安融乐对花生田除草剂防效和花生药害的影响
|
摘要
为探讨安融乐对花生田除草剂作用效果的影响,以豫花22为材料,采用安融乐与除草剂混合茎叶喷施,分析加入安融乐后杂草防效、花生药害及叶片生理指标的变化。结果表明,短期内安融乐可提高除草剂对杂草的防效,后期总体上降低精喹禾灵+氟磺胺草醚处理[(45+337.5)、(60+450)g/hm~2)]的除草活性,提高高效氟吡甲禾灵+乙羧氟草醚[(48.6+30)、(64.8+45)g/hm~2]高剂量处理和甲咪唑烟酸72、108 g/hm~2处理的除草活性。精喹禾灵+氟磺胺草醚处理造成花生2~4级药害,过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、低剂量下过氧化氢酶(CAT)活性及高剂量下可溶性糖含量显著升高,加入安融乐使低剂量下药害降低1~2级,对高剂量下药害无明显缓解,POD、APX活性及可溶性糖含量保持显著升高,CAT活性变化表现为低剂量下降至空白对照水平,高剂量下显著升高。高效氟吡甲禾灵+乙羧氟草醚处理初有轻微药害,7 d后症状消失,甲咪唑烟酸处理14 d产生轻微药害,均表现为仅高剂量下APX活性显著升高,加入安融乐,前者处理低剂量下POD、CAT、APX活性显著升高,高剂量下APX活性降至空白对照水平,后者处理高剂量下APX活性仍保持显著升高状态,对其余指标影响较小。因此推测,花生叶片POD、CAT、APX活性及可溶性糖含量的变化可能是安融乐调节花生应对除草剂胁迫的生理反应。
In order to study the effect of Anrongle on herbicidal efficacy in peanut field,the peanut Yuhua22 was taken as material,the foliar spray of Anrongle and herbicide mixture was adopted in fields,and herbicidal efficacy,peanut injury and physiological parameters of injuried leaves were investigated and determined. Results showed that the weed control effect was enhanced by Anrongle in the short term. In the later term,the weed control effects of haloxyfop-P-methyl + fluoroglycofen-ethyl( 64. 8 + 45) g/ha and imazapic 72,108 g/ha were enhanced,while those were obviously reduced for quizalofop-P-ethyl + fomesafen to control weeds by Anrongle. A 2—4 level injury and an obvious enhancement in activities of peroxidase( POD),ascorbic acid peroxidase( APX),and catalase( CAT) under lower dose,and in content of soluble sugar under higher dose were presented when treated with quizalofop-P-ethyl + fomesafen. When it was applied with Anrongle,the injury decreased by 1—2 level at lower dose,and no obvious alleviation was investigated at higher dose,the activities of POD,APX and the content of soluble sugar were still significantly enhanced,the CAT activity was dropped to CK level at lower dose and significantly enhanced athigher dose. When treated with haloxyfop-P-methyl + fluoroglycofen-ethyl and imazapic,slight injury was presented in a short-term and 14 days after treating,respectively,the former injury disappeared in 7 days,both the APX activity at higher dose showed a significant enhancement. When Anrongle was added,POD,CAT and APX activities in the former at lower dose were significantly enhanced,APX activity at higher dose was dropped to CK level,in the latter APX activity at higher dose still kept significant enhancement,while other indexes were affected less. Therefore,the change of CAT,APX,POD activities and soluble sugar content might be the physiological reaction for Anrongle adjusting peanut growth under herbicidal stress.
In order to study the effect of Anrongle on herbicidal efficacy in peanut field,the peanut Yuhua22 was taken as material,the foliar spray of Anrongle and herbicide mixture was adopted in fields,and herbicidal efficacy,peanut injury and physiological parameters of injuried leaves were investigated and determined. Results showed that the weed control effect was enhanced by Anrongle in the short term. In the later term,the weed control effects of haloxyfop-P-methyl + fluoroglycofen-ethyl( 64. 8 + 45) g/ha and imazapic 72,108 g/ha were enhanced,while those were obviously reduced for quizalofop-P-ethyl + fomesafen to control weeds by Anrongle. A 2—4 level injury and an obvious enhancement in activities of peroxidase( POD),ascorbic acid peroxidase( APX),and catalase( CAT) under lower dose,and in content of soluble sugar under higher dose were presented when treated with quizalofop-P-ethyl + fomesafen. When it was applied with Anrongle,the injury decreased by 1—2 level at lower dose,and no obvious alleviation was investigated at higher dose,the activities of POD,APX and the content of soluble sugar were still significantly enhanced,the CAT activity was dropped to CK level at lower dose and significantly enhanced athigher dose. When treated with haloxyfop-P-methyl + fluoroglycofen-ethyl and imazapic,slight injury was presented in a short-term and 14 days after treating,respectively,the former injury disappeared in 7 days,both the APX activity at higher dose showed a significant enhancement. When Anrongle was added,POD,CAT and APX activities in the former at lower dose were significantly enhanced,APX activity at higher dose was dropped to CK level,in the latter APX activity at higher dose still kept significant enhancement,while other indexes were affected less. Therefore,the change of CAT,APX,POD activities and soluble sugar content might be the physiological reaction for Anrongle adjusting peanut growth under herbicidal stress.
引文
[1]马旭俊,朱大海.植物超氧化物歧化酶(SOD)的研究进展[J].遗传,2003,25(2):225-231.
[2]王群,尹飞,李潮海.水分胁迫下植物体内活性氧自由基代谢研究进展[J].河南农业科学,2004(10):25-28.
[3]原向阳,郭平毅,张丽光,等.干旱胁迫下草甘膦对抗草甘膦大豆幼苗保护酶活性及脂质过氧化作用的影响[J].中国农业科学,2010,43(4):698-705.
[4]Xu Q,Huang B R.Antioxidant metabolism associated with summer leaf senescence and turf quality decline for creeping bentgrass[J].Crop Science,2004,44(2):553-560.
[5]Weismann D,Hartvigsen K,Lauer N,et al.Complement factor H binds malondialdehyde epitopes and protects from oxidative stress[J].Nature,2011,478(7367):76-81.
[6]张燕,陈静,田军熊,等.低磷胁迫对玉米抗氧化系统及可溶性糖含量阶段性变化的影响[J].黑龙江农业科学,2015(11):11-16.
[7]刘亦学,刘焕禄,张学文,等.除草剂药害及其预防和补救[J].天津农林科技,2005(6):18-20.
[8]王险峰,范志伟,胡荣娟,等.除草剂药害新进展与解决方法[J].农药,2009,48(5):384-387.
[9]黄允才,张格成,胡光华.天然芸苔素缓解除草剂药害的作用[J].农药,2000,39(6):41-42.
[10]Xinke T,Xiaomao Z,Jing W,et al.A novel function of sanshools:The alleviation of injury from metolachlor in rice seedlings[J].Pesticide Biochemistry and Physiology,2014,110(1):44-49.
[11]马菁平,朱昌华,夏凯,等.卵磷脂和氯化胆碱对玉米幼苗生长及抗冷性的影响[J].江苏农业科学,2008(5):24-27.
[12]陈德西,何忠全,刘欢,等.植物源调节剂碧护组合技术对提升茶叶产量的效果研究[J].资源开发与市场,2017,33(3):360-363.
[13]王亮亮,韩效钊,杨静芳,等.生物表面活性剂对水溶肥料润湿性能的影响[J].浙江农业科学,2014(8):1253-1255.
[14]杜慧平,张治家.山西大白菜花叶病毒病防控技术[J].山西农业科学,2017,45(7):1149-1152.
[15]陶惠.不同化学药剂组合防治马铃薯晚疫病药效示范[J].现代农业科技,2017(8):105-106.
[16]陈建勋,王晓峰.植物生理学实验指导[M].2版.广州:华南理工大学出版社,2006:1-100.
[17]刘学,顾宝根.农药生物活性测试标准操作规范:除草剂卷[M].北京:化学工业出版社,2016:198-200.
[18]陈新华,郭宝林,赵静,等.休眠期内甜樱桃不同品种枝条的抗寒性[J].河北农业大学学报,2009,32(6):37-40.
[19]赵超,王海燕,刘美珍,等.干旱胁迫下木薯茎秆可溶性糖、淀粉及相关酶的代谢规律[J].植物生理学报,2017,53(5):795-806.
[20]赵曾菁,宋奇琦,赵虎,等.模拟霜冻条件对黄瓜幼苗生长及抗氧化酶活性的影响[J].南方农业学报,2018,49(4):757-762.
[21]张庆琛,郑少萌,刘应敏,等.干旱胁迫对冬小麦幼苗叶片光合生理特性的影响[J].山西农业科学,2016,44(8):1077-1082.
[2]王群,尹飞,李潮海.水分胁迫下植物体内活性氧自由基代谢研究进展[J].河南农业科学,2004(10):25-28.
[3]原向阳,郭平毅,张丽光,等.干旱胁迫下草甘膦对抗草甘膦大豆幼苗保护酶活性及脂质过氧化作用的影响[J].中国农业科学,2010,43(4):698-705.
[4]Xu Q,Huang B R.Antioxidant metabolism associated with summer leaf senescence and turf quality decline for creeping bentgrass[J].Crop Science,2004,44(2):553-560.
[5]Weismann D,Hartvigsen K,Lauer N,et al.Complement factor H binds malondialdehyde epitopes and protects from oxidative stress[J].Nature,2011,478(7367):76-81.
[6]张燕,陈静,田军熊,等.低磷胁迫对玉米抗氧化系统及可溶性糖含量阶段性变化的影响[J].黑龙江农业科学,2015(11):11-16.
[7]刘亦学,刘焕禄,张学文,等.除草剂药害及其预防和补救[J].天津农林科技,2005(6):18-20.
[8]王险峰,范志伟,胡荣娟,等.除草剂药害新进展与解决方法[J].农药,2009,48(5):384-387.
[9]黄允才,张格成,胡光华.天然芸苔素缓解除草剂药害的作用[J].农药,2000,39(6):41-42.
[10]Xinke T,Xiaomao Z,Jing W,et al.A novel function of sanshools:The alleviation of injury from metolachlor in rice seedlings[J].Pesticide Biochemistry and Physiology,2014,110(1):44-49.
[11]马菁平,朱昌华,夏凯,等.卵磷脂和氯化胆碱对玉米幼苗生长及抗冷性的影响[J].江苏农业科学,2008(5):24-27.
[12]陈德西,何忠全,刘欢,等.植物源调节剂碧护组合技术对提升茶叶产量的效果研究[J].资源开发与市场,2017,33(3):360-363.
[13]王亮亮,韩效钊,杨静芳,等.生物表面活性剂对水溶肥料润湿性能的影响[J].浙江农业科学,2014(8):1253-1255.
[14]杜慧平,张治家.山西大白菜花叶病毒病防控技术[J].山西农业科学,2017,45(7):1149-1152.
[15]陶惠.不同化学药剂组合防治马铃薯晚疫病药效示范[J].现代农业科技,2017(8):105-106.
[16]陈建勋,王晓峰.植物生理学实验指导[M].2版.广州:华南理工大学出版社,2006:1-100.
[17]刘学,顾宝根.农药生物活性测试标准操作规范:除草剂卷[M].北京:化学工业出版社,2016:198-200.
[18]陈新华,郭宝林,赵静,等.休眠期内甜樱桃不同品种枝条的抗寒性[J].河北农业大学学报,2009,32(6):37-40.
[19]赵超,王海燕,刘美珍,等.干旱胁迫下木薯茎秆可溶性糖、淀粉及相关酶的代谢规律[J].植物生理学报,2017,53(5):795-806.
[20]赵曾菁,宋奇琦,赵虎,等.模拟霜冻条件对黄瓜幼苗生长及抗氧化酶活性的影响[J].南方农业学报,2018,49(4):757-762.
[21]张庆琛,郑少萌,刘应敏,等.干旱胁迫对冬小麦幼苗叶片光合生理特性的影响[J].山西农业科学,2016,44(8):1077-1082.