盐条件下不同植物生长调节剂对冰菜种子萌发的影响
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摘要
冰菜是一种具有极强耐盐性的新型蔬菜,具有极高的营养价值和保健作用。本文针对冰菜种子萌发率低的问题,采用不同浓度的赤霉素(GA_3)、水杨酸(SA)和抗坏血酸(Vc)等3种植物生长调节剂在盐条件下处理冰菜种子。研究结果表明:冰菜种子萌发的最适盐浓度为10 g·L~(-1)。在此盐度下,若添加10 mg·L~(-1)的GA_3,冰菜种子的发芽率最高可达77.3%;也可添加20 mg·L~(-1)的水杨酸或20 mg·L~(-1)的抗坏血酸,都会对冰菜种子萌发具有明显的促进效果。
This article aims at the question of Mesembryanthemum crystallinum low germination rate,its seed germination was studied by using different concentration exogenous GA_3,different SA ang different Vc with salt. The optimum germination conditions of plant growth regulators were analyzed through a single-factor test. The results indicated that the Mesembryanthemum crystallinum seed germination percentage higher than control at 10 mg·L~(-1) exogenous GA_3, 20 mg·L~(-1) SA and Vc,also the best seed germination percentage under 10 mg·L~(-1) exogenous GA_3 and best index under 20 mg·L~(-1) Vc,which was 77.3% and 16.97%.The study found that three plant growth regulators could improve their germination percentage and index. The results provide a basis theoretical for the large-scale production of Mesembryanthemum crystallinum.
This article aims at the question of Mesembryanthemum crystallinum low germination rate,its seed germination was studied by using different concentration exogenous GA_3,different SA ang different Vc with salt. The optimum germination conditions of plant growth regulators were analyzed through a single-factor test. The results indicated that the Mesembryanthemum crystallinum seed germination percentage higher than control at 10 mg·L~(-1) exogenous GA_3, 20 mg·L~(-1) SA and Vc,also the best seed germination percentage under 10 mg·L~(-1) exogenous GA_3 and best index under 20 mg·L~(-1) Vc,which was 77.3% and 16.97%.The study found that three plant growth regulators could improve their germination percentage and index. The results provide a basis theoretical for the large-scale production of Mesembryanthemum crystallinum.
引文
[1] 陈宏毅.冰菜的生物学特性与栽培技术[J].蔬菜,2016(8):42-45.
[2] 李广鲁,胡增辉,冷平生.冰叶日中花对NaCl胁迫的生理响应[J].北京农学院学报,2015, 30(1):64-70.
[3] 洪磊,刘孟霞.冰菜特征特性及控盐高产栽培技术[J].陕西农业科学, 2015, 61(3):122-124.
[4] 邹焱,苏以荣.植物生长调节剂对烟草生长及营养代谢的调节作用[J].中国土壤与肥料,2006(5):10-14.
[5] 黄玉梅,张杨雪,刘庆林,等.水杨酸对盐胁迫下百日草种子萌发及幼苗生理特性的影响[J].草业学报,2015,24(7):97-105.
[6] 田长恩.植物生长调节剂在烟草上的应用[J].湖北农业科学,1995(3):45-48.
[7] 王芳,庞自学,王汉宁,等.水杨酸浸种对甜玉米种子萌发及生理特性的影响[J].玉米科学,2012, 20(4):74-77.
[8] 徐新娟.赤霉素与种子活力关系研究进展[J].安徽农业科学,2006,34(17):4231-4232.
[9] LU Jinglin, GAO Yanjuan, CHANG Yujiao. Effects of Exogenous Vc on Seed Germination and Physiological Properties of Oil Sunflower(Helianthus annuus) under Salt Stress[J].Agricultural Science & Technology, 2015, 16(3): 500-503.
[10 ] 国际种子检验学会(ISTA).国际种子检验规程[S].北京:技术标准出版社,1980:145-183.
[11] 王宝山,李明亮,张宝泽,等.盐胁迫下外源脯氨酸和丙二醛对冰叶松叶菊愈伤组织中离子和脯氨酸含量的影响[J].植物生理学报,1993(3):182-184.
[12] WAISEL Y. Biology of halophytes[ M] . New York: Academic Press , 1972.
[13] 乌凤章,刘桂丰,姜静,等.种子萌发调控的分子机理研究进展[J].北方园艺,2008(2):54-58.
[14] WARD J M, SMITH A M, SHAH P K, et al.A new role for the Arabidopsis AP2 transcription factor, LEAFY PETIOLE, in gibberellin-induced germination is revealed by the misexpression of a homologousgene, SOB2/DRNLIKE[J]. Plant Cell ,2006, 18:29-39.
[15] 王玉萍, 董雯, 张鑫,等.水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响[J]. 草业学报, 2012, 21(1):213-219.
[16] 张士功,高吉寅,宋景芝.水杨酸和阿斯匹林对小麦盐害的缓解作用[J].植物生理学报, 1999, 25(2):159-164.
[17] 王芳, 庞自学, 王汉宁,等. 水杨酸浸种对甜玉米种子萌发及生理特性的影响[J]. 玉米科学, 2012, 20(4):74-77.
[18] 沈文飚,徐朗莱,叶茂炳.外源抗坏血酸和过氧化氢对小麦离体叶片衰老的调节[J].植物生理学通讯,1997,33( 5) : 338-340.
[19] OMMER-KNUDSEN J, BACIC A, CLARKE A E. Hydroxyproline-rich plant glycoproteins [J]. Phytochemistry, 1998, 47(4) : 483-497.
[20] GARCIA-MUNIZ N, MARTINEZ-IZQUIERDO J A, PUIGDOMENECH P. Induction of mRNA accumulation corresponding to a gene encoding a cell wall hydroxyproline-rich glycoprotein by fungal elicitors[J]. Plant Molecular Biology,1998,38( 4) : 623-632.
[21] 何丽烂,区炳庆,王艳.抗坏血酸和亚硒酸钠浸种对黄瓜幼苗抗氧化功能的影响[J].河北农业科学,2004( 6) : 56-58.
[22] 李铁华,文仕知,彭险峰,等.维生素溶液对闽楠种子活力的影响[J].中南林业科技大学学报,2010,30( 10) : 51-54.
[2] 李广鲁,胡增辉,冷平生.冰叶日中花对NaCl胁迫的生理响应[J].北京农学院学报,2015, 30(1):64-70.
[3] 洪磊,刘孟霞.冰菜特征特性及控盐高产栽培技术[J].陕西农业科学, 2015, 61(3):122-124.
[4] 邹焱,苏以荣.植物生长调节剂对烟草生长及营养代谢的调节作用[J].中国土壤与肥料,2006(5):10-14.
[5] 黄玉梅,张杨雪,刘庆林,等.水杨酸对盐胁迫下百日草种子萌发及幼苗生理特性的影响[J].草业学报,2015,24(7):97-105.
[6] 田长恩.植物生长调节剂在烟草上的应用[J].湖北农业科学,1995(3):45-48.
[7] 王芳,庞自学,王汉宁,等.水杨酸浸种对甜玉米种子萌发及生理特性的影响[J].玉米科学,2012, 20(4):74-77.
[8] 徐新娟.赤霉素与种子活力关系研究进展[J].安徽农业科学,2006,34(17):4231-4232.
[9] LU Jinglin, GAO Yanjuan, CHANG Yujiao. Effects of Exogenous Vc on Seed Germination and Physiological Properties of Oil Sunflower(Helianthus annuus) under Salt Stress[J].Agricultural Science & Technology, 2015, 16(3): 500-503.
[10 ] 国际种子检验学会(ISTA).国际种子检验规程[S].北京:技术标准出版社,1980:145-183.
[11] 王宝山,李明亮,张宝泽,等.盐胁迫下外源脯氨酸和丙二醛对冰叶松叶菊愈伤组织中离子和脯氨酸含量的影响[J].植物生理学报,1993(3):182-184.
[12] WAISEL Y. Biology of halophytes[ M] . New York: Academic Press , 1972.
[13] 乌凤章,刘桂丰,姜静,等.种子萌发调控的分子机理研究进展[J].北方园艺,2008(2):54-58.
[14] WARD J M, SMITH A M, SHAH P K, et al.A new role for the Arabidopsis AP2 transcription factor, LEAFY PETIOLE, in gibberellin-induced germination is revealed by the misexpression of a homologousgene, SOB2/DRNLIKE[J]. Plant Cell ,2006, 18:29-39.
[15] 王玉萍, 董雯, 张鑫,等.水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响[J]. 草业学报, 2012, 21(1):213-219.
[16] 张士功,高吉寅,宋景芝.水杨酸和阿斯匹林对小麦盐害的缓解作用[J].植物生理学报, 1999, 25(2):159-164.
[17] 王芳, 庞自学, 王汉宁,等. 水杨酸浸种对甜玉米种子萌发及生理特性的影响[J]. 玉米科学, 2012, 20(4):74-77.
[18] 沈文飚,徐朗莱,叶茂炳.外源抗坏血酸和过氧化氢对小麦离体叶片衰老的调节[J].植物生理学通讯,1997,33( 5) : 338-340.
[19] OMMER-KNUDSEN J, BACIC A, CLARKE A E. Hydroxyproline-rich plant glycoproteins [J]. Phytochemistry, 1998, 47(4) : 483-497.
[20] GARCIA-MUNIZ N, MARTINEZ-IZQUIERDO J A, PUIGDOMENECH P. Induction of mRNA accumulation corresponding to a gene encoding a cell wall hydroxyproline-rich glycoprotein by fungal elicitors[J]. Plant Molecular Biology,1998,38( 4) : 623-632.
[21] 何丽烂,区炳庆,王艳.抗坏血酸和亚硒酸钠浸种对黄瓜幼苗抗氧化功能的影响[J].河北农业科学,2004( 6) : 56-58.
[22] 李铁华,文仕知,彭险峰,等.维生素溶液对闽楠种子活力的影响[J].中南林业科技大学学报,2010,30( 10) : 51-54.