非转基因抗除草剂玉米对三种乙酰乳酸合成酶抑制剂类除草剂的田间抗性表现
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摘要
为评估中国农业大学培育的非转基因抗除草剂玉米品系958R和335R在大田条件下对乙酰乳酸合成酶(acetolactate synthase,ALS)抑制剂类除草剂的抗性表现,利用甲咪唑烟酸、砜嘧磺隆、唑嘧磺草胺3种除草剂对郑单958、958R、先玉335、335R共4种玉米杂交种进行了播后苗前土壤处理,每种除草剂设3个处理剂量(1、3、9倍推荐剂量),并于施药2周和4周后进行株高测定,于收获晾干后测产。结果表明,在甲咪唑烟酸216、648 g (a.i.)/hm~2处理下,郑单958和先玉335均已绝产,而958R和335R产量均未受影响;在砜嘧磺隆或唑嘧磺草胺高剂量处理下,常规玉米品种郑单958和先玉335株高的最高降幅分别为25.7%和35.2%,田间药害反应显著,而958R和335R则抗性反应显著。研究表明,非转基因抗除草剂玉米杂交种具有良好的田间抗性,不仅能有效解决玉米田砜嘧磺隆和唑嘧磺草胺等ALS除草剂的药害问题,还能够通过引入甲咪唑烟酸等新的ALS除草剂更好地防除玉米田的杂草。
The objective of this study is to investigate whether these non-transgenic imidazolinone resistant(IR) maize hybrids could show desired tolerance to various herbicide treatments under field condition in Beijing, China. Imazapic, rimsulfuron, and flumetsulam at one time, three times, nine times of the respective field recommendation rates were soil-applied to plots planted with IR maize hybrids,958R and 335R, and conventional hybrids, Z958 and X335. Plant heights were measured at two and four weeks after treatment, and yields were recorded after harvest. The results showed that conventional hybrids produced no yield when treated with imazapic at 216 g(a.i.)/hm~2 and 648 g(a.i.)/hm~2, while the yields of IR maize hybrids were not affected. For rimsulfuron and flumetsulam, which were registered for maize, caused significant crop damage, up to 25.7% and 35.2% plant height reduction for Z958 and X335, at the highest application rates respectively; however, IR maize hybrids were more tolerant to rimsulfuron and flumetsulam than conventional hybrids. The results indicated that IR maize hybrids showed high level of herbicide resistance, thus providing a great tool not only to address current crop injury caused by maize-registered ALS herbicides including rimsulfuron and flumetsulam, but also to in-troduce new ALS herbicides to control weed in maize field in China.
The objective of this study is to investigate whether these non-transgenic imidazolinone resistant(IR) maize hybrids could show desired tolerance to various herbicide treatments under field condition in Beijing, China. Imazapic, rimsulfuron, and flumetsulam at one time, three times, nine times of the respective field recommendation rates were soil-applied to plots planted with IR maize hybrids,958R and 335R, and conventional hybrids, Z958 and X335. Plant heights were measured at two and four weeks after treatment, and yields were recorded after harvest. The results showed that conventional hybrids produced no yield when treated with imazapic at 216 g(a.i.)/hm~2 and 648 g(a.i.)/hm~2, while the yields of IR maize hybrids were not affected. For rimsulfuron and flumetsulam, which were registered for maize, caused significant crop damage, up to 25.7% and 35.2% plant height reduction for Z958 and X335, at the highest application rates respectively; however, IR maize hybrids were more tolerant to rimsulfuron and flumetsulam than conventional hybrids. The results indicated that IR maize hybrids showed high level of herbicide resistance, thus providing a great tool not only to address current crop injury caused by maize-registered ALS herbicides including rimsulfuron and flumetsulam, but also to in-troduce new ALS herbicides to control weed in maize field in China.
引文
Chen YY,Wang ZP,Ni HW,Xu Y,Chen QJ,Jiang LJ.2017.CRISPR/Cas9-mediated base-editing system efficiently generates gain-offunction mutations in Arabidopsis.Science China Life Sciences,60(5):520-523
Fang J,Piao ZZ,Wan CZ,Bai JJ,Yang RF.2017.Maize imidazolinoneresistant protein,sequence and its application:CN106834260A.2017-06-13(in Chinese)[方军,朴钟泽,万常照,白建江,杨瑞芳.2017.具有抗咪唑啉酮类除草剂活性的玉米蛋白质、其编码基因及应用:CN106834260A.2017-06-13]
Gaines TA,Zhang W,Wang D,Bukun B,Chisholm ST,Shaner DL,Nissen SJ,Patzoldt WL,Tranel PJ,Culpepper AS,et al.2010.Gene amplification confers glyphosate resistance in Amaranthus palmeri.Proceedings of the National Academy of Sciences of the United States of America,107(3):1029-1034
Goloubinoff P,Edelman M,Hallick RB.1984.Chloroplast-coded atrazine resistance in Solanum nigrum:psbA loci from susceptible and resistant biotypes are isogenic except for a single codon change.Nucleic Acids Research,12(24):9489-9496
Hall MR,Swanton CJ,Anderson GW.1992.The critical period of weed control in grain corn(Zea mays).Weed Science,40(3):441-447
Ma LG,Wang Z,Huang BY,Shi JY,Deng XW.2014.Maize herbicide resistant protein and its application:CN103103176B.2014-03-26(in Chinese)[马力耕,王峥,黄拔严,石久英,邓兴旺.2014.玉米抗除草剂蛋白及其在植物育种中的应用:CN103103176B.2014-03-26]
O’Sullivan J,Bouw WJ.1998.Sensitivity of processing sweet corn(Zea mays)cultivars to nicosulfuron/rimsulfuron.Canadian Journal of Plant Science,78(1):151-154
Ransom J,Kanampiu F,Gressel J,Groote HD,Burnet M,Odhiambo G.2012.Herbicide applied to imidazolinone resistant-maize seed as a Striga control option for small-scale African farmers.Weed Science,60(2):283-289
Ritter RL,Menbere H.2001.Preemergence and postemergence control of triazine-resistant common lambsquarters(Chenopodium album)in no-till corn(Zea mays).Weed Technology,15(4):879-884
Stall WM,Bewick TA.1992.Sweet corn cultivars respond differentially to the herbicide nicosulfuron.HortScience,27(2):131-133
Tan SY,Evans RR,Dahmer ML,Singh BK,Shaner DL.2005.Imidazolinone-tolerant crops:history,current status and future.Pest Management Science,61(3):246-257
Tao B,Zhang HY,Zhang QH,Han YJ.2010.Current knowledge and future challenges of non-genetically modified herbicide-resistant crops.Journal of Plant Protection,37(3):277-282(in Chinese)[陶波,张洪岩,张庆贺,韩玉军.2010.非转基因抗除草剂作物研究现状与展望.植物保护学报,37(3):277-282]
The International Service for the Acquisition of Agri-biotech Applications.2016.Global status of commercialized biotech/gm crops:2016.ISAAA Brief No.52.ISAAA:Ithaca,NY
Thompson CJ,Movva NR,Tizard R,Crameri R,Davies JE,Lauwereys M,Botterman J.1987.Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus.The EMBOJournal,6(9):2519-2523
Wang J,Zhong XM,LüXL,Li FH,Shi ZS.2016.Advances in research on tolerance of differential corn cultivars to the nicosulfuron.Chinese Journal of Pesticide Science,18(3):282-290(in Chinese)[王健,钟雪梅,吕香玲,李凤海,史振声.2016.不同品种玉米对烟嘧磺隆的耐药性研究进展.农药学学报,18(3):282-290]
Wang XF,Fan ZW,Hu RJ,Zou W,Tong YC.2009.Progress and solution of herbicide phytotoxicity.Agrochemicals,48(5):384-388(in Chinese)[王险峰,范志伟,胡荣娟,邹伟,仝育存.2009.除草剂药害新进展与解决方法.农药,48(5):384-388]
Yu Q,Powles SB.2014.Resistance to AHAS inhibitor herbicides:current understanding.Pest Management Science,70(9):1340-1350
Zhang ZF,Gao SC.2012.Control of velvetleaf by flumetsulam in corn field.Modernizing Agriculture,(2):4(in Chinese)[张子丰,高士才.2012.阔草清防除玉米田苘麻示范试验.现代化农业,(2):4]
Zhou ZL.2009.Causes of herbicide damage in maize field and preventive measures.Agricultural Science and Technology and Equipment,(5):17-18,20(in Chinese)[周振龙.2009.玉米田除草剂药害发生原因及防治措施.农业科技与装备,(5):17-18,20]
Zuver KA,Bernards ML,Kells JJ,Sprague CL,Medlin CR,Loux MM.2006.Evaluation of postemergence weed control strategies in herbicide-resistant isolines of corn(Zea mays).Weed Technology,20(1):172-178
Fang J,Piao ZZ,Wan CZ,Bai JJ,Yang RF.2017.Maize imidazolinoneresistant protein,sequence and its application:CN106834260A.2017-06-13(in Chinese)[方军,朴钟泽,万常照,白建江,杨瑞芳.2017.具有抗咪唑啉酮类除草剂活性的玉米蛋白质、其编码基因及应用:CN106834260A.2017-06-13]
Gaines TA,Zhang W,Wang D,Bukun B,Chisholm ST,Shaner DL,Nissen SJ,Patzoldt WL,Tranel PJ,Culpepper AS,et al.2010.Gene amplification confers glyphosate resistance in Amaranthus palmeri.Proceedings of the National Academy of Sciences of the United States of America,107(3):1029-1034
Goloubinoff P,Edelman M,Hallick RB.1984.Chloroplast-coded atrazine resistance in Solanum nigrum:psbA loci from susceptible and resistant biotypes are isogenic except for a single codon change.Nucleic Acids Research,12(24):9489-9496
Hall MR,Swanton CJ,Anderson GW.1992.The critical period of weed control in grain corn(Zea mays).Weed Science,40(3):441-447
Ma LG,Wang Z,Huang BY,Shi JY,Deng XW.2014.Maize herbicide resistant protein and its application:CN103103176B.2014-03-26(in Chinese)[马力耕,王峥,黄拔严,石久英,邓兴旺.2014.玉米抗除草剂蛋白及其在植物育种中的应用:CN103103176B.2014-03-26]
O’Sullivan J,Bouw WJ.1998.Sensitivity of processing sweet corn(Zea mays)cultivars to nicosulfuron/rimsulfuron.Canadian Journal of Plant Science,78(1):151-154
Ransom J,Kanampiu F,Gressel J,Groote HD,Burnet M,Odhiambo G.2012.Herbicide applied to imidazolinone resistant-maize seed as a Striga control option for small-scale African farmers.Weed Science,60(2):283-289
Ritter RL,Menbere H.2001.Preemergence and postemergence control of triazine-resistant common lambsquarters(Chenopodium album)in no-till corn(Zea mays).Weed Technology,15(4):879-884
Stall WM,Bewick TA.1992.Sweet corn cultivars respond differentially to the herbicide nicosulfuron.HortScience,27(2):131-133
Tan SY,Evans RR,Dahmer ML,Singh BK,Shaner DL.2005.Imidazolinone-tolerant crops:history,current status and future.Pest Management Science,61(3):246-257
Tao B,Zhang HY,Zhang QH,Han YJ.2010.Current knowledge and future challenges of non-genetically modified herbicide-resistant crops.Journal of Plant Protection,37(3):277-282(in Chinese)[陶波,张洪岩,张庆贺,韩玉军.2010.非转基因抗除草剂作物研究现状与展望.植物保护学报,37(3):277-282]
The International Service for the Acquisition of Agri-biotech Applications.2016.Global status of commercialized biotech/gm crops:2016.ISAAA Brief No.52.ISAAA:Ithaca,NY
Thompson CJ,Movva NR,Tizard R,Crameri R,Davies JE,Lauwereys M,Botterman J.1987.Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus.The EMBOJournal,6(9):2519-2523
Wang J,Zhong XM,LüXL,Li FH,Shi ZS.2016.Advances in research on tolerance of differential corn cultivars to the nicosulfuron.Chinese Journal of Pesticide Science,18(3):282-290(in Chinese)[王健,钟雪梅,吕香玲,李凤海,史振声.2016.不同品种玉米对烟嘧磺隆的耐药性研究进展.农药学学报,18(3):282-290]
Wang XF,Fan ZW,Hu RJ,Zou W,Tong YC.2009.Progress and solution of herbicide phytotoxicity.Agrochemicals,48(5):384-388(in Chinese)[王险峰,范志伟,胡荣娟,邹伟,仝育存.2009.除草剂药害新进展与解决方法.农药,48(5):384-388]
Yu Q,Powles SB.2014.Resistance to AHAS inhibitor herbicides:current understanding.Pest Management Science,70(9):1340-1350
Zhang ZF,Gao SC.2012.Control of velvetleaf by flumetsulam in corn field.Modernizing Agriculture,(2):4(in Chinese)[张子丰,高士才.2012.阔草清防除玉米田苘麻示范试验.现代化农业,(2):4]
Zhou ZL.2009.Causes of herbicide damage in maize field and preventive measures.Agricultural Science and Technology and Equipment,(5):17-18,20(in Chinese)[周振龙.2009.玉米田除草剂药害发生原因及防治措施.农业科技与装备,(5):17-18,20]
Zuver KA,Bernards ML,Kells JJ,Sprague CL,Medlin CR,Loux MM.2006.Evaluation of postemergence weed control strategies in herbicide-resistant isolines of corn(Zea mays).Weed Technology,20(1):172-178