瘤毛獐牙菜体细胞胚胎发生过程中生理生化特性研究
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摘要
目的从生理生化层面探讨瘤毛獐牙菜体细胞胚胎发生过程中生理生化指标与愈伤组织生长发育的关系,并确定愈伤组织最佳继代时间。方法自转接分化培养基起,每隔7 d测定愈伤组织内可溶性糖、可溶性蛋白含量;超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)活性。结果瘤毛獐牙菜体细胞胚胎发生过程中,愈伤组织内可溶性糖、可溶性蛋白含量在35 d左右达到最高值; SOD、POD活性整体呈上升趋势,PPO在14-35 d间活性相对较低。结论根据生理生化指标在体细胞胚胎发生过程中呈现出的变化趋势可推断愈伤组织的生长发育情况;愈伤组织最佳继代时间为28-35 d。
Objective: To study the relationship between physiological and biochemical indexes and callus growth and development during somatic embryogenesis of swertia pseudochinensis and determine the best subculture time of callus. Method: The contents of soluble sugar and protein,the activities of superoxide dismutase(SOD),peroxidase(POD) and polyphenol oxidase(PPO) in the callus were determined every 7 days from the transfer differentiation medium. Result: During somatic embryogenesis,the content of soluble sugar and soluble protein in callus reached the highest value in about 35 days; the activity of SOD and POD increased as a whole,and the activity of PPO was relatively low in14-35 d. Conclusion: According to the trend of physiological and biochemical indicators in the process of somatic embryogenesis,the growth and development of callus can be inferred; the best subculture time of callus is 28-35 d.
Objective: To study the relationship between physiological and biochemical indexes and callus growth and development during somatic embryogenesis of swertia pseudochinensis and determine the best subculture time of callus. Method: The contents of soluble sugar and protein,the activities of superoxide dismutase(SOD),peroxidase(POD) and polyphenol oxidase(PPO) in the callus were determined every 7 days from the transfer differentiation medium. Result: During somatic embryogenesis,the content of soluble sugar and soluble protein in callus reached the highest value in about 35 days; the activity of SOD and POD increased as a whole,and the activity of PPO was relatively low in14-35 d. Conclusion: According to the trend of physiological and biochemical indicators in the process of somatic embryogenesis,the growth and development of callus can be inferred; the best subculture time of callus is 28-35 d.
引文
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[2]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80-84.
[3]张福生,郭顺星. SPSS正交设计在福建金线莲组织培养中的应用[J].中国中药杂志,2009,34(20):2581-2585.
[4]谢伟杰,张永萍,徐剑,等.铁皮石斛现代研究进展[J].现代中医药,2016,36(4):87-91.
[5]周艳,陈训.马缨杜鹃组织培养过程中外植体褐变与多酚氧化酶及酚类物质的关系[J].种子,2009,28(7):61-63.
[6]詹园凤,吴震,金潇潇,等.大蒜体细胞胚胎发生过程中抗氧化酶活性变化及某些生理特征[J].西北植物学报,2006,26(9):1799-1802.
[7]许传营,陈星旭,辛伟杰,等.花烛体细胞胚胎发生过程中相关生理生化特征研究[J].广西植物,2010,30(1):117-121.
[8]张建瑛,杨玲,沈海龙.花楸体细胞胚发生过程中抗氧化酶活性的变化[J].植物生理学报,2007,43(2):264-268.
[9]Jariteh M,Ebrahimzadeh H,Niknam V,et al. Developmental changes of protein,proline and some antioxidant enzymes activities in somatic and zygotic embryos of Persian walnut(Juglans regia L.)[J]. Plant Cell Tissue&Organ Culture,2015,122(1):101-115.
[10]温银元,王玉国,扆铁梅.银杏胚愈伤组织的继代培养及其生理特性研究[J].山西农业大学学报(自然科学版),2008,28(3):263-266.
[11]何丽君,邬海波.半日花体细胞胚胎发生过程中超氧物歧化酶(SOD)活性的变化[J].内蒙古科技与经济,2000(S1):390-392.
[12]罗晓丽,王志安,肖娟丽,等.不同继代时间调控棉花愈伤组织褐化死亡和植株再生效率的研究[J].生物技术进展,2013,3(3):201-205+235.