10%核型甜菜夜蛾多角体病毒·甲维盐水悬浮剂的初步研制
|
摘要
以核型甜菜夜蛾多角体病毒(seNPV)和甲维盐复配,制备水悬浮剂农药。这种生物与化学农药复配的组合一定程度上弥补了生物农药价格昂贵、起效慢的缺点。通过饲料染药法得到seNPV的LC50为1.88×105 PIB/mL,甲维盐LC50为1.9185 mg/L。通过共毒因子配伍法确定seNPV·甲维盐复配组合具有增效作用,并初步确定了LC50浓度下体积比80∶1～350∶1为增效区间。以共毒系数法确定了两种药剂在各自LC50浓度下体积比为200∶1时效果最佳,增效倍数为0.9841。通过两种方法的联用,提高了实验效率。利用优化组合法对seNPV·甲维盐水悬浮剂助剂配方进行筛选,通过测定不同配方的叶片持留量、表面张力和悬浮率,并进行冷储、热储实验,确定农药最佳配方为:seNPV6.62%、甲维盐3.38%、十二烷基硫酸钠6%、对苯乙烯磺酸2%、有机硅4%、硅酸镁铝1%、黄原胶0.5%,水补足至100%
In this paper,the aqueous suspension pesticides were prepared by combining the Spodoptera exigua nuclera polyhedrosis virus( seNPV) and emamectin benzoate. This combination of biological and chemical pesticides to a certain extent makes up for the shortcomings of expensive and slow-acting biological pesticides. The LC50 of seNPV obtained by feed-dose method was 1.88×105 PIB/mL,and the LC50 of emamectin benzoate was 1.9185 mg/L.Co-toxicity factor was used to evaluate the formulation synergism,and the efficiency range was 80∶1～350∶1. Cotoxicity coefficient method was adopted to screen the optimum ratio of pesticides,and the two agents had the best effect when their volume ratio of LC50 was 200∶1. The synergistic multiple was 0.9841. Through the combination of two methods,the experimental efficiency was improved. The optimized composition method was used to screen the formulation of the SeNPV·mamectin benzoate suspension concentrate formulation. By determining the retention,surface tension and suspension rate of different formulations on the leaves,and performing cold storage and heat storage experiments,the optimum formulation of pesticides was determined to be :seNPV 6.62%,mamectin benzoate3.38%,sodium lauryl sulfate 6%,p-styrene sulfonic acid 2%,silicone 4%,magnesium silicate aluminum 1%,xanthan gum 0.5%,water to 100%.
In this paper,the aqueous suspension pesticides were prepared by combining the Spodoptera exigua nuclera polyhedrosis virus( seNPV) and emamectin benzoate. This combination of biological and chemical pesticides to a certain extent makes up for the shortcomings of expensive and slow-acting biological pesticides. The LC50 of seNPV obtained by feed-dose method was 1.88×105 PIB/mL,and the LC50 of emamectin benzoate was 1.9185 mg/L.Co-toxicity factor was used to evaluate the formulation synergism,and the efficiency range was 80∶1～350∶1. Cotoxicity coefficient method was adopted to screen the optimum ratio of pesticides,and the two agents had the best effect when their volume ratio of LC50 was 200∶1. The synergistic multiple was 0.9841. Through the combination of two methods,the experimental efficiency was improved. The optimized composition method was used to screen the formulation of the SeNPV·mamectin benzoate suspension concentrate formulation. By determining the retention,surface tension and suspension rate of different formulations on the leaves,and performing cold storage and heat storage experiments,the optimum formulation of pesticides was determined to be :seNPV 6.62%,mamectin benzoate3.38%,sodium lauryl sulfate 6%,p-styrene sulfonic acid 2%,silicone 4%,magnesium silicate aluminum 1%,xanthan gum 0.5%,water to 100%.
引文
[1]张小丽,陈萍,陈翠芳,等.转Bt基因抗虫棉对甜菜夜蛾实验种群增长的影响[J].植物保护学报,2007,34(4):391-395.
[2]曹春霞,杨妮娜,程贤亮,等.十字花科蔬菜主要病虫害生物防治技术[J].湖北农业科学,2016,55(22):5811-5814.
[3]曹立耘.甜菜夜蛾在棉区的危害特点与防治[J].农药市场信息,2014(17):48.
[4]崔劲松,梅爱中,许海蓉,等.甜菜夜蛾发生规律与控制技术[J].上海蔬菜,2015(6):53-54.
[5]王春兰,林双喜,丁志宽,等.不同低毒药剂防治萝卜甜菜夜蛾药效试验[J].蔬菜,2018(4):44-47.
[6]孟祥坤,朱超,于新,等.高毒力白僵菌的筛选及对花生甜菜夜蛾的致病力[J].中国生物防治学报,2018(2):259-265.
[7]杨建云,曹溪,纪春艳,等.粘质沙雷氏菌菌株PS-1对甜菜夜蛾种群增长的抑制作用[J].中国生物防治学报,2015(4):501-507.
[8]金仙花.论农药危害性及绿色植保技术[J].农业与技术,2017(8):26.
[9]邵天玉,刘兴龙,刘思竹,等.我国甜菜夜蛾核型多角体病毒研究新进展[J].黑龙江农业科学,2016(1):162-166.
[10]汤历,江敏华,徐树兰,等.10亿PIB/mL甜菜夜蛾核型多角体病毒悬浮剂的配方研制与应用[J].广东农业科学,2011,38(15):65-67.
[11]李国平,封洪强,黄博,等.应用瓶膜法和人工饲料混合法测定比较16种杀虫剂对绿盲蝽的毒力[J].昆虫学报,2017,60(6):650-658.
[12]陆小军,程东美,汤历,等.10种杀虫剂对斜纹夜蛾三龄幼虫的毒力测定[J].广东农业科学,2007(3):52-54.
[13]金剑雪,金道超,李文红,等.防治白背飞虱的农药复配增效配方筛选[J].植物保护,2017,43(1):199-204.
[14]顾中言,陈志谊,刘邮洲.分析增效系数和共毒系数的异同及探讨杀菌剂混用联合作用的评判[J].江苏农业学报,2016,32(6):1262-1267.
[15]庄占兴,胡尊纪,范金勇,等.噻虫胺与高效氯氟氰菊酯复配防治韭菜迟眼蕈蚊幼虫配方筛选与田间应用效果[J].农药,2017(3):228-230.
[16]金党琴,龚爱琴.印楝素和银杏酚酸对甜菜夜蛾幼虫的协同增效作用[J].西北农业学报,2017,26(9):1402-1406.
[17]薛背背,路福绥,骆燕,等.溶剂替换法制备氟铃脲水悬浮剂及其分散稳定性[J].应用化学,2011(1):72-76.
[18]王龙江,廖永林,黄少华,等.不同助剂对甲维盐微乳剂在水稻叶片上附着性能的影响[J].环境昆虫学报,2017(2):419-422.[1 9]逄森,袁会珠,李永平,等.表面活性剂Silwet408提高药液在蔬菜叶片上润湿性能的研究[J].农药科学与管理,2005(7):22-25.
[2]曹春霞,杨妮娜,程贤亮,等.十字花科蔬菜主要病虫害生物防治技术[J].湖北农业科学,2016,55(22):5811-5814.
[3]曹立耘.甜菜夜蛾在棉区的危害特点与防治[J].农药市场信息,2014(17):48.
[4]崔劲松,梅爱中,许海蓉,等.甜菜夜蛾发生规律与控制技术[J].上海蔬菜,2015(6):53-54.
[5]王春兰,林双喜,丁志宽,等.不同低毒药剂防治萝卜甜菜夜蛾药效试验[J].蔬菜,2018(4):44-47.
[6]孟祥坤,朱超,于新,等.高毒力白僵菌的筛选及对花生甜菜夜蛾的致病力[J].中国生物防治学报,2018(2):259-265.
[7]杨建云,曹溪,纪春艳,等.粘质沙雷氏菌菌株PS-1对甜菜夜蛾种群增长的抑制作用[J].中国生物防治学报,2015(4):501-507.
[8]金仙花.论农药危害性及绿色植保技术[J].农业与技术,2017(8):26.
[9]邵天玉,刘兴龙,刘思竹,等.我国甜菜夜蛾核型多角体病毒研究新进展[J].黑龙江农业科学,2016(1):162-166.
[10]汤历,江敏华,徐树兰,等.10亿PIB/mL甜菜夜蛾核型多角体病毒悬浮剂的配方研制与应用[J].广东农业科学,2011,38(15):65-67.
[11]李国平,封洪强,黄博,等.应用瓶膜法和人工饲料混合法测定比较16种杀虫剂对绿盲蝽的毒力[J].昆虫学报,2017,60(6):650-658.
[12]陆小军,程东美,汤历,等.10种杀虫剂对斜纹夜蛾三龄幼虫的毒力测定[J].广东农业科学,2007(3):52-54.
[13]金剑雪,金道超,李文红,等.防治白背飞虱的农药复配增效配方筛选[J].植物保护,2017,43(1):199-204.
[14]顾中言,陈志谊,刘邮洲.分析增效系数和共毒系数的异同及探讨杀菌剂混用联合作用的评判[J].江苏农业学报,2016,32(6):1262-1267.
[15]庄占兴,胡尊纪,范金勇,等.噻虫胺与高效氯氟氰菊酯复配防治韭菜迟眼蕈蚊幼虫配方筛选与田间应用效果[J].农药,2017(3):228-230.
[16]金党琴,龚爱琴.印楝素和银杏酚酸对甜菜夜蛾幼虫的协同增效作用[J].西北农业学报,2017,26(9):1402-1406.
[17]薛背背,路福绥,骆燕,等.溶剂替换法制备氟铃脲水悬浮剂及其分散稳定性[J].应用化学,2011(1):72-76.
[18]王龙江,廖永林,黄少华,等.不同助剂对甲维盐微乳剂在水稻叶片上附着性能的影响[J].环境昆虫学报,2017(2):419-422.[1 9]逄森,袁会珠,李永平,等.表面活性剂Silwet408提高药液在蔬菜叶片上润湿性能的研究[J].农药科学与管理,2005(7):22-25.