线粒体动力相关蛋白FIS1调控舌鳞癌顺铂敏感性的机制研究
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摘要
目的探讨线粒体动力相关蛋白(mitochondrial fission protein 1,FIS1)对于舌鳞癌细胞的凋亡以及顺铂耐药性的调控作用。方法使用舌鳞癌细胞系SCC9和CAL27,检测顺铂处理后这两种细胞中FIS1的mRNA以及蛋白质水平,采用小干扰RNA(small interfere RNA,siRNA)沉默经顺铂处理后的SCC9和CAL27细胞中的FIS1,或者利用质粒过表达SCC9和CAL27细胞中的FIS1,之后采用线粒体染色检测细胞中线粒体的分裂状态,TUNEL、流式细胞术、Caspase3/7检测细胞的凋亡状态。结果经顺铂处理后的舌鳞癌细胞中FIS1蛋白表达水平升高,但mRNA水平未发生变化。沉默FIS1表达可减少顺铂处理后的舌鳞癌细胞中的线粒体分裂以及细胞凋亡,而过表达FIS1则可以观察到相反效应。沉默或者过表达FIS1前后,SCC9和CAL27细胞中分裂线粒体所占的百分比,凋亡细胞的含量以及Caspase3/7的活性值差异有统计学意义(P <0.05)。结论FIS1参与调控舌鳞癌细胞顺铂化疗敏感性,可作为提高口腔鳞癌顺铂化疗敏感性的新靶点。
Objective To investigate the effect of mitochondrial fission protein 1(FIS1) on apoptosis and cisplatin resistance in tongue squamous cell carcinoma(TSCC) cells. Methods The squamous cell carcinoma cell lines SCC9 and CAL27 were used to detect the mRNA and protein levels of FIS1 after cisplatin treatment, the knockdown and overexpression of FIS1 of SCC9 and CAL27 with or without cisplatin treatment were accomplished through small interfering RNA(siRNA) and plasmid, respectively. The mitochondrial division state in cells was detected by mitochondrial staining, and the apoptosis state of cells was detected by TUNEL, flow cytometry and Caspase 3/7. Results FIS1 protein expression in tongue squamous carcinoma cells treated with cisplatin was increased, but the mRNA level did not change.Silencing of FIS1 expression reduced mitochondrial division and apoptosis in squamous cell carcinoma cells treated with cisplatin, whereas overexpression of FIS1 exhibited the opposite effects. The percentage of dividing mitochondria,the number of apoptotic cells and the activity of Caspase 3/7 in SCC9 and CAL27 cells were significantly different before and after modulation of FIS1 expression(P < 0.05). Conclusion FIS1 is involved in the regulation of cisplatin chemotherapy sensitivity in tongue squamous cell carcinoma and can be used as a new target for improving the sensitivity of cisplatin chemotherapy in oral squamous cell carcinoma.
Objective To investigate the effect of mitochondrial fission protein 1(FIS1) on apoptosis and cisplatin resistance in tongue squamous cell carcinoma(TSCC) cells. Methods The squamous cell carcinoma cell lines SCC9 and CAL27 were used to detect the mRNA and protein levels of FIS1 after cisplatin treatment, the knockdown and overexpression of FIS1 of SCC9 and CAL27 with or without cisplatin treatment were accomplished through small interfering RNA(siRNA) and plasmid, respectively. The mitochondrial division state in cells was detected by mitochondrial staining, and the apoptosis state of cells was detected by TUNEL, flow cytometry and Caspase 3/7. Results FIS1 protein expression in tongue squamous carcinoma cells treated with cisplatin was increased, but the mRNA level did not change.Silencing of FIS1 expression reduced mitochondrial division and apoptosis in squamous cell carcinoma cells treated with cisplatin, whereas overexpression of FIS1 exhibited the opposite effects. The percentage of dividing mitochondria,the number of apoptotic cells and the activity of Caspase 3/7 in SCC9 and CAL27 cells were significantly different before and after modulation of FIS1 expression(P < 0.05). Conclusion FIS1 is involved in the regulation of cisplatin chemotherapy sensitivity in tongue squamous cell carcinoma and can be used as a new target for improving the sensitivity of cisplatin chemotherapy in oral squamous cell carcinoma.
引文
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[13]Friedman JR,Lackner LL,West M,et al.ER tubules mark sites of mitochondrial division[J].Science,2011,334(6054):358-362.
[2]Mishra P,Chan DC.Mitochondrial dynamics and inheritance during cell division,development and disease[J].Nat Rev Mol Cell Biol,2014,15(10):634-646.
[3]Galluzzi L,Senovilla L,Vitale I,et al.Molecular mechanisms of cisplatin resistance[J].Oncogene,2012,31(15):1869-1883.
[4]Zhong LP,Zhang CP,Ren GX,et al.Randomized phase III trial of induction chemotherapy with docetaxel,cisplatin,and fluorouracil followed by surgery versus Up-Front surgery in locally advanced resectable oral squamous cell carcinoma[J].J Clin Oncol,2013,31(6):744-751.
[5]Wang JX,Jiao JQ,Li Q,et al.miR-499 regulates mitochondrial dynamics by targeting calcineurin and dynamin-related protein-1[J].Nat Med,2011,17(1):71-78.
[6]Tanaka A,Youle RJ.A chemical inhibitor of DRP1 uncouples mitochondrial fission and apoptosis[J].Mol Cell,2008,29(4):409-410.
[7]Srinivasan S,Guha M,Kashina A,et al.Mitochondrial dysfunction and mitochondrial dynamics-the cancer connection[J].Biochim Biophys Acta Bioenerg,2017,1858(8,SI):602-614.
[8]Trotta AP,Chipuk JE.Mitochondrial dynamics as regulators of cancer biology[J].Cell Mol Life Sci,2017,74(11):1999-2017.
[9]Zorzano A,Isabel Hernandez-Alvarez M,Sebastian D,et al.Mitofusin 2 as a driver that controls energy metabolism and insulin signaling[J].Antioxid Redox Signal,2015,22(12):1020-1031.
[10]Li J,Wang YL,Wang YP,et al.Pharmacological activation of AMPK prevents Drp1-mediated mitochondrial fission and alleviates endoplasmic reticulum stress-associated endothelial dysfunction[J].J Mol Cell Cardiol,2015,86:62-74.
[11]Kashatus JA,Nascimento A,Myers LJ,et al.Erk2 phosphorylation of Drp1 promotes mitochondrial fission and MAPK-driven tumor growth[J].Mol Cell.2015,57(3):537-551.
[12]Wan YY,Zhang JF,Yang ZJ,et al.Involvement of Drp1 in hypoxia-induced migration of human glioblastoma U251 cells[J].Oncol Rep,2014,32(2):619-626.
[13]Friedman JR,Lackner LL,West M,et al.ER tubules mark sites of mitochondrial division[J].Science,2011,334(6054):358-362.