桃红四物汤调节NF-κB信号通路保护2型糖尿病模型大鼠心脏结构和功能
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摘要
目的:观察桃红四物汤对2型糖尿病(T2DM)模型大鼠心脏结构和功能的干预作用及对模型大鼠心肌组织NF-κB信号通路相关分子表达的影响。方法:采用高糖高脂饲料联合链脲佐菌素制备T2DM模型大鼠,造模成功后,药物连续干预8周,观察各组大鼠心脏血流动力学及心肌组织形态学变化,同时检测大鼠心肌组织NF-κBp65、TNF-α蛋白及mRNA表达和血清IL-1β、IL-6含量。结果:T2DM模型大鼠出现显著心脏结构和功能损伤。与正常组比较,模型组大鼠心肌组织NF-κBp65、TNF-α蛋白和mRNA表达显著增加(P<0.01),血清IL-1β、IL-6含量显著升高(P<0.01)。桃红四物汤低、中、高剂量组干预8周后,可显著改善T2DM模型大鼠心脏结构和功能的损伤。与模型组比较,桃红四物汤低、中、高剂量组大鼠心肌NF-κBp65、TNF-α蛋白和mRNA表达显著减少(P<0.05,P<0.01),血清IL-1β、IL-6含量显著降低(P<0.05,P<0.01)。结论:桃红四物汤可有效改善T2DM模型大鼠心脏结构和功能,其机制与调节NF-κB信号通路过度激活有关。
Objective: To observe Taohong Siwu Decoction's intervention effect on cardiac structure and function of type2 diabetes mellitus(T2 DM) model rats and its effect on molecule expression related to myocardial tissue NF-κB signal pathway.Methods: Rats were feeding with high-fat and high-sugar foods with streptozotocin i.p. Use medicine to intervene the T2 DM models continuously for 8 weeks, then observe rats' cardiac hemodynamics and myocardial histomorphological changes, and detect rats' NF-κBp65, TNF-α proteins and mRNA expression of myocardial tissue, and IL-1β, IL-6 levels in their sera. Results:T2 DM model rats got obvious damages in both cardiac structures and functions. Comparing with the normal group, the expression of NF-κBp65, TNF-α proteins and mRNA of rats of myocardial tissue were significantly increased(P<0.01), IL-1β and IL-6 levels in their serum were significantly increased(P<0.01); After the interventions of Taohong Siwu Decoction in each dosage group for 8 weeks, the model rats' cardiac structures and functions improved significantly, their NF-κBp65, TNF-α proteins and mRNA expression of myocardial tissues were significantly decreased(P<0.05, P<0.01), IL-1β and IL-6 levels in their serum were significantly decreased(P<0.05, P<0.01). Conclusion: Taohong Siwu Decoction can effectively improve the T2 DM model rats' cardiac structure and function, and the mechanism is related to its regulation of over-activated NF-κB signal pathway.
Objective: To observe Taohong Siwu Decoction's intervention effect on cardiac structure and function of type2 diabetes mellitus(T2 DM) model rats and its effect on molecule expression related to myocardial tissue NF-κB signal pathway.Methods: Rats were feeding with high-fat and high-sugar foods with streptozotocin i.p. Use medicine to intervene the T2 DM models continuously for 8 weeks, then observe rats' cardiac hemodynamics and myocardial histomorphological changes, and detect rats' NF-κBp65, TNF-α proteins and mRNA expression of myocardial tissue, and IL-1β, IL-6 levels in their sera. Results:T2 DM model rats got obvious damages in both cardiac structures and functions. Comparing with the normal group, the expression of NF-κBp65, TNF-α proteins and mRNA of rats of myocardial tissue were significantly increased(P<0.01), IL-1β and IL-6 levels in their serum were significantly increased(P<0.01); After the interventions of Taohong Siwu Decoction in each dosage group for 8 weeks, the model rats' cardiac structures and functions improved significantly, their NF-κBp65, TNF-α proteins and mRNA expression of myocardial tissues were significantly decreased(P<0.05, P<0.01), IL-1β and IL-6 levels in their serum were significantly decreased(P<0.05, P<0.01). Conclusion: Taohong Siwu Decoction can effectively improve the T2 DM model rats' cardiac structure and function, and the mechanism is related to its regulation of over-activated NF-κB signal pathway.
引文
[1]冯新星,陈燕燕.糖尿病心肌病的研究进展.中国循环杂志,2015,30(1):87-91
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[8]党涛,王煜,刘丽华,等.吡格列酮和依那普利对糖尿病心肌病大鼠心脏收缩功能的影响.甘肃科学学报,2015,27(1):62-67
[9]任晓英,王战建.2型糖尿病大血管病变与炎症的关系及吡格列酮的保护作用.中国糖尿病杂志,2008,16(8):491-493
[10]胡佳卉,孟庆刚.基于Cite Space的中药治疗2型糖尿病知识图谱分析.中华中医药杂志,2017,32(9):4102-4106
[11]王栋栋,何素梅,张冠英,等.大黄游离蒽醌对糖尿病大鼠心肌纤维化的作用.中国药理学通报,2015,31(4):509-513
[2]潘大彬,汪旻晖,曹蘅.糖尿病心肌病发病机制的研究进展.中国临床药理学与治疗学,2013,18(7):831-836
[3]郑海霞,李明波.桃红四物汤加减在治疗2型糖尿病中的临床观察.中国医药科学,2014,4(24):78-80
[4]连红琴,秦铮然,张月虹,等.桃红四物汤的临床应用及实验研究进展.中华中医药学刊,2010,28(9):1868-1870
[5]董世芬,洪缨,汪瑞祺,等.小檗碱对实验性2型糖尿病心肌病大鼠模型心脏保护作用研究.中国药理学通报,2013,29(9):1216-1221
[6]董世芬,洪缨,孙建宁,等.实验性糖尿病心肌病大鼠模型建立及心脏功能和结构相关性分析.中国实验动物学报,2010,18(6):457-460
[7]梁茜,杨希立,梅百强.吡格列酮对2型糖尿病合并冠心病患者动脉粥样硬化相关因素及其预后的影响.实用医学杂志,2011,27(17):3216-3219
[8]党涛,王煜,刘丽华,等.吡格列酮和依那普利对糖尿病心肌病大鼠心脏收缩功能的影响.甘肃科学学报,2015,27(1):62-67
[9]任晓英,王战建.2型糖尿病大血管病变与炎症的关系及吡格列酮的保护作用.中国糖尿病杂志,2008,16(8):491-493
[10]胡佳卉,孟庆刚.基于Cite Space的中药治疗2型糖尿病知识图谱分析.中华中医药杂志,2017,32(9):4102-4106
[11]王栋栋,何素梅,张冠英,等.大黄游离蒽醌对糖尿病大鼠心肌纤维化的作用.中国药理学通报,2015,31(4):509-513