GC-MS分析荷花的挥发油化学成分
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摘要
为了分析荷花中挥发油的化学成分,采用水蒸气蒸馏法从新鲜荷花中提取挥发油,用气相色谱-质谱联用技术(GC-MS)对挥发油的化学成分进行分离分析,并应用峰面积归一化法测定各成分的含量。试验共分离出95个峰,鉴定出80种化合物,其中主要成分为乙酸乙酯(6.61%)、1,4-二甲氧基苯(9.06%)、肉桂醛(6.60%)、肉桂醇(10.20%)、茉莉酮(4.75%)等挥发性的醇类、脂类、醛类、酸类和烯类化合物。通过试验可知,GC-MS能很好地鉴定出荷花挥发油的化合物组成,为荷花资源的开发利用奠定基础。
In order to analyze the chemical constituents of volatile oil in lotus flower, this paper used the method of steam distillation to extract volatile oil from fresh lotus flower. The chemical constituents of volatile oil were separated and analyzed by gas chromatography-mass spectrometry(GC-MS), and the percentage content of each component was determined by peak area normalization method. A total of 95 peaks were isolated and 80 compounds were identified, including acetic ether(6.61%), 1,4-dimethoxy benzene(9.06%), Cinnamic aldehyde(6.60%), Cinnamic alcohol(10.20%), Jasmone(4.75%) and other volatile alcohols, lipids, aldehydes, acids and olefins. The results showed that GC-MS can determine the composition of volatile oil of lotus well and lay a foundation for the development and utilization of lotus resources.
In order to analyze the chemical constituents of volatile oil in lotus flower, this paper used the method of steam distillation to extract volatile oil from fresh lotus flower. The chemical constituents of volatile oil were separated and analyzed by gas chromatography-mass spectrometry(GC-MS), and the percentage content of each component was determined by peak area normalization method. A total of 95 peaks were isolated and 80 compounds were identified, including acetic ether(6.61%), 1,4-dimethoxy benzene(9.06%), Cinnamic aldehyde(6.60%), Cinnamic alcohol(10.20%), Jasmone(4.75%) and other volatile alcohols, lipids, aldehydes, acids and olefins. The results showed that GC-MS can determine the composition of volatile oil of lotus well and lay a foundation for the development and utilization of lotus resources.
引文
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[10]朱贤森,黄晓东,黄志勤.肉桂醛抗白假丝酵母菌实验研究[J].时珍国医国药,2009,20(9):2302-2303.
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[2]王其超,张行言.浅析《本草纲目》与荷花[J].中国园林2006,22(03):79-81.
[3]GUO HB.Cultivation of Lotus(Nelumbo nucifera Gaertn.ssp nucifera)and its Utilization in China[J].Genetic Resources and Crop Evolution,2009,56(3):323-330.
[4]林宣贤.荷花的营养成分和安全性研究[J].食品科学2006,27(10):538-539.
[5]YANG RZ,WEI XL,GAO FF,et al.Simultaneous analysis of anthocyanins and flavonols in petals of lotus(Nelumbo)cultivars by high-performance liquid chromatographyphotodiode array detection/electrospray ionization mass spectrometry[J].Journal of Chromatography A,2009,1216(1):106-112.
[6]GUO XF,WANG DJ,DUAN WJ,et al.Preparative isolation and purification of four flavonoids from the petals of nelumbo nucifera by high-speed counter-current chromatography[J].Phytochemical Analysis,2010,21(3):268-272.
[7]鲍忠定,秦志荣,许荣年,等.杭白菊挥发油化学成分的气相色谱-质谱联用技术分析[J].食品科学,2003,24(6):120-121.
[8]孙宝国,何坚.香料化学与工艺学[M].北京:化学工业出版社,2004.
[9]谢小梅,龙凯,许杨,等.肉桂醛和柠檬醛对黄曲霉及烟曲霉细胞DNA与RNA的影响[J].中草药,2005,36(4):558-560.
[10]朱贤森,黄晓东,黄志勤.肉桂醛抗白假丝酵母菌实验研究[J].时珍国医国药,2009,20(9):2302-2303.
[11]乔卫,张衍文.天然环烯醚菇类化合物的生物活性[J].国外医药·植物药分册,2001,(2):65-67.