,发挥酸蚀、微弧氧化技术、碱处理及化学镀铜技术各自优势,在TA2纯钛表面制备载铜生物涂层。研究了镀铜时间和温度对载铜含量及涂层表面形貌、结合力和抗菌性的影响。结果表明:钛基体酸蚀获得表面腐蚀均匀的微纳米结构,为超声微弧氧化提供了更多的氧化物生长空间;钛超声微弧氧化涂层碱处理使陶瓷层Ti O2活化,并利于载铜;利用多孔涂层进行化学镀铜时,涂层中铜元素含量随化学镀铜温度的升高和时间增长而增大,而涂层结合力随着化学镀铜时间增长而下降;50℃下化学镀铜3 min时,涂层抗菌率为98.3%,表面铜含量适宜,载铜破坏生物膜的形成使细菌黏附的微环境发生改变,造成细菌难以正常生长,实现了抗菌效果。
Copper- loaded biological coatings were prepared on the surface of TA2 pure titanium.Subsequently,the influences of copper plating time and temperature on the copper content,surface morphology,adhesion strength and antimicrobial properties of the coating were studied.Results showed that Ti substrate obtained a uniform surface with micro-nano corrosion structure by acid- etching,providing more space for the oxide growth during ultrasound micro- arc oxidation.After ultrasonic micro- arc oxidation and coating alkali treatment,the ceramic layer TiO_2 was activated,which was advantageous for loading copper,and then the porous coatings were treated by chemical copper plating.The copper content of the coating was relevant to the plating temperature and processing time,but the adhesion of the coating declined.When copper plating was executed for 3 min at lower than 50℃,the anti-bacterial efficiency of coating was 98.3%,and the copper content on the surface was appropriate.The copper loading on coating destroyed biofilm formation,and changed the micro- environment of bacterial adherent,which inhibited normal growth of the bacteria and accomplished antibacterial effects.
Copper- loaded biological coatings were prepared on the surface of TA2 pure titanium.Subsequently,the influences of copper plating time and temperature on the copper content,surface morphology,adhesion strength and antimicrobial properties of the coating were studied.Results showed that Ti substrate obtained a uniform surface with micro-nano corrosion structure by acid- etching,providing more space for the oxide growth during ultrasound micro- arc oxidation.After ultrasonic micro- arc oxidation and coating alkali treatment,the ceramic layer TiO_2 was activated,which was advantageous for loading copper,and then the porous coatings were treated by chemical copper plating.The copper content of the coating was relevant to the plating temperature and processing time,but the adhesion of the coating declined.When copper plating was executed for 3 min at lower than 50℃,the anti-bacterial efficiency of coating was 98.3%,and the copper content on the surface was appropriate.The copper loading on coating destroyed biofilm formation,and changed the micro- environment of bacterial adherent,which inhibited normal growth of the bacteria and accomplished antibacterial effects.
引文
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[2]Darouiche R O.Treatment of infections associated with surgical implants[J].N Engl J Med,2004,350:1 422~1 429.
[3]冯玉霞,邱宜农.钛种植体表面载银抗菌涂层研究进展[J].中国实用口腔科杂志,2015,8(12):747~751.
[4]巴亚玛,张程程,张卓然,等.载银二氧化钛纳米管涂层种植体对周围软组织炎症反应影响的研究[J].实用口腔医学杂志,2014,30(3):392~396.
[5]Kwok S C H,Zhang W,Wan G J,et al.Hemocompatibility and Anti Bacterial Properties of Silver-Doped Diamond-Like Carbon Prepared by Pulsed Filtered Cathodic Vacuum Arc Deposition[J].Diamond Relat Mater,2007,16:1 353~1 360.
[6]Nyan M,Tsutsumi Y,Oya K,et al.Synthesis of novel oxidelayers on titanium by combination of sputter deposition and micro-arc oxidation techniques[J].Dent Mater J,2011,30(5):754~761.
[7]Abbasi S,Golestani-Fard F,Mirhosseini S M,et al.Effect of electrolyte concentration on microstructure and properties of micro arc oxidized hydroxyapatite/titania nanostructured composite[J].Mater Sci Eng C,2013,33(5):2 555~2 561.
[8]Fernandes J A,Migowski P,Fabrim Z,et al.Ti O2nanotubes sensitized with Cd Se via RF magnetron sputtering for photoelectrochemical applications under visible light irradiation[J].Phys Chem Chem Phys,2014,16(19):9 148~9 153.
[9]Lin D J,Tsai M T,Shieh T M,et al.In vitro antibacterial activity and cytocompatibility of bismuth doped micro-arc oxidized titanium[J].J Biomater Appl,2013,27(5):553~563.
[10]Zhang E L,Li F B,Wang H Y,et al.A new antibacterial titanium-copper sintered alloy:Preparation and antibacterial property[J].Materials Science and Engineering C,2013,33(7):4 280~4 287.
[11]曲立杰,李慕勤,刘苗,等.超声-微弧氧化医用镁合金体外降解性研究[J].稀有金属材料工程,2014,43(1):96~100.
[12]宋应亮,徐君伍,马轩祥,等.口腔环境中纯钛及钛75合金种植体覆盖螺丝缝隙腐蚀的研究[J].口腔医学,2000,20(3):113~115.
[13]Hetrick E M,Schoenfisch M H.Reducing implant-related infections:active release strategies[J].Chem Soc Rev,2006,35(9):780~789.
[14]Dunne W M.Bacterial adhesion:seen any good biofilms lately?[J].Clin Microbiol Rev,2002,15(2):155~166.
[15]Harris L G,Richards R G.Staphylococci and implant surfaces:a review[J].Injury,2006,37(2):S3~S14.
[16]张书源.含铜不锈钢铜离子释放机制研究及其生物学效应探索[D].鞍山:辽宁科技大学,2014:50~53.